List of Articles three spot gourami

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  1 - The Effect of Cucurbita pepo extract and 17β-estradiol(E2) on HPG axis in Three-spot gourami (Trichogaster tricopterus)
  T. Naji H. Hosseinzadeh Sahafi S. Omidi
  The   effect  of   Cucurbita  pepo  extract and  17β-estradiol(E2) on  HPG  axis  was  investigated  in  male  Three  spot  gourami. The purpose of this research was evaluated effe More

  The   effect  of   Cucurbita  pepo  extract and  17β-estradiol(E2) on  HPG  axis  was  investigated  in  male  Three  spot  gourami. The purpose of this research was evaluated effect of Cucurbita  pepo on sex steroids level and measurement of blood calcium and also changes of liver tissue. Therefore, 100specimen of immature male fish with mean weight (2.5±0.5g) were evaluated in 10 treatments. The fishes in different groups were treated with Cucurbita pepo extract and the E2 separately. (10, 20, 30,50mg/Kg body weight).There was two groups as controls. Control I received 20µL methanol and control II was intact. After 20 days experimental, fishes were dissected. To measure the steroid hormones and calcium ion, tissue fluids were separated and the level of estradiol, testosterone and calcium ion analyzed. Results showed that level of sex steroid and Ca+2,for Cucurbita  pepo extract in treatments I and II were 1.1,0.83,19.73 , for treatment III were 1.34,1.4,20.65 , for treatment IV were 1.8,1.68,22.86 and level of sex steroid and Ca+2, for (E2) in treatment V were 0.88,2.55,24.43and VI treatment was 0.91,3.54,27.15 and for treatment VII were 0.97,4.41,30.80, for treatment VIII were 1.08,6.45,35.32 in comparison with control group(0.78,0.52,16.46)that were significant (P<0.05). These results showed that treatments IV and VIII were more affected by level steroids and   Ca +2 ions in male Three spot gourami. The overall conclusion is that extract of Cucurbita  pepo could possibly induce sexual maturity in male Three spot gourami. Manuscript profile
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  2 - Effects of Silymarin and 17β estradiol on gonadosomatic index and histological changes of oocytes in female Three spot Gourami. (Trichogaster trichopterus)
  T. Naji H. Hosseinzadeh sahafi S. Rezaee
  Effects of silymarin and 17β estradiol (E2) on gonadosomatic index and histological changes of oocytes were investigated in female Three spot Gourami. 100 immature female fish with mean weight of (2.5±0.5g) were evaluated in 8 treatments with 2 control group More

  Effects of silymarin and 17β estradiol (E2) on gonadosomatic index and histological changes of oocytes were investigated in female Three spot Gourami. 100 immature female fish with mean weight of (2.5±0.5g) were evaluated in 8 treatments with 2 control groups. The fishes in different groups were treated with silymarin and E2 separately (10,20,30,50 mg/kg). Control one received 20µL methanol and control two was intact. After 20 days experimental fishes ovaries removed and weighted. Histopathological studies of ovary and gonadosomatic indires of fishes of the 8 tretments were compared with those of control groups, fishes. Statistical analysis showed that the results of comparison of amount of GSI for silymarin in first and second treatments was 1.2% and for third and fourth treatments was 1/8% Amount of GSI for (E2) in fifth treatment was 0.7% and sixth treatment was 1.2% and for seventh and eighth treatments it was 1.75% in comparison with control group which was 0.5% these defferences were significant (P<0.05). This result was approved by histopathological finding which showed that fourth and eighth treatment were more effective because begining moving Germinal vesicle on growth and maturation of oocytes in female three Spot Gourami. Manuscript profile
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  3 - Comparison between phytoestrogen Trigonella foenum- gracum L seed and 17 β estradiol, on ovarian and hepatic tissues of female immature three spot gourami
  طاهره Naji, P. Shahruzifar, H. Hosseinzade
  Studies shows that Fenugreek is due to estrogenic compounds phytoestrogenic properties.In this study, it is used Fenugreek seed extract as a phytoestrogen and 17β estradiol (E2) on ovarian and liver tissues of female spot gourami. For this purpose, the fshes were More

  Studies shows that Fenugreek is due to estrogenic compounds phytoestrogenic properties.In this study, it is used Fenugreek seed extract as a phytoestrogen and 17β estradiol (E2) on ovarian and liver tissues of female spot gourami. For this purpose, the fshes were released to chlorine less aquarium water, their weighted average was between 1.5 ± 0.5 g. They were kept for one week in same condition for adaptation with new condition. These experiments were performed in ten groups (two control groups and eight treated groups); each group contained ten immature of female fshes. For 20 days, Fenugreek extract and E2 (following doses 10,20,30,50 mg/kg) were injected separately under dorsal fn, injection method was IM (Inter muscular). After 24 hours, ovary and liver tissues were taken, after passage of texture and staining, the tissues were examined under microscope and were compared with control treatment. Then using the software programs like SPSS and statistical tests like DUNCON and ANOVA, Ovarian and liver tissue samples were compared with different treatments as well as Gonadosomatic and hepatosomatic indices were measured. Signifcant differences in treatment of GSI and HSI treated with Trigonella foenum-graceum L and E2 was observed in control groups. Results showed that increasing the dose of fenugreek extract and E2 GSI, HSI and diameter of oocytes increased but this increase was more signifcant in E2.   Manuscript profile