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        1 - Association of progesterone concentration and lactation number on percentage of endometrial polymorphonuclear cells in dairy cows
        Dini, P., FarhoodiMoghadam, M.*, Fazeli, M.H., Akbari, G. .
        The objectives of this study were to assess the association between progesterone concentration and lactation number with the percentage of endometrial polymorphonuclear cells (PMN) obtained by low volume lavage (LVL) for the diagnosis of subclinical endometritis in dair More
        The objectives of this study were to assess the association between progesterone concentration and lactation number with the percentage of endometrial polymorphonuclear cells (PMN) obtained by low volume lavage (LVL) for the diagnosis of subclinical endometritis in dairy cows. Dairy cows at 30days in milk (DIM), were clinically examined and reproductive tract were evaluated for any abnormality. Cows (n=150) with sound reproductive tract were included in this study.  Endometrial cytology (LVL) and blood (tail vessels) samples were obtained at 30 DIM and at 40 DIM, and used for measuring percentage of PMN and Progesterone concentration, respectively. Two-blinded operators determined the percentage of PMN obtained by cytology. Data were analyzed with PROC FREQ, PROC CORR and PROC GLIMIX, in SAS 9.1. There was a significant negative correlation between serum progesterone concentration and percentage of PMNs (r = -0.58, P<0.001). Lactation numbers >2 and peripheral progesterone concentrations >1 ng/mL were significantly associated with lower PMN percentages at 40 DIM (P = 0.0041, 0.0187, respectively). According to the current study, cows with higher serum progesterone level, had lower chance of displaying subclinical endometritis assessing with the evaluation of PMNs% in the uterus at 40 DIM. This finding can be explained by the fact that the cows with higher serum progesterone level start estrous cycle earlier, which is prerequisite step for cleaning the postpartum uterus. Manuscript profile
      • Open Access Article

        2 - Assessment of endometrial cytology to diagnose subclinical endometritis and its effects on fertility in repeat breeder dairy cows
        بهرام Salasel. A. Mokhtari تقی Taktaz. M. Khosravi.
        The objective of this study was to describe endometrial cytology to determine the prevalence of subclinicalendometritis (SCE) and iťs impacts on fertility in Repeat breeder dairy cows.Dairy cows parity 1 to 5 which were inseminated 3 or more times (the average number of More
        The objective of this study was to describe endometrial cytology to determine the prevalence of subclinicalendometritis (SCE) and iťs impacts on fertility in Repeat breeder dairy cows.Dairy cows parity 1 to 5 which were inseminated 3 or more times (the average number of AI in selected cows was3.8) were examined in 190±40 days in milk. Clinically normal cows (n=77) were selected based on the absenceof abnormal discharges on external inspection and also absence of abnormal fndings on transrectal palpation andultrasonographic examination.Endometrial samples were collected from the uterus using the lavage technique in the luteal phase of estrus cycle.Collected solution centrifuged and a drop of sediment was streaked on to a clean microscopic slide and stained withGiemsa. The percentage of polymorphonuclear cells(neutrophils) was counted for each specimen.Analyzed data showed that in the selected cows, the mean amount of neutrophils was 3/1 %(0-9) and the prevalenceof cytologically diagnosed SCE (based on more than 2% neutrophils) was 52/7 %(n=38).Cytologically diagnosed SCE was signifcantly associated with impaired reproductive performance.In this study, 25% of cows (n=18) became pregnant in the next service. Conception rate (in the next AI) was 5% forcows (n=38) with SCE (more than 2% neutrophils), and 47% for cows (n=34) without SCE(less than 3% neutrophils)(p=0.009).In conclusion, subclinical endometritis in repeat breeder cows which diagnosed by endometrial cytology wasassociated with reduced reproductive performance such as relative conception rate and increased risk of involuntaryor premature culling. Manuscript profile