List of Articles sea gene

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  1 - Molecular Detection of Methicillin Resistant Enterotoxin a Producing Staphylococcus aureus in Profiteroles in Karaj in 2016
  M. Makvandi N. Harzandi
  Introduction: Staphylococcal enterotoxins are members of a family of more than 20 different staphylococcal and streptococcal exotoxins that are functionally related and share corresponding gene sequence homology. These bacterial proteins are known to be mitogen and are More

  Introduction: Staphylococcal enterotoxins are members of a family of more than 20 different staphylococcal and streptococcal exotoxins that are functionally related and share corresponding gene sequence homology. These bacterial proteins are known to be mitogen and are responsible for significant human diseases including food poisoning. The aim of this study was to detect the enterotoxin A producing Staphylococcus aureus in profiteroles.Materials and Methods: Sampling was carried out from 50 confectionaries in Karaj from mid-August 2016 to February 2017. Serial dilutions from each sample were prepared and cultured on Baird parker agar. After 48 hours incubation at 37ºC, black colonies were selected. Following transfer to blood agar, susceptibility of isolates to oxacillin was determined using disc diffusion method and presence of nuc, mecA and sea genes was checked using PCR.Results: In total, based on culture results 8 out of 50 samples (16%) had Staphylococcus aureus contamination. PCR method results showed the presence of nuc gene in 14 (28%) of the samples, mecA gene and sea gene in 5 (10%), 3 (6%) of the samples respectively.Conclusion: Remarkable frequency of enterotoxin A producing MRSA isolates in different foods and specially profiteroles as an enrichment medium for growth and toxin production of bacteria, is a warning for public health. Manuscript profile
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  2 - Molecular evaluation of sea enterotoxin gene expression in Staphylococcus aures treated with Ginkgo biloba and Zingiber officinale plant extracts
  Shideh Masoumi Hadi Habibollahi Mohammad Reza Safari Motlagh
  10.30495/jdb.2023.1960528.1309
  In this study, the antimicrobial effect of extracts of Ginkgo biloba and Zingiber officinale plants against Staphylococcus aureus and their effect on the expression of sea enterotoxin gene were investigated. Two standard and pathogenic strains of S. aureus with enteroto More

  In this study, the antimicrobial effect of extracts of Ginkgo biloba and Zingiber officinale plants against Staphylococcus aureus and their effect on the expression of sea enterotoxin gene were investigated. Two standard and pathogenic strains of S. aureus with enterotoxin sea gene and G. biloba and Z. officinale extracts were used as ready-made tablets with a specified concentration. Antimicrobial test of the extracts was performed by disk impregnation and then the minimum inhibitory and bacteriocidal concentrations of these extracts were evaluated by MIC and MBC tests and SubMIC concentration of these extracts was used to evaluate the effect of extract treatment on the expression of sea enterotoxin gene. Then RNA extraction and cDNA synthesis and finally Real time PCR were performed and the results were interpreted. The results of disk diffusion and MIC showed that Z. officinale extract has antimicrobial effect. Interpretation of Real time PCR results also showed that Z. officinale extract reduced and completely inhibited the expression of sea enterotoxin gene in standard strain ATCC 25923. This extract also significantly reduced the expression of the target gene in the pathogenic strain, and its expression was reduced to 4% compared to the untreated sample. On the contrary, G. biloba extract significantly increased sea gene expression. This extract increased the expression of the target gene in the standard strain and the pathogen by approximately 55 and 9 fold, respectively. According to the results of this study, ginger can be a good candidate to fight S. aureus infections. Manuscript profile