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      • Open Access Article

        1 - Effect of Echinacea pupurea essential oil on growth and Aspergillus flavous mycotoxin production
        Arezoo Malakouti Anousheh Sharifan Alireza Basiri
        Foodborne diseases contaminated with mycotoxins are a concern for human health. The use of bioactive compounds to improve food safety and preservation has been considered to reduce the economic losses and risks of these factors. The aim of this study was to use purple c More
        Foodborne diseases contaminated with mycotoxins are a concern for human health. The use of bioactive compounds to improve food safety and preservation has been considered to reduce the economic losses and risks of these factors. The aim of this study was to use purple coneflower (Echinacea purpurea) essential oil as a bio preservative to control the growth of Aspergillus flavus and to prevent the production of aflatoxin B1. For this purpose, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values were calculated using a disk diffusion method and then the effect of different concentrations of the essential oil on aflatoxin B1 production was evaluated. The amount of aflatoxin B1 production in the YES (yeast extract sucrose) culture media was measured by HPLC. The results of this study showed that MIC and MFC were 10000 ppm and 15000 ppm, respectively. The results of the growth inhibition zone showed that increasing concentration of the essential oil significantly (p<0.05) increased the diameter of the inhibition zone. It was also founded that aflatoxin B1 production was significantly (p<0.05) dependent on purple coneflower concentration so that by increasing of the essential oil concentration the aflatoxin production significantly (p<0.05) decreased. Accordingly, the aflatoxin B1 production by A. flavus was prevented using concentration of 15000 ppm of purple coneflower essential oil. Overall, it can be concluded that purple coneflower essential oil can control the production of aflatoxin by A. flavus to an acceptable level Manuscript profile
      • Open Access Article

        2 - Effect of organic and chemical fertilizers along with silicon application on morpho-physiological characteristics and yield of purple coneflower (Echinacea purpurea L.)
        Hasan Yahyapoor Yousof Niknezhad
        This research was conducted with aim of investigating the effects of organic and chemical fertilizers along with silicon on morpho-physiological characteristics and yield of purple coneflower (Echinacea purpurea L.), in Mazandaran,, Amol during 2022. The experiment was More
        This research was conducted with aim of investigating the effects of organic and chemical fertilizers along with silicon on morpho-physiological characteristics and yield of purple coneflower (Echinacea purpurea L.), in Mazandaran,, Amol during 2022. The experiment was cconducted as a split-plot in a randomized complete block design with three replications. The experimental treatments include the application of organic and chemical fertilizers at four levels (control, manure, vermicompost and chemical fertilizer) as the main plot and silicon at three levels (control, potassium silicate and nano-Si) were considered as sub plot. The results indicated that the most growth characteristics like plant height, number of flowers per plant and leaf area were obtained by chemical fertilizer. The highest dry weight of plant organ was achieved with use of chemical fertilizers. The highest dry weight of flowers (429.3 g.m-2) was obtained by chemical fertilizer, which was not significantly different from the use of vermicompost (417.3 g.m-2). The application of both sources of silicon led to improvement in the number of flowers per plant, leaf area, and concentration of chlorophylls compared with control, although the application of nano-Si had higher improvement effects compared with potassium silicate. The nano-Si increased the dry weight of flowers by 9.6 and 7.4% compared with non-application of silicon and potassium silicate, respectively. Therefore, according to the findings of the present study, application of vermicompost organic fertilizer is introduced as a suitable alternative to chemical fertilizers, as well as nano-Si application to improve the morpho-physiological characteristics and yield of purple coneflower. Manuscript profile