This study aims to examine serumic indices of renal function in horses suffering from piroplasmosis and to compare them with healthy animals. Based on clinical signs and laboratory findings, 17 horses (14 males and 3 females) were diagnosed with piroplasmosis in horse r More
This study aims to examine serumic indices of renal function in horses suffering from piroplasmosis and to compare them with healthy animals. Based on clinical signs and laboratory findings, 17 horses (14 males and 3 females) were diagnosed with piroplasmosis in horse ranches of the suburb of Tabriz city. After taking the history of these animals, blood sample was taken from the jugular vein and serum was separated. Also, 16 healthy horses (13 males and 3 females) were sampled as a healthy group with the same age, feeding and managerial conditions. Renal function was evaluated based on serumic level of blood urea nitrogen (BUN), creatinine (Cr), total protein (Pr), albumin (Alb), sodium (Na) and potassium (K). Mean serumic level of total protein and albumin had an insignificant increase in horses suffering from piroplasmosis, but increment of mean serumic level of creatinine and BUN was significant in the diseased group (p=0.000 and p=0.001, respectively). Serum variations of sodium and potassium were not significant between the two groups. The difference between mean serum parameters in diseased group was significant between the two sexes only in respect to total protein and albumin (p=0.001 and p=0.012, respectively). In healthy group, age did not have any effect on serum variations of measured parameters, but in the diseased group, mean serum total protein increased with increase in age (p=0.033). The results of this study indicated that renal function is affected in horses suffering from piroplasmosis and improvement of renal function using fluid therapy should be considered in the treatment of this disease.
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In the present study the rate of Theileria and Babesia spp. infection of sheep housing in Khoramabad, Iran was determined by using PCR technique and traditional giemsa staining method. DNA was extracted from blood samples that collected from 100 sheep from five regions More
In the present study the rate of Theileria and Babesia spp. infection of sheep housing in Khoramabad, Iran was determined by using PCR technique and traditional giemsa staining method. DNA was extracted from blood samples that collected from 100 sheep from five regions of Khoramabad and amplified by using specific primers derived from 18S rRNA gene of the mentioned piroplasms by PCR technique and subsequently analyzed on agarose gel using ethidium bromide under UV condition. Additionally, the prepared blood smears of each sheep were analyzed by Giemsa staining method. The results showed that structures assembling the mentioned piroplasms were detectable in 15 blood smears. Twelve of them were determined as Theileria spp. and the remaining three as Babesia spp. The PCR results showed that 28 samples were positive. The PCR product specific for Theileria spp. amplified in 22 samples (22%). Babesia spp. were detected in 6 blood samples (6%). The apparent agreement between two tests was 81%. When PCR technique is considered as golden standard method, the sensitivity and specificity of the other method were measured as 42% and 95.8%, respectively.
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This study was performed during the one year period, from March 21st 2011 to 2012, for detection v infection rate of Theileriosis in Borujerd township, and 860 cows were tested (220 samples in each season).
First, the data related to every cow was recorded in a questio More
This study was performed during the one year period, from March 21st 2011 to 2012, for detection v infection rate of Theileriosis in Borujerd township, and 860 cows were tested (220 samples in each season).
First, the data related to every cow was recorded in a questionnaire. Second, all cows were clinically examined and the positive cases which had Lymphadenitis, icterus in the vaginal mucosa and eye, fever, constipation, hyperpnea, diarrhea, coughing, watery eyes and mouth were distinguished and depleted from ear and forthwith were fixed by ethyl alcohol. Last, the blood smears samples were stained by Giemsa method and tested from view point of presence of piroplasmosis figures.
To sum up, 536 of the 860 samples (62.32%) were positive and there were significant differences between male and female (P≤0.05). The most amount of infection was in spring (52.23%) and the least was in winter (0.93%) (P≤0.05).The most of infection rate was in over three years old cows (71.26%) and the least was in under one years old cows (3.54%) (P≤0.05).
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Background and purpose: Theileriosis is one of prevalent diseases among small ruminant population in Kurdistan province which is encountered serious problems both clinical and microscopic. The disease imposes heavy damages to the livestock industry. The aim of the study More
Background and purpose: Theileriosis is one of prevalent diseases among small ruminant population in Kurdistan province which is encountered serious problems both clinical and microscopic. The disease imposes heavy damages to the livestock industry. The aim of the study is to compare the clinical and microscopic features in sheep and goats infested with ticks, evaluation of microscopic method to identify Theileriosis and finally identification of technical impairments and finding confident approaches for diminished diagnostic errors in both methods. Material and methods: in this study, after recording the clinical findings of 250 tick infested small ruminants (230 sheep and 20 goats), blood and lymphatic smears were taken. Results: the results have shown that Piroplasms and Koch blue bodies were presented in 229 blood samples and in 238 lymphatic smears respectively. Statistical analyses declared that there was a meaningful correlation between blood and lymphatic smears and also between clinical findings such as: heart rate, conjunctiva discoloration and temperature variation with both blood and lymphatic smears. Conclusion: coordination of clinical and laboratory findings have confirmed that the main problem in clinical diagnosis of theileriosis in Kurdistan province was the involvement of animals with none-acute form of the disease (for i.e. T. ovis) in most population of small ruminants. Thus investigation of parasite both in blood smears and in lymphatic smears could lead to diminish laboratory errors.
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