List of Articles p24

• Open Access Article
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  1 - Effects of Targeting Subsidies on Agricultural Sector in Iran
  ahmad tashkini
  This study tries to examine the impact of liberalization on the price of energy carriers including gasoline, kerosene, gasoline, fuel oil, LPG, electricity and natural gas on agricultural sector in Iran by using Social Accounting Matrix. Despite of 13-20 percent of agri More

  This study tries to examine the impact of liberalization on the price of energy carriers including gasoline, kerosene, gasoline, fuel oil, LPG, electricity and natural gas on agricultural sector in Iran by using Social Accounting Matrix. Despite of 13-20 percent of agriculture contribution to GDP and employment, only 3.8 percent of total energy consumption is allocated to it during 2001-2009. The results of Social Accounting Matrix represent 57 and 41 percent growth in producer price index of agricultural products according to two scenarios (the average growth percentage of 2100 and 1570 in energy prices). Gasoline, by 40 percent of total direct and indirect effects, has the highest share in the increase of price index of agricultural production. To compensate the energy price liberalization on the agricultural sector, prolonging the rise of energy prices in the agricultural sector, preferential prices for electricity use in agriculture and energy efficiency in agriculture can be proposed. Manuscript profile
• Open Access Article
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  2 - Designing of HIV P24 Antigen assay kit using human monoclonal Antibody
  Masoomeh Hashemi Seyed-Mehdi Butorabi Reza Haji-Hoeini Ali Mir-Jalili
  Background and Objective: Several days after infection with HIV and before serum changes in the stage that there is RNA HIV in the blood, the antigen P24 of the HIV is measurable in the blood. The studies done show that in the primary stages of the infection, antigen p2 More

  Background and Objective: Several days after infection with HIV and before serum changes in the stage that there is RNA HIV in the blood, the antigen P24 of the HIV is measurable in the blood. The studies done show that in the primary stages of the infection, antigen p24 appears sooner than antibody; therefore, the evaluation of antigen p24 can be an appropriate index for diagnosing the infection in the first stages of the disease. Materials and Methods: 300 negative samples from blood donor were tested with the third-generation kit for determining the antibody against HIV. 30 positive samples were collected from the AIDS Research Center and the patients confirmed with Immunoblot and NAT methods. All the samples were investigated with the designed kit for antigen p24 in Elisa method. From among the samples, the samples of serums the antibody test of which were positive, pretreatment with different solutions including 1.5 molars of Buffer Glycin with PH=2, hydrochloric acid 0.5 N, Trition X-100 with a concentration of 0.1% , alkaline buffer No.1 and 2 for removing the probable interventional effect of antibody against antigen p24. Result: From among 30 positive samples, 21samples of antigen tests were positive in the kit designed by human monoclonal and 18 cases in the kit designed by mouse monoclonal antibody before pretreatment samples with Glycin (the diagnostic sensitivity is 70% and 60% respectively). After pretreatment, 28 samples in the kit designed by human monoclonal antibody and 27 samples in the kit designed by mouse monoclonal were positive.( the diagnostic sensitivity were 93% and 90% respectively). In the kit designed for detecting antigen p24, the cut off was determined on the basis of optical density of the negative samples 0.15(equal to 2 pg/ml of antigen P24). The difference between the average of optical density of the positive and negative samples in antigen test was significant statistically(pConclusion: This test has high sensitivity and specificity in diagnosing HIV and is more simple, fast, accurate, and economical in comparison with other diagnostic methods. This test may be used for screening newborns from the mothers with positive HIV and the taking decision about the cases for whom the fourth-generation of Elisa Tests are positive but Western Blot Test has not confirmed it. Manuscript profile