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        1 - Investigating in vitro culture of Abu Jahl watermelon plant Citrullus colocynthis (L.) Schrad
        سورمه Qara Matrosyan مه‌لقا Ghorbanli
        In this research, the determination of the best cultivation environment for stem organogenesis and the selection of the most suitable cultivar from Abu Jahl watermelon seeds have been studied. In order to obtain sterile roasted seeds, the inhibition of the seed shell wa More
        In this research, the determination of the best cultivation environment for stem organogenesis and the selection of the most suitable cultivar from Abu Jahl watermelon seeds have been studied. In order to obtain sterile roasted seeds, the inhibition of the seed shell was removed in concentrated sulfuric acid for 20 minutes. After washing with sterile distilled water, the seeds were transferred to a sterile culture medium. 20 days later, small pieces (cotyledon, terminal bud, cotyledon axis and root) were prepared from the roasted seeds and transferred to MS medium with different hormone treatments. All isolated cultures produced callus, and only the terminal bud treated with IAA and quintine equal to 1 mg in relitertolide produced callus, then it was differentiated into bud and branch. To determine the most suitable cultivation environment; Two cultivation environments were considered. MS basic culture medium with IAA and quintine treatment equal to 1 mg and MS basic culture medium, vitamin 2 equal to MS basic medium and benzyl aminopurine treatment 2 mg/liter along with naphthalene acetic acid 0.1 mg/liter. The terminal bud was separated from the sterile culture medium and placed in these two culture mediums. The design was based on a completely random block. Data collection was done in four stages. The ability to produce callus in the culture medium treated with benzyl aminopurine along with naphthalene acetic acid was higher than in the culture medium of IAA and quintine. In the cultivation environment with IAA and quintine treatment with equal amounts, branching and increase in the length of the branches were significant. In the culture medium, treatment with benzyl aminopurine along with naphthalene acetic acid caused the differentiation of callus into branches, but branching and increasing the length of branches were not significant. Manuscript profile
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        2 - ریز ازدیادی (کشت درون شیشه) کلیماتیس (Clematis orientalis L)
        علی ایزدی صادق آبادی احمد خلیقی عبدالله قاسمی پیربلوطی مرضیه تقی پوردهکردی
        مقدمه و هدف: کلیماتیس از گیاهان زینتی و دارویی است که تکثیر و ازدیاد آن از اهمیت زیادی برخوردار است. در این پژوهش اثر تنظیم‌کننده ‌های رشد بر ریشه زایی و رشد گیاه در شرایط کشت درون شیشه‌ای بررسی گردید. روش تحقیق: به منظور بررسی ریز ازدیادی کلیماتیس تأثیر هورمون‌های &nbs More
        مقدمه و هدف: کلیماتیس از گیاهان زینتی و دارویی است که تکثیر و ازدیاد آن از اهمیت زیادی برخوردار است. در این پژوهش اثر تنظیم‌کننده ‌های رشد بر ریشه زایی و رشد گیاه در شرایط کشت درون شیشه‌ای بررسی گردید. روش تحقیق: به منظور بررسی ریز ازدیادی کلیماتیس تأثیر هورمون‌های  NAA(نفتالین استیک اسید) در غلظت‌های ( 0، 4/0، 5/0 و 6/0 میلی‌گرم در لیتر) وIBA  (ایندول بوتیریک اسید) در غلظت‌های ( 0، 4/0، 5/0 و 6/0 میلی‌گرم در لیتر) با استفاده از کشت ریز نمونه های جانبی و انتهایی در شرایط درون شیشه‌ای حاوی محیط کشت پایه موراشیگی و اسکوگ (MS) بررسی گردید. نتایج و بحث: نتایج نشان داد که غلظت‌های مختلف اکسین بر رشد گیاهچه‌های درون شیشه معنی‌دار بود؛ به طوری که بیشترین درصد ریشه زایی و تعداد شاخه در گیاه شاهد مشاهده شده است. نتایج به دست آمده نشان داد که با اضافه نمودن هورمون به محیط کشت، ریشه زایی کاهش یافت. بیشترین کاهش با اضافه نمودن 6/0 میلی گرم در لیتر هورمون NAA به محیط کشت MS مشاهده گردید. هم­چنین استفاده از ریز نمونه های جانبی بر تعداد شاخه گیاه تأثیر معنی داری داشت و موجب افزایش تعداد شاخه گیاه گردید. توصیه کاربردی/ صنعتی: نتایج این تحقیق تاثیر مثبت ریز نمونه های جانبی و محیط کشت بدون هورمون را در افزایش ریزازدیادی کلیماتیس نشان داد. Manuscript profile
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        3 - Change in the content of bioactive pharmaceutical-industrial compounds under the influence of hormonal treatments In Euphorbia trigona Mill.
        Hakimeh Rezayi Aryan Sateei Tahereh A. Aghajanzadeh Mehdi Ebadi
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        4 - اثر تنظیم‌کننده‌های رشد، بستر کشت و نور در تولید سوخک سوسن رقم ꞋفوجیانꞋ با استفاده از روش فلس‌برداری
        ابوالفضل علی وردی داود عسگری
        سوسن از گل­های مهم در صنعت گل و گیاهان زینتی می­باشد. این گل از دسته گیاهان سوخواره بوده که با استفاده از سوخ کشت می­گردد. کشت فلس از سریع­ ترین و اقتصادی­ترین روش­های ازدیاد سوخ لاله در بین سایر روش­ها به شمار می­رود. تولیدکنندگان سوخ گل More
        سوسن از گل­های مهم در صنعت گل و گیاهان زینتی می­باشد. این گل از دسته گیاهان سوخواره بوده که با استفاده از سوخ کشت می­گردد. کشت فلس از سریع­ ترین و اقتصادی­ترین روش­های ازدیاد سوخ لاله در بین سایر روش­ها به شمار می­رود. تولیدکنندگان سوخ گل سوسن همواره با مشکلات متفاوتی در طی مراحل مختلف تولید مواجه می­شوند. پژوهش حاضر با هدف حل مشکلات مرحله اول ازدیاد سوخ از طریق فلس­برداری با دو آزمایش مجزا طراحی و اجرا گردید. در آزمایش اول چهار تیمار از دو نوع اکسین (ایندول- 3 - بوتیریک اسید و ایندول نفتالین استیک اسید در غلظت­های 250 و 500 میلی­گرم در لیتر) به همراه تیمار شاهد به­عنوان فاکتور اول و سه بستر کشت (کوکوپیت، کوکوپیت+پرلیت و پیت ماس سیاه) به­عنوان فاکتور دوم بررسی شدند. نتایج نشان داد بالاترین تعداد سوخک به ازای هر فلس (70/2) در تیمار 500 میلی­گرم در لیتر ایندول- 3 - بوتیریک اسید حاصل شد همچنین بالاترین مقدار وزن سوخک (81/0 گرم) و قطر سوخک (32/1 سانتیمتر) در تیمار شاهد حاصل گردید. نتایج بررسی اثر بسترهای کشت نشان داد بالاترین تعداد سوخک به ازای هر فلس (20/2)، وزن سوخک (71/0 گرم) و قطر سوخک (21/1 سانتیمتر) در بستر پیت ماس به­دست آمد. در آزمایش دوم، غلظت­های هورمونی فوق به­عنوان فاکتور اول و همچنین وجود یا عدم وجود نور به­عنوان فاکتور دوم مورد بررسی قرار گرفت. نتایج نشان داد وجود یا عدم وجود نور در بسیاری از تیمارهای ارزیابی شده به جز قطر سوخک معنی دار نبوده و تنها قطر سوخک در عدم وجود نور افزایش پیدا کرد. پژوهش حاضر نشان داد در ازدیاد سوخ سوسن با استفاده از هورمون­های گیاهی می­توان برخی از صفات کمی و کیفت محصول را بهبود بخشید. Manuscript profile
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        5 - Effect of Thidiazuron and Naphthalene Acetic Acid (NAA) on the Vase Life and Quality of Cut Alestroemeria hybrida
        H. Bagheri S. Sedaghathour
        Alstromeria is a new cut flower in Iran that due to the high performance, long life, and pretty flowers and colors diversity is highly regarded. Premature yellowing of leaves in cut flowersAlstromeria before petal fall is the most important factor limiting the vase life More
        Alstromeria is a new cut flower in Iran that due to the high performance, long life, and pretty flowers and colors diversity is highly regarded. Premature yellowing of leaves in cut flowersAlstromeria before petal fall is the most important factor limiting the vase life of flowers. To delay leaf yellowing and increased quality, factorial experiment based on completely randomized design carried out with the two factors thidiazuron (TDZ) (0, 10, 20 and 50 mg l-1) and naphthalene acetic acid (NAA) (0, 10 and 20 mg l-1). Results showed that the maximum vase life and the value of chlorophyll b were achieved (20 mg l-1 thidiazuron 20 mg l-1 NAA) and the most petals proteins was related to treatment 10 mg l-1 thidiazuron and 20 mg l-1 NAA. The most petals carotenoids and dry matter content and the least ethylene production was obtained in 10 mg l-1 TDZ and 10 mg l-1 NAA, respectively. Manuscript profile
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        6 - The effects of Cadmium nitrate and growth regulators in Phaseolus vulgaris L. tissue culture
        Aida Taghizadeh ghavidel Ahmad Majd masome mirzai
        Cadmium is a heavy metal that it’s poisonous forms can naturally exist in soil or can enter to soil in following ways:by industrial activities,mine probing and agricultural fertilizers.Phaseolus vulgaris L.is one of the plants that can tolerate poison of cadmium a More
        Cadmium is a heavy metal that it’s poisonous forms can naturally exist in soil or can enter to soil in following ways:by industrial activities,mine probing and agricultural fertilizers.Phaseolus vulgaris L.is one of the plants that can tolerate poison of cadmium and Bean is one of the most important species in Fabaceae family that have high economic significance in human nutrition.Regeneration by tissue culture is one of the effective and appropriate methods for preserving and also propagation of plants.In this study,research of effects cadmium nitrate and growth regulators in Phaseolus vulgaris L.tissue culture,percentage of germination,rhizogenesis and callus generation of seed of Bean plant cultivar(cos 16)in mediums containing cadmium nitrate and2ip hormone evaluated.In the Beginning seeds were surface sterilized respectively with:etanol70%,distilled water and sodium hypocholorite5%.Sterilized seeds were cultured into different culture containing:T0(MS),T1(MS+ 2ip: 0.1 mg⁄l );T2(MS + 2ip: 0.1mg⁄l +CdNo3:0.05mg⁄l);T3( MS+ CdNo3: 0.05mg⁄l)for percentage of germination.Explants of leaf and hypocotyl were prepared from ten-day sterilized seedling cultured in T0 medium and moved in T0(MS),T1(MS+2ip: 3mg⁄l );T2(MS+2ip: 5mg⁄l).A number of hypocotyl explants were transferred intoT1(MS+ NAA: 0.5mg⁄l),T2 (MS+ BAP:2.5mg⁄l) ;T3(MS+NAA:0.5mg⁄l+BAP:2.5mg⁄l). Eventually calluses of hypocotyl explant subcultured fromT0 medium into T0(MS), T1(MS+CdNo3:0.05mg⁄l);T2(MS+CdNo3: 0.1mg⁄l).According to the above results,maximum and minimum percentage of germination in T0(MS),T3(MS+CdNo3: 0.05mg⁄l).Best percentage of callus generation inT0(MS),was observed in hypocotyl explants.Also most rhizogenesis inT2(MS+2ip:5 mg⁄l)in leaf explant and Best callus generation and rhizogenesis were seen inT3(MS+NAA: 0.5 mg⁄l +BAP:2.5mg⁄l)in hypocotyl explant.Finally In subculture of the callus of hypocotyl explant,the most callus generation and rhizogenesis was observed in absence of cadmium nitrate:T0(MS). Manuscript profile
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        7 - The study of Yield and Yield Components of Red Beans in Condition of Drought Stress and Seed Pre-Treatment With Growth Regulators
        منا محتشمی احمد نادری علی اکبر قنبری مجتبی علوی فاضل شهرام لک
        In order to investigate the effect of seed priming with growth regulators on yield and yield components of red bean lines this research was conducted as spilt factorial in RCBD design with three replications. The treatment consists of three levels of irrigation (after e More
        In order to investigate the effect of seed priming with growth regulators on yield and yield components of red bean lines this research was conducted as spilt factorial in RCBD design with three replications. The treatment consists of three levels of irrigation (after evaporation of 60-55, 85-80 and 115-110 mm) two lines of red beans ( KS31169 ,D81083) and seed priming including salicylic acid and naphthalene acetic acid at four levels (P0:control, distilled water; P1: 0.5 m mol L-1 NAA; P2: 0.7 m mol L-1 SA; P3: combination of SA and NAA at rates of 0.5 and 0.7 m mol L-1). The results showed that the simple effects and the interactive effects of genotype × seed priming with on all the studied traits were significant except for the harvest index and pod length. Maximum 100-seed weight of (44 g) and biological yield of (14715.88 kg h-1 ) were achieved in the KS31169 line through the use of (NAA), under irrigation after 80-85 mm evaporation. The seed yield of (3454.16 kg h-1) were observed through the use of (NAA) and (SA) pretreatments for drought stress treatment. The results of stepwise regression of yield and the related traits 88 % of grain yield changes was explained by biological yield, grain yield, harvest index, number of seeds per pod and seed weight. Generally, it can be concluded that seed pretreatment can help improve and develop the yield under drought stress, directly and indirectly by affecting the morphological and physiological properties of plants. Manuscript profile
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        8 - Effects of Naphthalene Acetic Acid and Carbaryl on Fruit Thinning in ‘Kinnow’ Mandarin Trees
        Golnar Safaei-Nejad Ali-Reza Shahsavar Abbas Mirsoleimani
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        9 - Study of the effect of auxin and gibberellin on growth, total chlorophyll, carotenoid and terpenoid content in Euphorbia trigona Mill.
        Hakimeh  Rezaei Aryan Sateei Tahereh A.   Aghajanzadeh Mehdi Ebadi
        This study was conducted to investigate the effect of auxin and gibberellin on Euphorbia trigona Mill. in a randomized complete block design in 2020, and in greenhouse conditions. One minute pretreatments including auxin regulators NAA, IBA and IAA at a concentration of More
        This study was conducted to investigate the effect of auxin and gibberellin on Euphorbia trigona Mill. in a randomized complete block design in 2020, and in greenhouse conditions. One minute pretreatments including auxin regulators NAA, IBA and IAA at a concentration of 500 mg / l and treatments containing GA, IAA, IBA and NAA, 250 mg / l were compared with each other and with control plants. Stems and roots fresh weight, dry weight, dry weight percentage, total photosynthetic pigments content as well as total terpenoids were measured. Fresh and dry weight and the percentage of dry weight of stem did not show sensitivity to the treatments and pretreatments, and the effects were not significant. NAA treatment had the most positive effect on roots fresh and dry weight, although the percentage of root dry weight was not significantly affected by the treatments. The highest significant increase in terpenoid content was observed in NAA treatment and NAA and IBA pretreatments. GA treatment had the most positive effects on the content of each of chlorophylls a and b, total and carotenoid content. In terms of effect on photosynthetic antenna composition, the most positive effects on the ratio of total chlorophyll to carotenoids were observed in GA, NAA, IAA and IAA pretreatments. IAA treatment also showed a significant increase in chlorophyll a to b ratio. The correlation between growth parameters with each other and also the correlation of chlorophyll content with each other and with carotenoids was positive and significant. However, the correlation of terpenoid content with none of the other traits was significant. On the other hand and from an application point of view, based on gas chromatography-mass spectrometry measurements, naphthalene-acetic-acid treatment was effective in severely increasing the content of valuable medicinal compound, bis-2-ethylhexyl phthalate, compared to the control. Manuscript profile