List of Articles culture media

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  1 - Chemical composition of dill(Anethum graveolens L.) and parsley( Petroselinum sativum L.) essential oil in different culture media
  Mehrnaz Mahmoudi Zarandi Samiyeh Sistani
  10.30495/sarj.2023.1998375.1161
  The emphasis of the World Health Organization on the gradual replacement of natural materials instead of chemicals has caused different countries of the world to invest, and, plan mass production of medicinal plants at the industrial levels and use them in the pharmaceu More

  The emphasis of the World Health Organization on the gradual replacement of natural materials instead of chemicals has caused different countries of the world to invest, and, plan mass production of medicinal plants at the industrial levels and use them in the pharmaceutical, food and, health industries. Although the production of secondary metabolites depends on genetic factors, it is significantly influenced by environmental factors. The effects of different growth conditions (field and greenhouse) on the quantity and quality of essential oil of dill( Anethum graveolens L.) and parsley( Petroselinum sativum L.) were studied. Field cultivation was done on agricultural land in the Kerman Baqerabad region. In the greenhouse cultivation, germinated seeds were planted in pots with loamy soil( 2/3) and vermicompost (1/3) and, cocopeat and perlite. Rorison solution was used for feeding plants in pots. Plants were harvested after 3 months. Essential oils of 100 grams of dried herbs were isolated by water distillation using Clevenger apparatus. Essential oil analysis was done by gas chromatography-mass spectrometry (GC/ MS). According to the results obtained from the compounds identified in the essential oil of dill, the highest amounts of components were alpha-phellandrene (54.5%), phytol (18.65%) and apiol (49.2%) in the field, loamy-vermicompost and cocopeat-perlite media respectively. In parsley essential oil, myristicin was the highest in all three culture media. Manuscript profile
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  2 - The Effect of Using Native Carbon Sources (Whey, Oat bran, Rice Flour) in the Fermentative Production of Lysine by Corynebacterium glutamicum
  Zahra Mohammadzade Ferizhandi Ghazal Labbeiki
  Lysine is an essential amino acid that is not synthesized in the human body, but due to the body's need and its application in different parts of the health area, it is important to optimize and economize the production process. The most commonly used lysine production More

  Lysine is an essential amino acid that is not synthesized in the human body, but due to the body's need and its application in different parts of the health area, it is important to optimize and economize the production process. The most commonly used lysine production method is microbial fermentation and the nutrient composition of the fermentation media is effective on the microorganism growth and the production of microbial metabolite. Accordingly, the aim of this study was to investigate the effect of native and inexpensive nutritional substrates, as the main supplier of energy for microorganism, on the growth and productivity of strains. For this purpose, different amounts (g/lit) of whey (100, 135, 170), rice flour (100, 135,170) and oat bran (100, 170, 220) were separately added to culture of Corynebacterium glutamicum, and at 68 and 116 fermentation time, growth indices such as pH of the environment, production biomass, specific growth rate of microorganism, strain morphology and amount of produced amino acid were measured and compared with control (100 g/lit glucose). The results showed that the highest amount of lysine produced was obtained in media containing 135 g/lit whey, 170 g/lit rice flour and 220 g/lit oat bran. These results indicate that these compounds are suitable for use in biological processes and can be used as the main nutrient source. Manuscript profile
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  3 - Toxicity effects of alpha (α) and epsilon (ε) toxins produced by Clostridium perfringens type D
  Mahnaz Karimi Mehrdad Shamsaddini Bafti Majid Ezatkhah Mohammad Kargar
  Background and Objectives: Clostridium perfringens is one of the most pathogenic species in the clostridium genus. Alpha (α) and epsilon (ε) are the main toxins of this bacteria. This study aimed to express the genes encoding alpha and epsilon toxins of C. More

  Background and Objectives: Clostridium perfringens is one of the most pathogenic species in the clostridium genus. Alpha (α) and epsilon (ε) are the main toxins of this bacteria. This study aimed to express the genes encoding alpha and epsilon toxins of C. perfringens type D in two media (in the base medium and the base medium containing liver powder) and to analyze their toxicity. Materials and Methods: The standard strain was inoculated in the base enrichment medium, which half of them were enriched by powder liver. Then, RNAs were extracted and after converting to cDNA using double polymerase chain reaction, the gene expression was investigated on gels. The level of protein expression was measured by spectrophotometery and the cell toxicity of these proteins was determined by minimum lethal dose (MLD) assay. Results: The electrophoresis of PCR products showed two bands, 324 and 625, which represented successful expression of the genes encoding of alpha and epsilon toxins in both media. The mean cell cytotoxicity of the proteins expressed in the base medium and the base medium containing liver powder were measured 1/4000 and 1/4333, respectively. The mean protein production in the base medium and the base medium containing liver powder were measured 94.39 and 48.01 mg/ml. Conclusions: This study showed that these genes are expressed in the presence of liver powder. Furthermore, based on the MLD assay in these media, although these genes expressed the genes in the presence of liver powder, this additives did not had any effects on their toxicity. Manuscript profile
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  4 - The Effect of Calcium Intake on Adverse Effects of Salinity of Vermicompost in Production Cucumber Transplant (Cucumis Sativus)
  Kazem Alikhajeh Mohammad Khajeh-Hosseini
  Salinity, especially in arid and semi-arid regions, is one of the major agricultural issues around the world. Vermicompost has high levels of potassium, phosphorus and sodium, which leads to increased salinity. High salinity is a limiting factor in the consumption of th More

  Salinity, especially in arid and semi-arid regions, is one of the major agricultural issues around the world. Vermicompost has high levels of potassium, phosphorus and sodium, which leads to increased salinity. High salinity is a limiting factor in the consumption of this substance. Calcium ions have significant effects on plant physiological processes and improves the morphological and biochemical parameters of plants subjected to salinity stress (NaCl). To investigate the effect of calcium on the improvement of damage caused by vermicompost salinity as the experiment was conducted as a completely randomized design in autumn 2014 in a research greenhouse of Faculty of Agriculture, Ferdowsi University of Mashhad. In this experiment, calcium nitrate (99%) was used as a calcium source. The treatments consisted of calcium vermicompost control, 10mM calcium treated before seeding, 25mM calcium treatment and 75mM calcium treatment with culture medium. The results of this experiment showed that calcium presence had a significant effect on salinity effects and 10mM calcium had the most positive effects on the growth characteristics of cucumber in vermicompost culture medium. The proper application of calcium ion reduces the sensitivity of plants to salinity and improves the desired parameters, therefore, the use of crops with high calcium application efficiency can help reduce economic costs against salinity. Manuscript profile