List of Articles banana wastes

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  1 - Extraction and characterization of tyrosinase from banana wasted
  Ali reza farrokh reyhane sariri nikoo nasoohi
  Tyrosinase-Michaelis Constants, Optimum Temperature and pH-assisted formation of brownish pigment spots on peel is one of the most important factors in making signs of aging. It also has a direct effect on reducing the quality of fruits and vegetables through producing More

  Tyrosinase-Michaelis Constants, Optimum Temperature and pH-assisted formation of brownish pigment spots on peel is one of the most important factors in making signs of aging. It also has a direct effect on reducing the quality of fruits and vegetables through producing melanin. Tyrosinase is a metalloenzyme that is used in research works on foodstuffs, medicines and cosmetics. Edible mushrooms are currently the only source of tyrosinase for the industrial and research units using this enzyme. This study aims to evaluate tyrosinase from a banana peel as an alternative to tyrosinase from mushrooms in the pharmaceutical, food, cosmetic and research industries. In practice, the enzyme extracted from banana peel was purified and some of its properties, including biological activity, pH and optimum temperature, were determined and compared with tyrosinase of other proteins. The results indicated that the tyrosinase enzyme of the banana peel has two isoforms with molecular weight more than the tyrosinase of mushrooms. On the other hand, the temperature and pH of the enzyme were 50 and 6.1˚ C, respectively. The Michaelis–Menten kinetics and the maximal rate of velocity (Vmax) were also estimated to be 263.3 mM (m/mol) and 0.00401 mmol/min, respectively. Key Words: Extraction, Banana Wastes, Tyrosinase, Manuscript profile
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  2 - Study Kalamin D and Benzocaien on Tirosinas enzyme activity by Lineviverberg
  Ali reza farrokh reyhane sariri Nikoo Nasoohi
  Tyrosinase, the enzyme responsible for biosynthesis of melani pigments has a special place. It is a metaloenzyme containing copper ions that catalyses two distinct reactions in the pathway of melanin biosynthesis. The monophenolase activity leads to hydroxylation of phe More

  Tyrosinase, the enzyme responsible for biosynthesis of melani pigments has a special place. It is a metaloenzyme containing copper ions that catalyses two distinct reactions in the pathway of melanin biosynthesis. The monophenolase activity leads to hydroxylation of phenylalanin and the oxidation of 3, 4 dihydroxy phenylalanin to O-dopaquinon is through its diphenolase activity. Inhibitors and activators of the enzyme has a wide variety of effects on human health as well as in industrial applications. Considering the importance of pigments in human skin and hair and the role of tyrosinase in the production of melanin pigments, in the present research three important skin medications were selected for their effect on tyrosinase activity. In practical section, the enzyme was first extracted from banana skin followed by its characterization. The kinetic parameters were then investigated in the presence and absence of benzocaein, zinc oxide and kojic acid and the Michaelis Menton as well as Linweaver Burk plots were obtained. The results indicated that tyrosinase extracted from banana skin is similar to mushroom tyrosinase and its optimum temperature is higher than known tyrosinases. Therefore, by bearing in mind its low priced source, it can be recommended as an alternative to the commercial enzyme from mushroom. The results also showed that all three medicines were un-competitive inhibitors of tyrosinase. They reduced the maximum rate, Vmax of enzymatic reaction with no effect on Km. The highest inhibitory activity was obtained for zinc oxide Keywords: Tyrosinase, banana wastes, benzocaein, kojic acid, zinc oxide Manuscript profile