List of Articles Zizyphus jujuba seeds

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  1 - آپوپتوز ناشی از اسانس دانه عناب در سلول های سرطانی کولورکتال لاین HT-29
  زهرا زمانی الهام قدوسی دهنوی رضا حاجی حسینی محمد ارجمند سیما نصری
  10.30495/medherb.2021.686381
  Background & Aim: In traditional medicine, Zizyphus jujuba (jujube) has been used due to its medicinal properties and various physiological functions such as antioxidant, anticancer and anti-inflammatory properties. This study was conducted to study anti-cancer and More

  Background & Aim: In traditional medicine, Zizyphus jujuba (jujube) has been used due to its medicinal properties and various physiological functions such as antioxidant, anticancer and anti-inflammatory properties. This study was conducted to study anti-cancer and apoptotic effects of essential oil from Zizyphus jujuba seeds and to evaluate the effect of essential oils on p53, APC and KRAS genes expression in HT-29 colorectal cell line.Experimental: In this study, the essential oil of jujube seeds collected from orchards in Isfahan was prepared by Clevenger apparatus and then analyzed by GC-MS. The effects of toxicity and cell viability were determined using Trypan Blue, MTT and clonogenic methods. DNA fragmentation and apoptosis techniques were used to evaluate the mechanism of the effects of essential oil on cell death by flow cytometry. Finally, Real-time PCR was used to evaluate the expression of p53, APC and KRAS genes and their role in induction of apoptosis.Results: Essential oil of Zizyphus jujuba seeds reduced the viability of cells by concentration and time-dependent manner as compared to the control group. The essential oil also induced apoptosis by increasing gene expression p53 and suppressing gene expression KRAS.Recommended applications/industries: The present results indicated that essential oil of Zizyphus jujuba seeds induces apoptosis by targeting genes involved in colon carcinogenesis. However, further investigations on signaling pathways are needed to fully confirm the results of this study. Manuscript profile
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  2 - بررسی تغییرات هماتولوژیک و ایمونولوژیک ناشی از مصرف جلبک اسپیرولینا در مقایسه با داروی لوامیزول در سگ های نژاد پرشین شفرد
  سام ترکان محمد جواد عکاشه
  10.30495/medherb.2021.686382
  Background & Aim: Spirulina microalgae is known to has beneficial effects in the treatment of many animals diseases. Effects of Spirulina platensis microalgae, singly or in comparison with levamisole drug are not still studied in dogs. Thus, the present study was co More

  Background & Aim: Spirulina microalgae is known to has beneficial effects in the treatment of many animals diseases. Effects of Spirulina platensis microalgae, singly or in comparison with levamisole drug are not still studied in dogs. Thus, the present study was conducted to investigate the effects of this microalgae on hematological and immunological parameters in dogs.Experimental: In this research, 24 native breed dogs were used for 30 days. The dogs were divided into three groups of eight randomly, including a control group, treatment group (received 2 g Spirulina orally) and the third group received 5­mg/kg Levamisole. At the end of mentioned period, hematocrit percent, Hb, RBC, WBC, Neutrophil, Lymphocyte, Eosinophil, monocyte, basophil levels and immunological factors including IgM, total protein and phagocytic precent were measured.Results: Hematocrit in levamisole receiving group increased significantly (P<0.05). Results showed that RBC and Hb levels in Spirulina receiving group were significantly higher than the control and levamisole groups. Alb and Neutrophil levels in Spirulina receiving group were lower than the other two groups. In addition, Eosinophil and WBC levels in control group were significantly lower than the other two groups, but monocyte and Lymphocyte levels in control group increased significantly (P<0.001). Also, on the 30th day, total protein and phagocytic percent in Spirulina receiving group were significantly higher than others groups. In addition, in Spirulina and levamisole groups a significant increase in IgM level was observed (P<0.001).Recommended applications/industries: It can be concluded that the use of Spirulina in dogs for a short time could improve hematological and immunological factors and the effects of  Spirulina alga in the most factors is comparable to levamisole drug. Manuscript profile