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      • Open Access Article

        1 - The effect Vitex Agnus-Castus on serum concentration of cortisol, progesterone and luteinizing hormone in dairy cows
        Farhoodi Moghadam, M., Khalafi, S.M.A. .
        In this study a 50 ml hydroalcoholic extract of the plant, Vitex-agnus prescribed to cyclic Holstein cows orally, and its effects on serum Cortisol, Progesterone and Luteinizing hormone concentrations were assessed. Cows divided into control (n=7) and treatment (n=7) gr More
        In this study a 50 ml hydroalcoholic extract of the plant, Vitex-agnus prescribed to cyclic Holstein cows orally, and its effects on serum Cortisol, Progesterone and Luteinizing hormone concentrations were assessed. Cows divided into control (n=7) and treatment (n=7) groups. After 21 days’ prescription of the extract in treatment group, both groups synchronized by two intra muscular injection of PGF2α in 11 days apart. Prescription of herbal extract of Vitex agnus-castus in the treatment group continued till to end of synchronized cycle. During this estrous cycle blood samples collected and ovaries and uterus of cows were examined ultrasonographically. After 53 days’ treatment, progesterone, LH and cortisol concentrations of serums that were taken in days, 5, 9, 13 and 18 of estrous cycle measured by ELISA kit. Statistical analyses were done by SPSS. An analysis of repeated measures with 95% Confidence Interval was carried out by (GLM) procedure for times (days) trends measures. An independent t test was done for comparison of the data. The data of treatment group before and after treatment was analysed by paired t test. Results showed that Vitex-agnus increases serum progesterone concentration versus before treatment and control group (P>0.05). The cortisol concentration after treatment decreased but it was not significant. The LH concentration in treatment group only in days 13 increased (not significantly). These results show that the herbal extract probably, have positive effects on progesterone, LH blood concentration increase and cortisol blood concentration decrease in Holstein cows. Manuscript profile
      • Open Access Article

        2 - Comparison of antimicrobioal effect of essential oil of Vitex agnus – castus with common antibiotics in vitro.
        mohammad dakhili Hamideh Afarin محمدرضا ذوالفقاری
        Vitex agnus – castus (VAC) as a plant in herbal medicine has been since centuries ago. It is a phytoestrogenic herb native to the Middle East and southern Europe. It has clinical usage in so many countries. In this research, the antimicrobial effect of essential o More
        Vitex agnus – castus (VAC) as a plant in herbal medicine has been since centuries ago. It is a phytoestrogenic herb native to the Middle East and southern Europe. It has clinical usage in so many countries. In this research, the antimicrobial effect of essential oil of VAC was investigated on Staphyloccocus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and was compared with Ampicilin, Gentamicin, Nystatin.Essential Oil of VAC was extracted by steam distillation, Chemical constituents was analyzed by gas chromatography-mass spectrometry. for evaluation of antimicrobial effect Disk diffusion and Macrodilution methods were used.Essentical oil of VAC inhibited the growth of Staphylococcus aureus and Candid albicans at Concentrations 56/25 and 112/5 respectively. by Disk diffusion method The inhibitory zones were 10 mm and 11 mm respectively This essential oil inhibited the growth of Pseudomonas aeruginosa and Escherichia coli at concentration 450 . by Disk diffusion method They didn’t show inhibitory zone.The results of this research indicated that the essential oil of VAC has antimicrobial effect, in comparison with antibacterial effect of Ampicilin and Gentamicin has significant difference (P<0/001) antifungal effect of this essential oil in comparsion with Nystatin doesn’t have significant difference (P>0/05). The majer constituents of oil were: Terpinolene, Caryophyllene, α-pinene. Manuscript profile