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      • Open Access Article

        1 - Capacity of nettle (Urtica Dioica L.) in adsorption of heavy metals (Pb, As, Cd, Ni) from Tonekabon landfill leachate
        Khashayar Sharifi Aptin Rahnavard keivan saeb Farid Gholamreza Fahimi Ahmad Tavana
        Background and Objective: Landfill leachate contains heavy metals that cause toxic effects on water and soil near landfills. Phytoremediation is one of the control methods in which native plants with accumulation ability are used. The aim of this study was to evaluate t More
        Background and Objective: Landfill leachate contains heavy metals that cause toxic effects on water and soil near landfills. Phytoremediation is one of the control methods in which native plants with accumulation ability are used. The aim of this study was to evaluate the accumulation strength of heavy metals by nettle from leachate. Material and Methodology: In the present study, after collecting nettle seeds from Tonekabon landfill, they were planted in 16 pots and after the 6-leaf stage, placed under four concentrations of fresh leachate (0, 30, 60 and 100%) and after the growth period, the amount of metals Heavy (by atomic absorption spectrometer) and morphological traits were measured. Findings: The result showed that with increasing leachate concentration, the amount of heavy metals in all organs increased (Ni> Pb > Cd> Ar) and the aerial parts were more absorbed. In parallel with this result, dry weight factors of stem and leaf, root and leaf area index also decreased. Calculation of TF> 1 in nettle organs showed its ability to be more accumulative in the face of leachate. Discussion and Conclusion: Bioremediation with using of native plants and accumulators to remove heavy metals is a low cost and environmentally friendly method. Nettle is a native and wild plant that grows in landfills in the north of the country and due to its accumulative power, it is a suitable species for clearing the soil of heavy metals. Manuscript profile
      • Open Access Article

        2 - Triterpenes from Urtica dioica L. roots of Ethiopian origin
        Tegene Tole
      • Open Access Article

        3 - Antimicrobial and Healing Effect of Nettle, Purslane and Hedge Nettle Extracts on Burn Infections of Staphylococcus aureus in Mice
        Nader Kazemi Mahdi Arfaei Mona Ghasemi
        Introduction & Objective: Staphylococcus aureus is cause of hospital infections and infectious illnesses. Urtica dioica, Portulaca oleracea and Stachys schtschegleevii have more antimicrobial and healing effects. In this project, the antimicrobial and healing effect More
        Introduction & Objective: Staphylococcus aureus is cause of hospital infections and infectious illnesses. Urtica dioica, Portulaca oleracea and Stachys schtschegleevii have more antimicrobial and healing effects. In this project, the antimicrobial and healing effects of plants extracts with silver sulfadiazine were studied on burn infections of Staphylococcus aureus in rats. Materials & Methods: Firstly, ethanolic and acetonic extracts of plants were prepared in the laboratory. Then, the MIC and MBC of the extracts were determined by the dilution method in the Muller Hinton broth. In study of animal model, firstly the bacteria were inoculated with a concentration of (5×105 CFU/ml) to the wound site on rats. After 24 hours, ointments were prepared based on MBC concentration from extracts of mentioned plants for 1g of silver sulfadiazine and was used to treatment.Results: In studies conducted on rats, it was found that ethanolic and acetonic extracts of Urtica dioica, as well as the acetonic extract of Portulaca oleracea had more antimicrobial and healing effect on Staphylococcus aureus. But in the wound treated with ethanolic and acetonic extract of Stachys schtschegleevii, bleeding was seen. Conclusions: Ethanolic and acetonic extracts of Urtica dioica and the acetonic extract of Portulaca oleracea had more antimicrobial and restoration effects on burn wound infection. Ethanolic and acetonic extract of Urtica dioica had better healing effects than acetonic extract of Portulaca oleracea. As a result, extract of Urtica dioica could be used in preparation of burn ointments.  Manuscript profile
      • Open Access Article

        4 - Effect of nettle extract on black bean aphid (Aphis fabae (Scopoli)), wheat aphid (Schizaphis graminum (Rondani)) and cowpea aphid (Aphis craccivora (Koch)) in comparison with soap compounds
        Kamal Ahmadi A. koushki H. M. Takaloo zadeh
        Aphids are among the most important economic pests and vectors of many plant pathogens in horticultural, agricultural and greenhouse crops in world. In this study, the effect of ethanolic extract of nettle Urtica dioica L. (Rosales: Urticaceae), Palizin® soap and Fi More
        Aphids are among the most important economic pests and vectors of many plant pathogens in horticultural, agricultural and greenhouse crops in world. In this study, the effect of ethanolic extract of nettle Urtica dioica L. (Rosales: Urticaceae), Palizin® soap and Firooz® baby shampoo on three species of aphids (black alfalfa aphid, Aphis fabae (Scopoli), wheat poison aphid, Schizaphis graminum (Rondani), and cowpea aphid, Aphis craccivora (Koch)) were examined. Several experiments included evaluation of the birth percentage of adult insects, total mortality and mortality of 5-6- and 1-2-day-old nymphs and also adult insects treated with the above compounds. Hosts of these pests in all steps of this study were included wheat plants for poisonous wheat aphids, alfalfa for black alfalfa aphids and beans for black bean aphids. The results of this study showed that ethanolic extract of nettle has a significant effect on pest mortality. The result showed the ethanolic extract of nettle has a significant effect with the means ± SE percentage of mortality (90.50±3.76) and (87.78±3.92) in wheat poison aphid, (74.00±9.30) and (79.00±5.76) in black alfalfa aphid and (62.00±9.20) and (64.50±4.56) in cowpea aphid on 1-2-and 5-6- day-old nymphs, respectively, in comparing to control. So, according to the results, ethanolic extract of nettle has a significant effect on the control of these pests and it can have capacities that suggest conducting further studies in the management of Aphids.   Manuscript profile
      • Open Access Article

        5 - Repellency of camelthorn (Alhagi camelorum F.) and nettle (Urtica dioica L.) extracts against Tribolium castaneum (Coleoptera: Tenebrionidae)
        F. Shakhsi Zare S. Loni F. Moradi L. Azizpour
        The insert repellency of camelthorn (Alhagi camelirum F.) and nettle (Urtica dioica L.) extracts was investigated against red flour beetle (Tribolium castaneum) adults. The plants They were dried in room temperature and soaked in 98% ethanol, then concentrated by use of More
        The insert repellency of camelthorn (Alhagi camelirum F.) and nettle (Urtica dioica L.) extracts was investigated against red flour beetle (Tribolium castaneum) adults. The plants They were dried in room temperature and soaked in 98% ethanol, then concentrated by use of evaporation apparatus. Extract concentrations of 5, 10, 30 and 50% were prepared with pure acetone. Experiments were carried out according to filter paper method. Repellency percentage of each extract was recorded after one, three, five and ten hours of exposure at four replicates. The results showed that, repellency of both extracts increased significantly as concentrations rate and time of intervals increased. The highest repellency (76.6%) achieved 10h after exposure for camethorn and the least (3.33%) was obtained after one hour of treatment for nettle. Also, the repellency of camelthorn was more than nettle in all concentrations. According to results of this study, the camelthorn has a good potential to use as repellant for control of stored product pests. Manuscript profile
      • Open Access Article

        6 - Study the toxicity effect of Urtica dioica stem and leaf extract on tobacco aphid Myzus nicotianae
        Marzieh Shazdeahmadi
        Tobacco aphid (Myzus nicotianae) is one of the most important pests of tobacco and reduces the quality and quantity the tobacco product, directly and indirectly. Today, chemical insecticides are used to control pests. Due to the fact that plant extracts have a short she More
        Tobacco aphid (Myzus nicotianae) is one of the most important pests of tobacco and reduces the quality and quantity the tobacco product, directly and indirectly. Today, chemical insecticides are used to control pests. Due to the fact that plant extracts have a short shelf life in the environment and have low toxicity to humans, mammals and other living organisms, they are a good alternative to the side effects of chemical insecticides. In this study, the insecticidal properties of nettle Urtica dioica leaf and stem extracts on tobacco aphids were investigated. The experiments were performed at a temperature of 25 ±5° C, a relative humidity of 60±5% and a photoperiod of 16L: 8D. After extracting the aqueous extract of nettle by soaking method, for bioassay experiments, the 15 number of adults tobacco aphids were placed on filter paper and the lethal effect of mortality concentrations up to one percent in four replications on tobacco aphids were investigated. The lethal effect of these two extracts at 5 different concentrations (0.5, 1, 5, 10 and 15 mL/L) in four replications on the percentage of tobacco aphid mortality was investigated. The 50% lethal concentration (LC50) was calculated using the Probit program. The results showed that nettle leaf extract with (LC50 = 3.08 mL / L) showed more toxicity than nettle stem extract with (LC50 = 5.11 mL /L). The application of nettle leaf extract with a concentration of 15 mL / L after 48 hours caused 100% mortality on tobacco aphids, which is significantly higher than nettle stem extract (with 90% mortality). Overall, the results of this study showed that the extracts of both plant organs, especially nettle leaf extract, have good power to control tobacco aphids. Manuscript profile
      • Open Access Article

        7 - Protective effects of nettle (Urtica dioica) extract against acute kidney injury induced by gentamycin in the rat
        mahdieh hajjavadi akram eidi سید پژمان Mortazavi
        Aminoglycosides are often used in combination with beta-lactam antibiotics and have a rapid bactericidal effect, are available at an affordable cost and have less incidence of resistance, making them a drug of choice for treatment of several life-threatening infections. More
        Aminoglycosides are often used in combination with beta-lactam antibiotics and have a rapid bactericidal effect, are available at an affordable cost and have less incidence of resistance, making them a drug of choice for treatment of several life-threatening infections. However, the nephrotoxic effects of aminoglycosides prevent their long term use. The use of herbal extracts in order to decrease injuries of injurious materials has long been considered. The present study was conducted in order to investigate the protective effects of nettle (Urtica dioica) extract against gentamicin induced kidney injuries in the rat. Forty five male Wistar rats were divided into 9 groups consisting of: 1-healthy control group, 2- negative control group that received tween 20 (extract solvent), 3- patient control group which received onlygentamicin at 100 mg/kg, experimental healthy groups 4-6 which received nettle extract at 50, 100 and 200 mg/kg and patient experimental groups 7-9 which received nettle extract along with gentamicin at 100 mg/kg. At the end of the experiment (28 days), blood samples were obtained, and the kidneys were removed for histopathologic investigations. The results showed that gentamicin alone induced renal tissue damage and significantly increased the serum levels of creatinine and urea (p<0.05). However, administration of nettle extract accompanied with gentamicin decreased these markers significantly (p<0.05). Also histologic results indicated improvement of renal tissue structures during treatment with nettle extract.  It is concluded that nettle could ameliorate nephrotoxicity induced by gentamicin.   Manuscript profile
      • Open Access Article

        8 - Some effects of different habitats on antimicrobial activities of (Urtica dioica L.)
        elham mogheyseh مه لقا Ghorbanli,
        Different habitats don’t have the same effects on the growth of plants and different climates can influence the rate of efficient compounds of plants. So, we make a decision for researching about Urtica dioica L. that it’s a medicinal plant and in traditiona More
        Different habitats don’t have the same effects on the growth of plants and different climates can influence the rate of efficient compounds of plants. So, we make a decision for researching about Urtica dioica L. that it’s a medicinal plant and in traditional medicine, it has been used for cure of many diseases. During the reproductive period of stinging nettle the sample of this plant was collected from four regions of Gorgan city situated in Golestan province. The ethanol extract of different organs of U. dioica L. was extracted and effected on three bacteries, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans, by using MIC and MBC method. The results of this study showed that ethanol extract has antibacterial and antifungal activity. Prepared extract from high altitude had more antimicrobial effects. It was clear that leaf had most efficient extract among these four evaluated organg (MIC: 62.5mg/ml) and in most cases, stem extract didn’t have antimicrobial effect or its effect was little. But E. coli excluded of this rule and its root extract always was the most efficient extract in all of the altitudes and had the best inhibitory and fatality (MIC 7.8 mg/ml, MBC 15.62 (mg/ml) effects. Manuscript profile
      • Open Access Article

        9 - The Effect of Urtica dioica Hydro-Alcoholic Extract on the Plasma Level of Pituitary Thyroid Axis Hormones and Some of the Liver Enzymes in Adult Male Wistar Rat
        حیدر آقابابا سیده نیره حسینی سید ابراهیم حسینی
        Urtica dioicais a herbaceous, perennial and enumerate medicinal plant.Urtica dioica is an indigene plant in Iran and in last years many pharmaceutics effect are reported about it plant. In this research, the effect of Urtica dioicahydro-alcoholic extract of the plasma l More
        Urtica dioicais a herbaceous, perennial and enumerate medicinal plant.Urtica dioica is an indigene plant in Iran and in last years many pharmaceutics effect are reported about it plant. In this research, the effect of Urtica dioicahydro-alcoholic extract of the plasma levels of thyroid hormones (T4, T3), TSH and liver enzymes,alanin amino transfrase(ALT), aspartate amino transfrase(AST), alkaline phosphatase(ALP) were studied. In this experimental study, the experiments were performed on 40 adult male wistar rats with weight about 210andplusmn;10 gr, which were divided into 5 groups. The experimental groups received 25, 50 and 100 mg/kg of the Urtica dioica extract with injected intraperitoneally (IP) for 28 days respectively. Sham group received only solvent (distilled water) and control group did not receive any thing. At the end of experiment by collecting the blood samples from heart animals, plasma level of T3,T4 and TSH were measured by using ELISA Technique and enzymes liver AST, ALT, ALP by photometric method. Detected data were analyzed by T-test and ANOVA (pandle; 0/05). The results showed significant increase in the level of TSH hormone in the each three experimental groups compared with the control group. The level of T4 hormone significantly decreased in the each three experimental groups compared with the control group. Also the level of T3 showed significant decrease in experimental group receiving 50,100 mg/kg extract compared with the control group. The Level of AST, ALT enzymes in experimental groups showed no significant difference compared with the control group, While ALP enzyme in experimental and sham groups showed significant increase compared with control group. The results of this research have been shown that extract of this plant could affect on HPT axis and have no effect on some of the liver enzymes respectively. Manuscript profile