List of Articles Sphingomonas

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  1 - Isolation and molecular identification of n-hexadecan degrading bacteria from compost
  Mohammad Reza Samaei Seyed Bagher Mortazavi Bita Bakhshi Ahmad Jonidi Jafari Mina Bostanshenas
  Background and Objectives: Contamination of soil and groundwater by diesel released from underground storage tanks is an important and extensive environmental challenge in Iran. The aim of this study was to isolate and molecular identification of n-hexadecane-degrading More

  Background and Objectives: Contamination of soil and groundwater by diesel released from underground storage tanks is an important and extensive environmental challenge in Iran. The aim of this study was to isolate and molecular identification of n-hexadecane-degrading bacteria from compost. Materials and Methods: In this study, hexadecane was used as a model for contamination to diesel oil. We used compost in order to isolate hexadecane degrading bacteria. Then, the selected bacteria was identified using PCR method. Finally, the efficiency of isolated bacteria for consumption of hexadecane as sole source of carbon and the growth rate of the bacteria in different concentrations of NaCl were evaluated. Results: Based on morphology, biochemical tests and sequencing, the isolated bacteria identified as Sphingomonas yanoikuyae. This bacteria could remove 49.69% of n- Hexadecane after 33 days in 30 C. The concentration of n- Hexadecane decreased from 3000 mg to 1510 mg. In addition, the results showed that isolated bacteria can growth on 2.5% salinity. Conclusion: The results of this study showed that S. yanoikuyae can be used in remediation of petroleum products, especially diesel oil, in tropical and semi-salinity area in Iran. Therefore, further investigations using molecular approaches is recommended. Manuscript profile
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  2 - Screening and characterization of novel methane oxidizing bacterial strains from oil contaminated soils in Khuzestan, Iran
  Nazanin Sanei Mohammad Roayaei Ardakani Mohammadreza Soudi
  Background & objectives:Based on information about the types and numbers of microorganisms in the surface soil samples, the distribution range of underlying oil and gas reservoirs can be judged. In the present study, methane oxidizing bacteria were isolated and char More

  Background & objectives:Based on information about the types and numbers of microorganisms in the surface soil samples, the distribution range of underlying oil and gas reservoirs can be judged. In the present study, methane oxidizing bacteria were isolated and characterized from oil contaminated soils in Khuzestan, Iran and their growth was optimized in the presence of methane as sole carbon and energy sources. Materials & Methods: Bacteria were isolated in nitrate mineral salt (NMS) medium in the presence of 50% air and 50% methane. The cultures were incubated on a shaker for 10 days at 30°C in dark place, and every two days, the gas mixture was replaced. The isolated bacteria were characterized based on biochemical and molecular identification tests. Then the optimum growth conditions was detected in different pH values and incubation temperatures in NMS medium containing methane. Results:Three Gram-negative rods were isolated from soil samples that were able to grow in isolation condition. The isolates were characterized as Achromobacter and Sphingomonas spp. The strains could also grow in the NMS medium with a high methanol concentration (3%). The optimal pH and temperature for the isolates were 7.4 and 30°C respectively. Conclusion: Methanotrophic strains that were isolated in the present study were able to grow and oxidize methane in high ranges of temperature and pH and can be proposed for the removal of mono-carbon compounds such as methane and as biological detectors for prospecting for oil and gas reservoirs Manuscript profile