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        1 - Effect of the ionic liquid 1-butyl-3-methyl-imidazolium bromide as adjuvant on the formation of {PEG 600-potassium citrate} aqueous biphasic system at different temperatures
        Sholeh Hamzehzadeh Mostafa Abbasi
        Abstract: In biotechnology, separation, extraction, and purification of biomolecules using aqueous biphasic systems, as environmental and economic sustainable alternatives for conventional water-organic solvent extraction techniques, have always been the focus of great More
        Abstract: In biotechnology, separation, extraction, and purification of biomolecules using aqueous biphasic systems, as environmental and economic sustainable alternatives for conventional water-organic solvent extraction techniques, have always been the focus of great attention and examina-tion. One approach proposed by researchers is based on the use of ionic liquids (ILs) as adjuvants in ABS, making the capability of these systems for the extraction of biomolecules to be promoted. In this regard, this work is devoted to study the effect of IL 1-butyl-3-methyl imidazolium bromide ([C4C1im] Br) on the formation of ABS Composed of a polyethylene glycol (PEG) with molecular weight 600 and a biodegradable organic salt potassium citrate. For this purpose, the binodal curves and the liquid-liquid equilibrium (LLE) data of the studied ABS, along with the partition coeffi-cients of [C4C1im]Br were determined at two temperatures of 278.15 K and 318.15 K. The results obtained indicate that the ability of [C4C1im]Br to promote the formation of the studied ABS de-creases with increasing temperature, so that at 318.15 K, the addition of IL makes the formation of two-phase system more difficult. In addition, [C4C1im]Br displays the partition coefficients greater than one for all the compositions and temperatures studied, which increases with increasing TLL at a given temperature. Manuscript profile