List of Articles PCR method

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Antimicrobial effect of Carvacrol on Aspergillus flavus and reduce expression of aflR gene in the aflatoxin biosynthetic pathway
  Seyed Amir Ali Anvar Hamed Ahari Nasim Mojarrad
  Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Evaluation of CCND1 and ITGB1 genes expression in patients with breast cancer
  Farnam Gholipour Maralan shohreh Zare Karizi Mohammad Ebrahim zadeh
  Breast cancer is the most common type of invasive cancer and the second cause of cancer-related death in women. Therefore, evaluating the molecular mechanisms is of crucial importance in this respect. the CCND1 gene encodes the Cyclin D1 protein and Cyclin D1 overexpres More

  Breast cancer is the most common type of invasive cancer and the second cause of cancer-related death in women. Therefore, evaluating the molecular mechanisms is of crucial importance in this respect. the CCND1 gene encodes the Cyclin D1 protein and Cyclin D1 overexpression has been shown to correlate with early cancer onset and tumor progression. Cyclin D1 overexpression can also down-regulate Fas expression, leading to increased chemotherapeutic resistance and protection from apoptosis. In addition, Integrin beta-1 (ITGB1), is a cell surface receptor that in humans is encoded by the ITGB1 gene. Abnormal levels of integrin beta-1D have been found in limb girdle muscular dystrophy and polyneuropathy.Sample from tissues of 30 patients with breast cancer and the normal margin of tissues were bought from Imam Khomeini hospital. Afterwards, genomic RNA extraction and cDNA synthesis using quantitative real-time PCR method was implemented and after primer design for CCND1 and ITGB1 genes, gene expression was analyzed. The CCND1 and ITGB1 genes demonstrated a significant expression increase in breast cancer tumor samples compared to adjacent normal samples (P <0.0001). The increased expression of CCND1 and ITGB1 genes may enhance the process of cell cycle and this condition can develop tumorigenesis; however more research is needed to confirm these findings. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Identification of virulence genes in serotype O:3 Yersinia enterocolitica isolated from turkey meat by PCR method in Shahrekord
  Atieh Hadad elahe tajbakhsh Maryam Reiysi
  Nowadays are being used PCR-based methods in order to detect Yersinia enterocolitica strains and plasmids and chromosomal virulence genes. This method able to provide a rapid and reliable characterization. In this study, 300 samples of turkey meat were collected randoml More

  Nowadays are being used PCR-based methods in order to detect Yersinia enterocolitica strains and plasmids and chromosomal virulence genes. This method able to provide a rapid and reliable characterization. In this study, 300 samples of turkey meat were collected randomly from market in Shahrekord. The isolates were identified using PCR test, And the virulence genes of the isolates were detected. According to the results, 55 serotypes O:3 were identified, and the prevalence of virulence genes were as follows: yadA (32.72%), inv (100%), ail (38.18%), ystA (40%), and virF (27.27%). Due to the increased consumption of turkey meat in the country and the possibility of Y. enterocolitica transfer to humans, it is recommended to carefully observe storage principles, avoid use of undercooked turkey meat and also applying PCR test for direct identification of contamination in food as a quick, accurate and specific technique is recommended. Key words: PCR method, Turkey meat, Virulence genes, Yersinia enterocolitica Manuscript profile