Cheese isamong ther dairy products and is a suitable environment for the growth and reproduction of microorganisms. One of these pathogens, Neospora caninum, causes abortion in cows. This study aimed to investigate the prevalence of N. caninum in traditio More
Cheese isamong ther dairy products and is a suitable environment for the growth and reproduction of microorganisms. One of these pathogens, Neospora caninum, causes abortion in cows. This study aimed to investigate the prevalence of N. caninum in traditional cheeses produced from ruminant milk using molecular method. In this study, 86 traditional cheese samples consisting 42 cow cheese, 20 sheep cheese, 12 goat cheese, and 12 sheep cheese ripened in brine were randomly collected from dairy herds in Chahar Mahal and Bakhtiari provinces. The samples were transferred to the laboratory in sterile conditions next to ice an, and Nested-PCR technique was used on Nc5 gene for molecular analysis. The results showed that 9.3% (8/86) of the samples were contaminated with N. caninum. The highest contamination (12.5%) was found in cow milk cheese samples. No contamination was observed in traditional cheeses produced from goat milk or sheep brined cheese. The comparison of contamination levels in different cheeses showed no significant difference between the groups. Since milk and dairy products have a high place in the human food basket; therefore, the healthiness of these foods is crucial.
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Background and Objectives: Neospora caninum is one of main etiologic factors of abortion in in cattle. The mass production of protozoa in laboratory conditions is performed based on growing in different cell lines. Since using suspension cell lines are very cost e More
Background and Objectives: Neospora caninum is one of main etiologic factors of abortion in in cattle. The mass production of protozoa in laboratory conditions is performed based on growing in different cell lines. Since using suspension cell lines are very cost effective for mass production of biological products, this study aimed to evaluate the suspension cell culturing for production of this protozoan.
Materials and Methods: This study was experimentally performed bases on growth of N. caninum tachyzoites on Ka6 cell line (a cell line obtained from cattle infected with Theileria lestoquardi, Razi Institute, Shiraz, Iran. Next, based on MTT assay, ability of this cell culture for production of N. caninum was compared with Vero cell as the best current cell line for this purpose.
Results: The results showed that N. caninum tachyzoites could enter into K6a cell lines and after replication released from the cells successfully. Replication of the tachyzoites was significant in both Vero and K6a cell lines in compare to the control.
Conclusion: To our knowledge, this is the first and successful report of suspension culture of N. caninum tachyzoites. Although, the rate of N. caninum proliferation on Ka6 cell line did not show any significant difference in compare to Vero cell line, since Ka6 cell line is a transformed lymphocytic cell and it is possible to grow massively this cell line as suspension bioreactors, it is preferred to Vero cell line.
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The protozoan Neospora caninum is known worldwide as one of the most important causes of abortion storms in cattles, especially when dogs living in the cattle farms. Currently, the most consideration is on live attenuated vaccines. The main goal of this research is to e More
The protozoan Neospora caninum is known worldwide as one of the most important causes of abortion storms in cattles, especially when dogs living in the cattle farms. Currently, the most consideration is on live attenuated vaccines. The main goal of this research is to evaluated live-attenuated Neuspora caninum (NC-a) strain as an effective vaccine to prevent oocyst shedding in dogs. Sixteen dogs were randomly divided into four equal groups including live attenuated strain without adjuvant, acute strains, live attenuated strain with Montanide adjuvant and control group. All the groups were immunized twice, two weeks apart. One month after the second injection, all dogs were challenged orally by acute NC-1 strain. Immune responses were assay by agglutination test and molecular test was used to assess oocyte shedding. The results showed that the immunized group with live attenuated strain with adjuvant produces the highest immune response. Molecular test also showed that the immune response in immunized dogs prevented oocyst shedding after challenge and the best result is in the group of dogs immunized by live attenuated strain with adjuvant. Overall, the results of this study showed that research on the live attenuated Neospora caninum vaccine with oily adjuvants in dogs is secure and effective in creating an immune response and controlling oocyte shedding. In conclusion, this experimental vaccine can be used as a promosing vaccine in towards researches to obtain a succesful vaccine to control neosprosis in dogs.
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