List of Articles Mycoplasma bovis

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Molecular epidemiology of isolated Mycoplasma bovis strains from clinical mastitis infections in Cattle
  Imandar, M., Pourbakhsh, S.A.*, Jamshidian, M., ZahraeiSalehi, T. .
  Mycoplasma bovis is one of the main pathogenic species and the most common cause of pneumonia, mastitis and arthritis in cattle. The aim of this study was to evaluate the epidemiological factors (age, level of production,herd size, type of discharge, drop in p More

  Mycoplasma bovis is one of the main pathogenic species and the most common cause of pneumonia, mastitis and arthritis in cattle. The aim of this study was to evaluate the epidemiological factors (age, level of production,herd size, type of discharge, drop in production and a history of clinical mastitis) in isolated Mycoplasma bovis strains from clinical mastitis infection in cattle. Samples from 328 cows with clinical mastitis were taken by purposive samplingmethod, and were sent with ice and up to 24 hours to Mycoplasma reference laboratory, Razi Vaccine and Serum Institute of Karaj. Isolation and identification by culture and PCR methods was done, Mycoplasma bovis was isolated and 31 isolates were confirmed by nucleotide sequencing method. One of the isolates had 100 percent genetic relationship with reference strain of Mycoplasma bovis PG45 ATCC25523. Also, one of the isolates was different in nucleotide sequence with other isolates and the rest had 99.7% homology. Most positive cases were present in the age group 4-6 years and 800 cattle herd size and in this sense there was a statistically significant difference between samples (P<0.05). In terms of production and consequent drop in production mastitis infection, there was no significant difference between positive samples (P>0.05). The results showed that the mammary secretions could not be definitivediagnosis of this type of mastitis. Also, more positive samples had one of mastitis in their history. The results showed the presence of Mycoplasma bovis in milk samples is significant in clinical mastitis of cows in Iran and principles of biosecurity and quarantine should be at the top of Mycoplasma bovis infection control programs. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Identification of Mycoplasma bovis in cows with clinical mastitis using culture and Polymerase Chain Reaction based on 16SrRNA and uvrC genes
  Mohsen Imandar Seyed Ali Pourbakhsh Mahmood Jamshidian Taghi zahraei salehi
  Mycoplasma bovis is one of the main causes of pneumonia, mastitis and arthritis in cattle. The aim of this study was to identify the M. bovis in cows with clinical mastitis using culture and PCR based on 16SrRNA and uvrC genes. A total of 328 milk samples were obtained More

  Mycoplasma bovis is one of the main causes of pneumonia, mastitis and arthritis in cattle. The aim of this study was to identify the M. bovis in cows with clinical mastitis using culture and PCR based on 16SrRNA and uvrC genes. A total of 328 milk samples were obtained from cattle with clinical mastitis in industrial dairy herds. Samples were sent to the Mycoplasma reference laboratory, Razi Vaccine and Serum Research Institute at 4°C in less than 24 hr and incubated for 18-24 hr at 37°C. After filtering to specific PPLO medium, they were incubated in the presence of Co2 and were monitored for 7-10 days. Simultaneously, DNA of the samples was extracted and purified by Phenol-Chloroform method and PCR was used to detect mycoplasma genus based on 16SrRNA and detection of M. bovis species based on uvrC genes. Out of 328 samples, 58 samples were positive in PPLO agar media. Ninty-seven samples (29.57%) were positive for mycoplasma gene, of which 14 samples (4.26%) showed specific gene of M. bovis. The results of this study introduced the PCR method as a rapid and reliable test for detection of M. bovis in milk specimens. The results also showed that the presence of M. bovis in dairy herds in Iran is high and it can be considered as one of the main causes of the clinical manifestation of cattle mastitis. Manuscript profile