List of Articles Multiplex- PCR

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  1 - Comparison of PCR and histopathology methods for diagnosis of Yersiniosis infection (yersinia ruckeri) in Rainbow trout of Iran
  عادل Haghighi Khiabanian Asl M.R Roozbahani بهرام Kazemi
  Yersinia ruckeri is the causative agent of Yersiniosis or Enteric Red mouth Disease that causeshuge amount of economic loss in mariculture industry worldwide. Definitive detection andeffective treatment for septicemia like Y. ruckeri that outbreak critically, requires r More

  Yersinia ruckeri is the causative agent of Yersiniosis or Enteric Red mouth Disease that causeshuge amount of economic loss in mariculture industry worldwide. Definitive detection andeffective treatment for septicemia like Y. ruckeri that outbreak critically, requires rapid andspecific diagnosis. Despite the advantages of both PCR and Histopathology techniques in theterms of diagnosis and monitoring they have some defects, the aim of this study is thecomparison of PCR and histopathology methods for detection of Yersiniosis in trout fish inIran.In PCR-based detection, Extracted DNA from suspected Rainbow trout tissue utilized inPCR reaction and polymerase chain products were visualized by gel electrophoresis. Inpathological detection, all tissues from live or moribund fish were fixed in 10% formalin saline.Then Samples were embedded in paraffin block and sectioned with digital microtome at 5-7micrometer thickness. Slides were stained by haematoxillin & eosin, and then treated withmounting media. Differences in the number of positive samples in this comparison demonstratedthat each mentioned technique has its advantages so if the advantages of each method exploitand one method supplements the other one, the efficiency and accuracy of monitoring theexperiments would boost. In both mentioned methods, all positive results confirmed theexistence of yersiniosis infection in Iran, which their comparison is very useful for monitoringand diagnosis of pathogen in the country. Differences in the number of positive samples in thiscomparison demonstrate that although PCR technique would provide a more rapid and sensitivealternative to traditional diagnosis in detection of harmful pathogens but in some cases such asnecrotic and degenerated cells in tissue, PCR ability collapses in the comparison of otherdetection technique like histopathology. In this study, 20 suspected samples were tested by bothtechniques that 2 samples were positive and 18 samples were negative in PCR, and 5 sampleswere positive and 15 samples were negative in pathologic technique . Manuscript profile
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  2 - Determining the presence of virulence genes Panton Valentine leukocidin PVL and methicillin gene mecA in Staphylococcus aureus
  کیومرث امینی سجاد علیزاده
  Staphylococcus aureus is a major cause of food poisoning in the world that is created by consumption of contaminated food. Resistance to a variety of common and specific antibiotics is increasing. Staphylococcus aureus including PVL and gene mecAto heat pasteurization a More

  Staphylococcus aureus is a major cause of food poisoning in the world that is created by consumption of contaminated food. Resistance to a variety of common and specific antibiotics is increasing. Staphylococcus aureus including PVL and gene mecAto heat pasteurization and many proteolytic enzymes are stable and can remain active for a long time in food samples. The purpose of this study was to isolate Staphylococcus aureus and identify virulence gene PVL and gene of methicillin resistance in food samples by multiplex PCR technique has been used. The study included 120 samples of various foods (dairy, confectionery, meat and vegetables) collected 40 cases (33/3%) Staphylococcus aureus isolates were detected. Antibiotic susceptibility testing by disk diffusion method according to CLSI guidelines was conducted. To identify and confirm Staphylococcus aureus virulence and resistance genes from multiple PCR assay was used. Antibiogram results showed that antibiotics are among the most sensitive to the antibiotic vancomycin, Teicoplanin and methicillin respectively 95%, 90% and 75%. Resistance to linezolid, azithromycin and methicillin respectively, 35%, 32% and 25% more than other antibiotics was tested. Prevalence of methicillin resistance gene mecAin total 57.5% and PVL gene was not detected. Also 16srRNA gene in all samples was identified genus and species and confirmed. Different distribution of methicillin resistance gene in this study with other studies showing the potential risk of methicillin resistant Staphylococcus aureus in the world. Therefore, early detection and timely resistant strains, in order to prevent the spread of resistance appears to be necessary. Manuscript profile