List of Articles Microbial resistance

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  1 - Survey Genotyping of Animal and Human ‏ ‏‏Klebsiella pneumoniae‎ Isolates using ERIC-PCR and evaluation ‎ of Antibiotic Sensitivity Pattern
  Estabraghi, E., Zahraei Salehi, T.*, Amini, K., Jamshidian, M. .
  Klebsiella pneumoniae, is an opportunistic pathogens and cause infections in humans and animals. Drug resistant K. pneumoniae is rising. Therefore, antimicrobial susceptibility testing before prescribing antibiotics, it seems necessary. The aim of present study was to s More

  Klebsiella pneumoniae, is an opportunistic pathogens and cause infections in humans and animals. Drug resistant K. pneumoniae is rising. Therefore, antimicrobial susceptibility testing before prescribing antibiotics, it seems necessary. The aim of present study was to survey typing of clinical and animal K. pneumoniae isolates and evaluation of antibiotic susceptibility. A total 100 clinical and animal K. pneumoniae isolates were collected from Babak city. Antibiotic susceptibility was performed with Kirby-Bauer method according to CLSI guidelines. Then, DNA genomic extraction was done using DNA kit and PCR amplification was performed with ERIC1 and ERIC2 primer. Our results were shown that all strains (100%) were resistant to the ampicillin and amikacin antibiotics. The most and least resistance belong to tetracycline (53 strains; 88.3%) and imipenem (8 isolates; 13.3%), respectively. The results of cluster analysis and drawing dendrogram based on genetic similarities for 100 isolates was separated to seventeen distinct groups. According to our finding indicated an increasing resistance to antibiotics amongst K. pneumoniae. Additionally, the ERIC sequences have a pair of games that contain highly reversed and central reps and are located in the outermost regions of the bacterial genome and have less complexity in determining the genetic diversity of all isolates, but the separation good at the strain level.     Manuscript profile
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  2 - In vitro Effect of Carotenoid Pigment of Rhodotorula glutinis on Staphylococcus Aureus Isolated from Bovine Mastitis
  S. Naisi, منصور Bayat, , T Zahraei Salehi, بهاره Rahimian Zarif, R. Yahyaraeyat,
  10.30495/jcp.2022.20937
  Mastitis, as a most costly disease of dairy cows, causes a lot of damage to the livestock industry in the world every year. One of the most important bacteria which causes clinical mastitis in cattle is S. aureus, which has become resistant to most common antibiotics in More

  Mastitis, as a most costly disease of dairy cows, causes a lot of damage to the livestock industry in the world every year. One of the most important bacteria which causes clinical mastitis in cattle is S. aureus, which has become resistant to most common antibiotics in veterinary medicine. Therefore, researchers are looking for new methods in the treatment of such infections, in which the pigments of microorganisms fall into this category. The aim of this recent study was to optimize the production of carotenoid pigments by Rhododorula yeast and to investigate its antimicrobial effect on S. aureus isolates To isolate S. aureus, samples were taken from the milk of 100 cows with mastitis and the femA gene was identified for molecular confirmation among suspicious isolates of Staphylococcus by PCR method. Also, the isolate of R. glutinis obtained from one of the samples of mastitis was further examined and used as a sample of yeast producing pigment. The results showed that in addition to S. aureus as one of the causative agents of mastitis in dairy cattle, but also the yeast R. glutinis can be a causative agent of mastitis. The inhibitory effect on the growth of Staphylococcus using the pigment extracted from R. glutinis was also quite evident and more than 80% of S. aureus isolates were sensitive to the pigment at a concentration of 200 μg. R. glutenis inhibits excellent growth in S. aureus as one of the leading causes of mastitis in cows. The cause is the appearance of synthetic antibiotics and the reduction of microbial resistance. Manuscript profile
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  3 - Identification of the Bacterial agents of urinary tract infections in children in the Qom city in 1389
  محمد دخیلی
  AbstractObjective: Urinary tract infection is most common disease of reproductive system and the second of the urinary bacterial infection in children. These study investigated the bacterial causes, symptoms and antibiotic resistance in children with urinary tract infec More

  AbstractObjective: Urinary tract infection is most common disease of reproductive system and the second of the urinary bacterial infection in children. These study investigated the bacterial causes, symptoms and antibiotic resistance in children with urinary tract infections have been done.Methods: This study was performed as descriptive cross-sectional one and 70 children attending to Khorrami Hospital in Qom Province were evaluated for microbiological findings and antibiogram.Results: The most common germ was E. Coli in 7/55 percent. The antimicrobial resistance pattern was similar for fluorocinolones, cephalosporines, and aminoglycosides with other antibiotics. The intermittent sensitivity was not seen; in 38.6% and the resistance was highest to cephalosporines.Conclusions: Totally, according to the obtained results and comparison with other studies, it may be concluded that prevalence of anti-microbial resistance among children with UTI is relatively high and some sterategies should be developed to reduce it and its complications.Key words: Anti-microbial resistance, Children, UTI Manuscript profile
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  4 - Prevalence and antimicrobial resistance of Listeria species isolated from filleted Argyrosomus hololepidotus, Scomberomorus commerson and Alburnus spp.
  Ebrahim Rahimi Mohammad Javad Jahanmard Sohrab safari Mahsa Ansari Zeinab Torki baghbadorani
  Listeriosis is one of the most important food-borne diseases caused by Listeria species, especially L. monocytogenes.  The objective of this study was to determine the prevalence and antimicrobial resistance of Listeria species isolated from three types of fish fil More

  Listeriosis is one of the most important food-borne diseases caused by Listeria species, especially L. monocytogenes.  The objective of this study was to determine the prevalence and antimicrobial resistance of Listeria species isolated from three types of fish filet in Isfahan and Bandaranzali. From August 2009 to April 2011, a total of 120 samples of Argyrosomus hololepidotus (n= 90), Scomberomorus commerson (n=80) and Alburnus spp (n=70). Fish were obtained from randomly selected retail stores in Isfahan and Bandaranzali cities and were evaluated for the presence of Listeria spp. using standard cultural and PCR methods. Then antibiogram tests were carried out for determination of antimicrobial resistance. Seven (8.8%) and 6 (15%) of smoked and salted fish samples were positive for Listeria spp. respectively. L. monocytogenes, L. innocua and L. seeligari were isolated from 2.5, 6.7 and 1.6% of fish samples. Overall, 9 of 13 Listeria isolates (69.2%) were resistant to one or more antimicrobial agents. Resistance to tetracycline (53.8%) and tetracycline (30.8%) were the most common finding. All of the isolates were susceptible to gentamicin, vancomycin and chloramphenicol. The results of this study indicate the potential risk of infection with Listeria in people consuming raw or undercooked smoked and salted fish. Also, the results obtained in this study indicated the need for appropriate surveillance and epidemiological monitoring strategy to control the development of resistance.  Manuscript profile
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  5 - شناسایی مواد موثره موجود در عصاره و اسانس گیاه زینتی-دارویی سداب (Ruta graveolens L) و بررسی اثر ضد میکروبی آن‌ها
  فاطمه ترابی داود هاشم آبادی بهزاد کاویانی لیلا اسدپور
  این پژوهش با هدف بررسی مواد موثره موجود در عصاره و اسانس گیاه زینتی-دارویی سداب و بررسی اثر ضد میکروبی آن‌ها انجام شد. بدین‌منظور در آبان ماه 1398 اندام هوایی سداب از زیستگاه طبیعی آن در ارتفاعات چابکسر-استان گیلان جمع‌آوری شد. برگ‌ها و ساقه گیاه در آون الکتریکی با دمای More

  این پژوهش با هدف بررسی مواد موثره موجود در عصاره و اسانس گیاه زینتی-دارویی سداب و بررسی اثر ضد میکروبی آن‌ها انجام شد. بدین‌منظور در آبان ماه 1398 اندام هوایی سداب از زیستگاه طبیعی آن در ارتفاعات چابکسر-استان گیلان جمع‌آوری شد. برگ‌ها و ساقه گیاه در آون الکتریکی با دمای 75 درجه سانتی‌گراد به مدت 24 ساعت خشک و سپس توسط کلونجر عصاره‌ و اسانس گیاه استخراج شد. جهت شناسایی ترکیبات موثره عصاره و اسانس از GC-MS استفاده شد. نتایج نشان داد که Hexadecanoic acid، Isomaturnin، 2-Ethyl-1,3,4,5,6,7,8-heptamethy و12-methoxy-19-norpodocarpa به‌ترتیب بیشترین ترکیب موجود در اسانس برگ، اسانس ساقه، عصاره برگ و عصاره ساقه سداب است. نتایج تست میکروبی به روش دیسک دیفیوژن روی سویه‌های Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli نشان داد که عصاره برگ و ساقه سداب بیشترین اثر را در برابر مهار باکتری‌ A. baumannii دارد. بررسی نتایج MIC و MBC نشان داد که باکتری S. aureus با کمترین MIC و MBC حساس‌ترین باکتری در برابر اسانس برگ و ساقه سداب است درحالی‌که A. baumannii با کمترین MBC بیشترین حساسیت را به عصاره برگ سداب نشان داد. بنابراین می‌توان از عصاره و اسانس گیاه سداب به-عنوان یک ماده ضد میکروبی علیه باکتری‌های بیماری‌زای انسانی استفاده نمود. Manuscript profile
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  6 - The predatory potential of Bdellovibrio bacteriovorus against clinically isolated pathogens with extensively drug-resistance (XDR)
  Salman Odooli Rasoul Roghanian Giti Emtiazi Milad Mohkam Younes Ghasemi
  Background & Objectives: Bdellovibrio-and-like organisms (BALOs) are a group of predatory bacteria which invade other Gram-negative bacterial cells for growth. The bacteriolytic nature of Bdellovibrios make them one of the promising alternatives for conventional ant More

  Background & Objectives: Bdellovibrio-and-like organisms (BALOs) are a group of predatory bacteria which invade other Gram-negative bacterial cells for growth. The bacteriolytic nature of Bdellovibrios make them one of the promising alternatives for conventional antibiotics. In this study, the isolation and molecular identification of Bdellovibrio bacteriovorus strain SOIR-1 was described. The antibiotic resistance pattern of some clinically isolated Gram-negative pathogens was determined, and the predatory potency of SOIR-1 toward them was evaluated. Material & Methods: Double-layer agar technique, transmission electron microscopy, and PCR targeting the Bdellovibrios-specific hit locus were used for the isolation, morphological investigation, and molecular identification of SOIR-1, respectively. Following the antibiotic resistance profile determination of clinical isolates, the bacteriolytic activity of SOIR-1 against them was evaluated through the plaque formation assay and lysis analysis in the broth co-cultures. Results: SOIR-1 was identified as a strain of Bdellovibrios bacteriovorus through the transmission electron microscopy examination and specific PCR detection. All clinical isolates showed the properties of extensively drug-resistant (XDR) and typical Bdellovibrios plaques were developed on their lawns of cells. The SOIR-1 had the highest and lowest predation efficiency among the clinical isolates toward Acinetobacter baumannii (84.33%) and Pseudomonas aeruginosa-369 (55.16%), respectively. Conclusion: This study highlights the great potential of SOIR-1 to prey and lyse XDR pathogens, regardless of their antimicrobial resistance state. So, B. bacteriovorus can be considered as a living antibiotic in the cases of infections caused by multidrug-resistant bacteria. Manuscript profile
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  7 - Assesment of Antimicrobial Resistance Modes in Children with Pyelonephritis
  Hulal Saleh Sahib Falah Mahdi Al-Khafaji Ahmed Ali Obaid
  10.22034/jchr.2022.690409
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