Lily (Lilium spp.) from Liliaceae familiy is considered as one of the most important ornamental plants with high economic value. Virus diseases are shown to be as main limiting factor in lily production. During a survey in 2015, a total of 76 symptomatic samples showing More
Lily (Lilium spp.) from Liliaceae familiy is considered as one of the most important ornamental plants with high economic value. Virus diseases are shown to be as main limiting factor in lily production. During a survey in 2015, a total of 76 symptomatic samples showing mosaic, deformation, chlorosis, reduced growth, necrosis and decline were collected from lily growing greenhouses in Thehran province. Samples were tested for Cucumber mosaic virus (CMV) and Arabis mosaic virus (ArMV) using ELISA, biological and molecular methods. ELISA results showed that 45 and 3 samples were infected with CMV and ArMV, and 5 samples were co-infected with the both viruses. The virus infections were confirmed using biological inoculation tests. CMV and ArMV infection were also tested by RT-PCR method using specific primers which resulted to amplification of 1100 and 690 bp DNA fragments, respectively. CMV lily isolates were confirmed to belong to CMV subgroup I using specific primers in RT-PCR assay. Presence of Lily symptomless virus –LSV and Lily mottle virus-LMoV were tested by RT-PCR assay using specific primers which resulted in amplification of the expected 650 bp DNA product corresponding to CP gene region of LMoV, but there was not any DNA amplifications related to LSV. This is the first report on occurrence of ArMV, CMV and LMoV infections on lily in Iran. Detection of virus infection sources using the optimized methods in this study is a crucial practical approach for virus management in healthy lily bulb production in the country.
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کیفیت و کمیت گل‌های شاخه بریده لیلیوم تولید شده غالبا به اجزای بستر رشد بستگی دارد. جهت ارزیابی تأثیر دو منبع بستر پالم‌پیت (تنه خرما: آماده شده از تنه درخت خرما⸵ درخت خرما: آماده شده از تمام قسمت‌های درخت خرما) بر ویژگی‌های رشد گیاهی مربوط به گل شاخه بریده لیلیوم، یک آ More
کیفیت و کمیت گل‌های شاخه بریده لیلیوم تولید شده غالبا به اجزای بستر رشد بستگی دارد. جهت ارزیابی تأثیر دو منبع بستر پالم‌پیت (تنه خرما: آماده شده از تنه درخت خرما⸵ درخت خرما: آماده شده از تمام قسمت‌های درخت خرما) بر ویژگی‌های رشد گیاهی مربوط به گل شاخه بریده لیلیوم، یک آزمایش فاکتوریل بر پایه طرح کاملا تصادفی با سه تکرار برنامه ریزی گردید. در تحقیق حاضر، تأثیر یازده بستر رشد ریشه شامل مخلوطی از پالم‌پیت، کوکوپیت و پرلیت بر روی دو رقم (Tiber و Candy Club) تحت شرایط هیدروپونیک ارزیابی گردید. نتایج نشان داد که قطر ساقه، زمان ظهور گل، طول غنچه، ویژگی‌های محتواب آب نسبی برگ، نشت یونی برگ و گلبرگ، کیفیت بصری غنچه و شاخص کیفیت در رقم Candy Club بالاتر بود اما، ویژگی‌های SPAD1 و قطر غنچه در رقم Tiber بیشتر بود. بالاترین میزان کلروفیل شa، b و کل، SPAD1، SPAD3، قطر ساقه، قطر غنچه و شاخص کیفیت در تیمار شاهد (80% کوکوپیت + 20% پرلیت) مشاهده شد، که تفاوت معنی‌داری با برخی از تیمارهای بستر رشد تنه خرما و کوکوپیت مصرف شده ندارد. مقدار بالاتری از محتوای آب نسبی برگ، طول غنچه و کارتنوئید در تیمار 20% تنه خرما مشاهده گردید در‌حالی‌که، ماندگاری گل، در تیمار 60% درخت خرما بیشتر بود. تیمار 80% کوکوپیت مصرف شده بالاترین تعداد پیازچه، SPAD2 و زمان برداشت را نشان داد. کم‌ترین میزان نشت یونی برگ، زمان برداشت و زمان ظهور گل در تیمار شاهد مشاهده شد. ارقام مختلف به‌طور متفاوتی به بستر رشد پاسخ دادند و تنه خرما و کوکوپیت مصرف شده می‌توانند جایگزین بخشی، یا کل کوکوپیت گران‌قیمت وارداتی شوند. بسترهای تنه خرما برتر از بسترهای درخت خرما بودند، اما بستر شاهد برتری کمی نسبت به بسترهای تنه خرما داشت.
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Oriental hybrid lily “Arabian Red” mother scales were stored in moist cocopeat and perlite (1:1) for 3 or 5 months at 25 ̊C. The numbers of scale bulblets per mother scale were nearly identical in two durations. Increasing storage duration promoted the growt More
Oriental hybrid lily “Arabian Red” mother scales were stored in moist cocopeat and perlite (1:1) for 3 or 5 months at 25 ̊C. The numbers of scale bulblets per mother scale were nearly identical in two durations. Increasing storage duration promoted the growth of roots (number and length) and No. scales per scale bulblets. And, diameters of scale bulblets were more in longer storage duration (5 months) than 3 months. Therefore, storage duration affects size, no quantity of scale bulblets in “Arabian Red” lily. This can cause with giving opportunity to scale bulblet for more consuming the reserves of parent scale and extending the root growth, then produce greater bulblet. The results show that scale bulblets had more sucrose and glucose in 5 months, the content of fructose did not change in development process nevertheless, it seems, that has not any effect on the sprouting bulblets.
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In order to evaluat the effects of banzyladenine (BA)‚ gibberellic acid(GA3) and cold, dry storage on the longevity of liliumcut flowers (cv. Fangio) an experiment was conducted as a factorial based on randomized complete block design with three replications. Cut More
In order to evaluat the effects of banzyladenine (BA)‚ gibberellic acid(GA3) and cold, dry storage on the longevity of liliumcut flowers (cv. Fangio) an experiment was conducted as a factorial based on randomized complete block design with three replications. Cut flowers were treated with 100 mg/lit BA plus 100 mg/lit GA3 and distilled water (control) as a short-term pretreatment for 24 hours, then they kept in dry storage in 4ċ for 10 days. In dry storage, flowers were sprayed with 2 ppm nano silver or distilled water. After storage, cut flowers were placed in preservative solution containing 2 ppm nano silver plus 3% sucrose and were evaluated until the end of vase life. Results showed that addition of BA+GA3 (100mg/lit of each) as a pulsed treatment in solution for 24 hours prior cold storage increased petals anthocyanin, vase life (P<1%) and membrance stability index (P<5%) and decreased percentage of blasted buds and chlorophyllase activities. Stems kept in dry storage at 4ċ and then treated with preservative solution containing 2 ppm nano silver plus 3% sucrose produced the longest vase life (P<5%) and decreased percentage of blasted buds (P<5%).
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