List of Articles K562

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  1 - Exploring the effect of quercetin flavonoid encapsulated in chitosan nanoparticles on cytotoxicity, apoptosis, and growth of human cancer cell line
  روح الله Kamyabi علیرضا Jahandideh نگار Panahi صمد MohamamdNezhad
  10.30495/jcp.2023.22826
  Cancer is one of the most important causes of death in today's societies. Intolerable side effects and resistance to chemotherapy drugs have encouraged scientists to invent new drug compounds with less side effects. This study aimed to evaluate the synergistic effects o More

  Cancer is one of the most important causes of death in today's societies. Intolerable side effects and resistance to chemotherapy drugs have encouraged scientists to invent new drug compounds with less side effects. This study aimed to evaluate the synergistic effects of quercetin encapsulated in chitosan nanoparticles on cytotoxicity, apoptosis, and cell growth of K562 cell line. The flavonoid quercetin was encapsulated in chitosan nanoparticles and their physical characteristics were determined using standard methods, FTIR and SEM microscope images. K562 cells were cultured in cell culture medium, the cytotoxicity of drugs was evaluated by MTT method and the effects of nanodrugs on apoptosis in them were evaluated by flow cytometry. IC50 during 24- and 48-hours exposure of cells with nanoquercetin drug was determined as 16.71 and 10.36 μg/ml. Quercetin nanomedicine had a positive zeta potential. The data showed that the form of quercetin nanomedicine encapsulated with chitosan compared to the pure form of quercetin had a statistically significant effect on cancer cells (p<0.05). The results of the present study show that the encapsulated form of quercetin nanomedicine with chitosan has more cytotoxicity than the pure form of drugs. On the other hand, the results showed that quercetin in the form of a nano-drug complex had a significant effect in inducing apoptosis in human chronic myeloid leukemia cells. Therefore, this nanomedicine can be further investigated in the treatment of chronic myeloid leukemia. Manuscript profile
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  2 - Synthesis and Cytotoxic Evaluation of 2-Pyrazoline Derivative on Leukemia Cancer Cell Line K562
  Masoud Shaabanzadeh Maryam Bikhof Torbati
  10.22034/jchr.2018.545324
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  3 - Cytotoxic Effect of Seed Essential Oil of Zizyphus jujuba on K562 Leukemia Cell Line
  Elham Ghodousi Dehnavi H. Maghsoudi R. Haji Hosseini
  Essential oils are complex mixtures of several components endowed with a wide range of biological activities, including anticancer properties. Due to the side effects and the resistance of synthetic drugs, plant products are considered for the treatment of cancer. This More

  Essential oils are complex mixtures of several components endowed with a wide range of biological activities, including anticancer properties. Due to the side effects and the resistance of synthetic drugs, plant products are considered for the treatment of cancer. This study is performed the effect of cytotoxicity of essential oil from the seeds of Zizyphus jujuba (EOSZ) on K562 cell line as a model of chronic myeloid leukemia. In this study, the seeds of Z. jujube were collected from the Isfahan city, and essential oils were extracted by Clevenger apparatus and then the combination by gas chromatography and spectrophotometry were identified. K562 cells were cultured and treated with concentrations of essential oils of 1 to 900 micrograms per milliliter (in 24h, 48h, and 72h). Cytotoxicity of essential oil from the seeds of Zizyphus jujuba (EOSZ) against K562 leukemia cells was estimated by the MTT assay. The absorbance was measured using ELISA plate reader (540 nm) were measured. Then the data were analyzed with the computer software SPSS version 15.0 (SPSS Inc., Chicago, USA), t.test and ANOVA. 24 compounds were identified in the essential oil, the main component of which was Eugenol. Essential oil from the seeds of Zizyphus jujuba (EOSZ), the highest cytotoxic effect in LC50 = 200 micrograms per milliliter and 72 hours after treatment. In other words, the seed essential oil from Zizyphus jujuba (EOSZ) expresses the effects of cellular toxicity on k562 cells, depending on the dose and time. Considering the toxicity of seed essential oil from Zizyphus jujuba (EOSZ), seed essential oil from Zizyphus jujubacan be considered a Potential candidate for further CML studies, and other cancer cells. Manuscript profile