List of Articles Johne

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  1 - Identification of mycobacterium avium subsp paratuberculosis in samples tissue ileum of cattle slaughtered in Shahr-e Kord with ziehl neelsen staining and nested PCR method
  حسن کریمی عبدالرسول نامجو حسن ممتاز محمدرضا نامداری
  Johne s disease is a severe chronic progressive enteritis that infect digestive system ofruminants, domestic animals, wild animals and equine .This study performed due to distinguishM. avium paratuberculosis with nested polymerase chain reaction and Ziehl–Neelsen, More

  Johne s disease is a severe chronic progressive enteritis that infect digestive system ofruminants, domestic animals, wild animals and equine .This study performed due to distinguishM. avium paratuberculosis with nested polymerase chain reaction and Ziehl–Neelsen, s stainingon the ilieum samples of slaughtered cattle in Shahr-e- Kord industrial slaughterhouse. Resultsshowed that respectively 6.6% and 26.67 % of bovine are contaminated with M. aviumparatuberculosis according to histopathology method and nested polymerase chain reaction .Mac nemar statistical test showed that sensitivity of PCR test was more than histopathologicalmethod( P<0.001) . Also 80% total agreement between these two methods and the Kappacoefficients calculated at 0.33 which represent is poor agreement (P<0.01). There wasn’tsignificant deference between, gender, race and age with infection rate ( P>0.05). The resultsshowed that a nested PCR test for diagnosis of infection is high speed and accuracy and in theprimary levels of disease that amount of microorganism is a few we cannot trust to result ofhistopathological method for recognition of Johne‚s disease Manuscript profile
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  2 - Sero-prevalence of subclinical paratuberculosis (Johne's disease) in dairy farms of Tehran-Iran using absorbed ELISA assay
  Heidarnejhad, O., Safi, Sh., Mosavari, N., Keshavarz, R. .
  Mycobacterium avium subspecies paratuberculosis causes Johne's disease in ruminants and have been also isolated from humans. Accurate statistical information about the disease prevalence in different regions of Iran is not available. The objective of the present study w More

  Mycobacterium avium subspecies paratuberculosis causes Johne's disease in ruminants and have been also isolated from humans. Accurate statistical information about the disease prevalence in different regions of Iran is not available. The objective of the present study was to evaluate the prevalence of subclinical Johne's disease in dairy cattle herds of Tehran province, Iran.  Knowing the disease prevalence and its damages could have an important role to manage the disease in the farms involved. In the present study which was conducted in the years 1391 to 1393, 338 serum and fecal samples from cows older than 18 months, were obtained from 14 dairy farms in Tehran province, Iran. Antibodies against Johne's disease were assayed using PARACHEK2 absorbed ELISA kit (ParaCheck, Prionics AG, Zurich, Switzerland). Fecal culture and Nested-PCR assays were performed to confirm the disease. The prevalence of the disease determined by ELISA and culture were 9.5% (95% CI: 6.66 - 13.23) and 3.6% (95% CI: 1.69 - 7.27), respectively. Furthermore, the herd prevalence of the disease was calculated using culture as 28.6% (95% CI: 9.58- 57.99) and ELISA as 57.14% (95% CI: 29.65- 81.19). To evaluate the diagnostic value of the test, ELISA results were compared to bacterial fecal culture as the gold standard test. According to the obtained results, ELISA can be considered as a suitable screening test for the diagnosis of paratuberculosis due to the convenient sampling, high speed and low price. It is suggested that precise control programs to be performed based on the prevalence of the disease in Tehran province. Manuscript profile
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  3 - Comparison of culture and PCR methods for detection of Mycobacteriumavium subsp. paratuberculosis in raw milk of apparently healthy cattle
  یونس Anzabi
  Mycobacterium avium subsp. paratuberculosis is the etiological agent for Johne’s disease which is known as chronic disease in cattle and may attribute to Crohn’s disease in human. High prevalence of Mycobacterium avium subsp. paratuberculosis has been report More

  Mycobacterium avium subsp. paratuberculosis is the etiological agent for Johne’s disease which is known as chronic disease in cattle and may attribute to Crohn’s disease in human. High prevalence of Mycobacterium avium subsp. paratuberculosis has been reported in dairy cattle worldwide. Recognition of infected animals is a major factor to control the spread of the organism. In this regard, detection of the bacterium in milk of clinically suspicious and apparently healthy cows is the best way to control the infection. Although isolation of Mycobacterium avium subsp. paratuberculosis by culture assay is considered as the gold standard, PCR method helps us to recognize the occurrence of slow-growing microorganisms in a short period of time with high sensitivity. In this survey, a total number of 160 cow milk was sampled and cream layer together with the pellet of each sample was tested by PCR and culture technique. Using Kappa statistics it was revealed an almost perfectagreement between culture and PCR assay with a product size of 400 bp; however, the agreement between culture and PCR with product size of 228 bp was found substantial. Results showed a substantial agreement between PCR with product sizes of 400 bp and 228 bp. Comparing the agreement between the two PCR approaches with culture assay as gold standard test, it was assumed that PCR could be a robust and rapid method to detect Mycobacterium avium subsp. paratuberculosis in milk. Consequently, PCR can be introduced as a screening test for detection of the bacterium in cow milk. Manuscript profile
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  4 - Cloning of Mycobacterium avium paratuberculosis hsp70 gene into pET-24a plasmid and its expression in Escherichia coli
  Rasa Sheini mehrabzade Masoudreza Siefi abad shapori Masoud Ghorbanpoor Darush Gharibi
  10.30495/jvcp.2022.1954420.1362
  Johne's disease (Paratuberculosis) is a disease caused by Mycobacterium avium paratuberculosis (MAP) which is a chronic and progressive infection that chiefly affects ruminants. The disease can lead to significant economic losses in the livestock industry and may also b More

  Johne's disease (Paratuberculosis) is a disease caused by Mycobacterium avium paratuberculosis (MAP) which is a chronic and progressive infection that chiefly affects ruminants. The disease can lead to significant economic losses in the livestock industry and may also be a threat for public health; because it may be transmitted to humans through consumption of milk and other contaminated animal products. Immunity and resistance against the Johne's disease is mainly due to cell mediated immune response. Heat Shock Protein 70 (HSP70) of this bacterium is one of its important proteins, which the immune response against it can prevent the fecal excretion of bacteria. In order to facilitate the production of recombinant vaccine against Johne's disease, in this study the recombinant HSP (recombinant HSP; rHSP) was produced and its immunogenicity investigated in rabbits. For this purpose, the hsp70 gene was cloned into pET-24a plasmid and the resulting recombinant plasmid was transferred to E. coli strain BL21. The expression of the above protein was checked by SDS-PAGE and the accuracy of the nucleotide sequence was confirmed by sequencing. Immunization of rabbits by rHSP70 resulted in the production of high levels of antibodies. Based on the findings, it seems that the HSP70 specific antibody can be evaluated in the design of diagnostic methods of the disease and the produced recombinant protein can be assessed for the production of recombinant vaccines. Manuscript profile
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  5 - Evaluation of clinical and intestinal ultrasonographic findings in cows with Johne's disease
  mohammad tooloei gholamali moghaddam mohaddeseh fahimi
     Paratuberclosis or Johne’s disease is an infection of small intestine caused by Mycobacteriom avium paratuberculosis characterized by persistent and progressive diarrhea, weight loss, debilitation, and eventual death in cattle. A suitable and rapid me More

     Paratuberclosis or Johne’s disease is an infection of small intestine caused by Mycobacteriom avium paratuberculosis characterized by persistent and progressive diarrhea, weight loss, debilitation, and eventual death in cattle. A suitable and rapid method for diagnosis of the disease in farms is not available. Trans-abdominal ultrasound is a rapid, clinical and non-invasive diagnostic method recommended for diagnosis of animals with chronic inflammation of the intestine. The purpose of the present study was clinical and trans-abdominal ultrasound examination of cattle infected with organism of the Johns disease. This study was done on 20 female Holstein cattle with consist chronic diarrhea and/or weight loss. All animals were blindly tested for Mycobacteriom paratuberculosis antibodies in blood samples by ELISA and also examined by trans-abdominal ultrasound for intestinal wall thickness. The result of the ELISA test showed that 35% of the samples had antibodies against Mycobacteriom paratuberculosis. The rates of chronic diarrhea, low body condition score and both of them in cattle with positive ELISA were 100%, 71.4% and 71.4%, respectively. In the trans-abdominal ultrasound examination, the intestinal wall thickness of the infected animals (7.9±1.54 mm), was significantly (p<0.001) more than non- infected group (2.7±0.13 mm). This study indicates that the ultasonographic evaluation of the intestine and abdomen can be a quick, useful and on farm method with relatively high specificity in the diagnosis of cattle with John's disease. Manuscript profile
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  6 - Seroprevalence of Mycobacterium avium subspecies paratuberculosis infection in goats of Khuzestan province
  saeid Bagheri MAHDI POURMAHDI BORUJENI Mohammad rahim Haji Hajikolaei Masoud Ghorbanpoor
  Paratuberculosis or Johne's disease is chronic infectious granulomatous enteritis of ruminants, which has a worldwide occurrence. It is caused by Mycobacterium avium subspecies paratuberculosis and the most common symptoms of disease in goats are cachexia, anorexia and More

  Paratuberculosis or Johne's disease is chronic infectious granulomatous enteritis of ruminants, which has a worldwide occurrence. It is caused by Mycobacterium avium subspecies paratuberculosis and the most common symptoms of disease in goats are cachexia, anorexia and severe disability. The aim of this study was to survey seroprevalence of Mycobacterium avium paratuberculosis in goats of Khuzestan province and its correlation with host and environmental determinants. In this study sera samples were collected randomly from 368 goats in Ahvaz, Hendijan, Izeh, Shushtar, Dezful and Susangerd cities and were tested by ELISA. Apparent and real seroprevalence of Mycobacterium avium subspecies paratuberculosis were 7.07% (95% CI: 4.47-9.67 percent) and 13.8% (95% CI: 10.3-17.3 percent), respectively. This study showed that infection increased with age and odds of infection between the age, based on year and disease is 1.08 (95% CI: 0.86-1.35) (p>0.05). Relative frequency of positive samples in females was more than males and the odds of infection in female goats was 1.19 (95% CI: 0.4-3.59) times the males (p>0.05). The odds of infection in goats with history of diarrhea was 4.38 (95% CI: 1.92-9.96) times more than goats without this history (p<0.001). The seroprevalence in Ahvaz, Dezful, Izeh, Shushtar, Hendijan and Susangerd was 8.06% 5%, 4.92%, 13.33%, 2.67% and 5% respectively (p>0.05). This study confirms that Mycobacterium avium subspecies paratuberculosis exists in goats of Khuzestan province and preventive and control measures should be considered by health authorities. Conflict of interest: None declared. Manuscript profile
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  7 - Efficiency of direct microbial diagnosis, IS900 PCR and microbial culture for detection of Mycobacterium avium subsp. Paratuberculosis in the feces of apparently healthy cattle
  یونس Anzabi صمد Farashi Bonab Gh.A Moggaddam,
       Johne’s disease or paratuberculosis is a chronic granulomatous enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). The disease is responsible for significant economic losses in dairy industry worldwide.&nbsp More

       Johne’s disease or paratuberculosis is a chronic granulomatous enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). The disease is responsible for significant economic losses in dairy industry worldwide.  Microbial culture as a golden standard test for detection of MAP in faecal specimens requires 6-16 weeks to complete, whereas accurate and rapid identification of cattle shedding MAP in their feces is essential for successful control of the disease in herds. In the present study, direct microbial diagnosis by Ziehl-Neelsen acid-fast staining, microbial culture on Herrolds’ egg yolk media and two IS900 direct PCR assays were carried out on 100 fecal specimens of apparently healthy cattle collected from dairy herds of Tabriz with a history of Johne’s disease. The number of positive specimens identified by the direct microbial diagnosis, microbial culture and PCR with F90/F91 and FP25/FP26 primes were 7 (7%), 14 (14%), 15(15%) and 25(25%) respectively. These results indicated that PCR detected more positive cases therefore it can be employed for rapid and accurate diagnosis of cattle shedding MAP it their feces and the type of primer used has a significant role in the sensitivity of this test. Direct microbial diagnosis by Ziehl-Neelsen acid-fast staining identified 7 (7%) specimens, two IS900 direct PCR assays identified 15 (15%) and 25 (25%) specimens, respectively, and microbial culture identified 7 (7%) specimens as positive. Collectively, these data indicate that PCR detection of MAP was more sensitive than direct microbial diagnosis by Ziehl-Neelsen acid-fast staining or faecal culture, especially if appropriate primers were used. Manuscript profile
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  8 - Comarison of direct microscopic examination, Enzyme Linked Immunosorbent Assay (ELISA), culture and Nested-PCR for diagnosis of herds bulk tank milk infection with Mycobacterium avium subspecies paratuberculosis
  آریا Badiei فرهاد Mousakhani عباس Barin امیر Hamidi محسن Zafari
  Causative agent of Johne’s diseases is Mycobacterium avium subspecies paratuberculosis (MAP) and each year, it hurts dairy cattle industry worldwide by depression of production and reproductive indexes and culling of affected animals. This bacteria is known as a z More

  Causative agent of Johne’s diseases is Mycobacterium avium subspecies paratuberculosis (MAP) and each year, it hurts dairy cattle industry worldwide by depression of production and reproductive indexes and culling of affected animals. This bacteria is known as a zoonotic pathogen and recent researches explain its probable role in Crohn’s disease in humans. This study compared four laboratory diagnostic tests; including Enzyme Linked ImmunoSorbent Assay (ELISA), direct microscopic examination, milk culture and Nested-PCR for diagnosis of herds bulk tank milk infection with MAP bacteria. 100 bulk tank milk samples were obtained from 100 industrial dairy herds of Tehran province. Samples were screened by four tests and 82 samples (82%) detected as positive in culture media, 94 milk samples (94%) were positive by Nested-PCR test and 98 samples were positive in ELISA test results (98%). But direct microscopic examination identified just 33 milk samples as positive. Four positive samples of ELISA test were detected as negative samples by Nested-PCR. These 4 samples in addition to 12 other samples which diagnosed as positive by ELISA, had no growth in culture media (16 samples). These results indicated superiority of Nested-PCR among these four tests for diagnosis of bulk tank milk infection with MAP bacteria. Furthermore, the results represent high prevalence of MAP bacteria in bulk tank milk of dairy herds in Tehran province and it needs more serious eradication efforts.   Manuscript profile