Disturbance in wound healing process is considered to be one of the most serious complications of diabetes, because it significantly increases the susceptibility of patient wounds to be infected. Fenugreek is an annual plant which its leaves and seeds used extensively i More
Disturbance in wound healing process is considered to be one of the most serious complications of diabetes, because it significantly increases the susceptibility of patient wounds to be infected. Fenugreek is an annual plant which its leaves and seeds used extensively in foods and has advantages to human health. Due to prevalence rate and side effects of diabetes, Fenugreek seed extract is one of medicinal herb have been used more frequently. The present study was performed to investigate the effects of using fenugreek seed hydroethanolic extract on wound healing process in the 54 mice model of diabetes type 2. One single full-thickness excisional wound was created with 6-mm punch biopsy on back of each mice. All animal, accidentally, divided in three groups: Control group, 10% and 5% fenugreek seed hydroethanolic extract treatment groups. In terms of microscopic, the rate of tissue edema, neo-vascularization, fibroblast infiltration, collagen deposition and re- epithelialization were evaluated. Results revealed that the edema score significantly decreased (P<0.05) in both treatment groups compared to the control group, whereas the neo-vascularization, fibroblast infiltration, collagen deposition and re- epithelialization significantly increased (P<0.05) in both treatment groups compared to the control group. These results showed that topical administration of fenugreek seed hydroethanolic extract promote skin full-thickness excision wound healing process in diabetic animal model.
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Pumpkin (Cucurbita moschata) is one of the vegetables that has high nutritional value and bioactive compounds. The aim of this study was to compare the effect of hydroethanolic and aqueous solvents on the functional potential and to investigate the bioactive compounds o More
Pumpkin (Cucurbita moschata) is one of the vegetables that has high nutritional value and bioactive compounds. The aim of this study was to compare the effect of hydroethanolic and aqueous solvents on the functional potential and to investigate the bioactive compounds of Pumpkin extract. For functional evaluation of extracts, the amount of ascorbic acid, total phenol and flavonoids, antioxidant capacity were performed by DPPH and FRAP methods and also the chemical compounds in the extracts were identified by GC/MS. The results showed highest levels of ascorbic acid, total phenol and flavonoids in the hydroethanolic extract were 98.66±13.29 mg/100g, 1.663±0.004 mg GA/100g and 0.381±0.01 mg QU/100g, respectively. Hydroethanolic extract showed the highest ability to inhibit DPPH radicals compared to aqueous extract in all prepared concentrations; so that hydroethanolic extract with a concentration of 800 μg/ml with 61.866% inhibition had the highest inhibitory properties. Detected chemical compounds in hydroethanolic and aqueous extracts using GC/MS were identified 16 and 7 chemical compounds, respectively, when the highest compound content in hydroethanolic extract equal to 34.86767% related to D-erythro-pentose, 2-deoxy and the aqueous extract was related to 5,2-dichlorophenol (74,2053%). The results of this study suggest the use of hydroethanolic solvent for higher extraction of bioactive compounds from pumpkin.
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The healing of full-thickness wounds has always attracted attention due to disruption of the healing process that leads to prolonged recovery period. Therefore, the quest for finding clinically effective wound-healing agents is important in the medical mana More
The healing of full-thickness wounds has always attracted attention due to disruption of the healing process that leads to prolonged recovery period. Therefore, the quest for finding clinically effective wound-healing agents is important in the medical management of full-thickness wounds. The present study was conducted to investigate the effects of Malva sylvestris hydroethanolic leaf extract on wound healing process in 54 male rats. Two standardized circular surgical full-thickness excisional wounds with a diameter of 7mm were created in the dorsal skin of vats in all three groups. The rats in the two treatment groups were treated with ointments containing 1.5% and 3% of the extract mixed with yellow soft paraffin, whereas the rats in the control group were only treated with yellow soft paraffin. Treatments were applied immediately after wounding and also for 16 consecutive days post-wounding. Wound-healing was assessed microscopically in terms of the initiation of neo-dermal tissue deposition and wound closure over a 16-day period. Significant increase in wound contraction rate was seen in the treatment groups, especially the 3% group, compared to the control group (P<0.05). Moreover, the inflammatory cell score showed a decrease, whereas new vessel formation, fibroblast distribution, collagen production and epidermis thickness increased in Malva sylvestris hydroethanolic leaf extract-treated animals. These results suggest that the topical application of Malva sylvestris hydroethanolic leaf extract has beneficial effects on full-thickness excisional wound healing.
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