List of Articles Histopathological lesions

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  1 - Experimental study of histopathological lesions induced by ornithobacterium rhinotracheale infection followed inoculation with H9N2 avian influenza virus
  حسین گودرزی، آیدین عزیزپور، سعید چرخکار ، محمدحسن حبل‌الورید، رضا ممیز .
  Avian influenza virus as a primary agent causes histopathologic lesions in infected chickens. Although, it was not any complete histopathologic study of ORT effects on chickens infected with avian influenza virus. The purpose of this study was to evaluate histopathologi More

  Avian influenza virus as a primary agent causes histopathologic lesions in infected chickens. Although, it was not any complete histopathologic study of ORT effects on chickens infected with avian influenza virus. The purpose of this study was to evaluate histopathological lesions induced by secondary infection with Ornithobacterium rhinotracheale on infected specific pathogen-free (SPF) chickens with H9N2 AI virus. Sixty, one-day-old SPF chicks were provided and divided randomly into three groups. At 21 days, the chicks in the group 1 were inoculated with H9N2 AI virus, group 2 was first infected by AI virus and 3 days later inoculated by ORT. Each bird in control group was inoculated with PBS. Then, the birds were euthanized and examined for histopathological lesions. The samples from various tissues were collected at 2, 4, 6, 8, 10, 12, 14 and 16 days post-inoculation (DPI).  Histologic lesions in infected groups were observed as follow: congestion and hyperplasia of epithelium in trachea, pneumonia, hemorrhage and urinary tubules necrosis in kidneys, congestion of sinusoid spaces and portal vein in liver, lymphocyte depletion in the bursa of fabricius, hyperplasia of lymphoid Follicles in spleen, congestion and heterophil infiltration in thymus and increased of lymphoid Follicles in cecal tonsil. The results of this study showed that infected chickens by H9N2 AI virus under secondary infection with ORT cause increased histopathological lesions induced by virus. Manuscript profile
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  2 - An experimental study of histopathological lesions of secondary infection with H9N2 avian influenza virus following inoculation by Ornithobacterium rhinotracheale in SPF chickens
  آیدین Azizpour زهرا Amirajam راحم , khoshbakht نورالدین Hosseinpour Azad
  10.30495/jcp.2023.22494
  The purpose of this study was to evaluate the type, severity and extent of tissue lesions induced by secondary infection with avian influenza virus following inoculation by ORT in SPF chickens. Sixty, one-day-old SPF chicks were provided and divided randomly into three More

  The purpose of this study was to evaluate the type, severity and extent of tissue lesions induced by secondary infection with avian influenza virus following inoculation by ORT in SPF chickens. Sixty, one-day-old SPF chicks were provided and divided randomly into three groups. At the age of 21 days, the chicks in the first group were inoculated only with ORT and second group inoculated first with ORT and three days later with H9N2 virus. Each bird in third group was received only with PBS as control. Tissue samples were taken from trachea, lungs, liver, kidneys, bursa of fabricius, thymus, spleen and cecal tonsils for histopathological studies and after preparation and tissue sections were stained by Hematoxylin and Eosin (H&E) method. In chickens of the first group, ORT bacteria in addition to cause tissue lesions in organs of the respiratory system (loss of cilia with infiltration of mononuclear cells under the mucosa in the trachea, hyperemia and pneumonia in the lung), liver (congestion of sinusoids and central veins) and the kidneys (Hemorrhage and necrosis of urinary tubes) in  immune system organs also cause tissue lesions such as lymphocyte depletion in the bursa of fabricius, congestion and heterophil infiltration in thymus and increase in the of lymphoid follicles in the spleen and cecal tonsils. The histopathological lesions in chickens of the second group was more severe and longer than that of the first group. Tissue changes were not observed in control group. The results of this study showed that in chickens inoculated by ORT and under secondary infection with avian influenza virus cause exacerbate and prolongation histopathological lesions induced by ORT. Manuscript profile