List of Articles Heat shock

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  1 - Cloning of Mycobacterium avium paratuberculosis hsp70 gene into pET-24a plasmid and its expression in Escherichia coli
  Rasa Sheini mehrabzade Masoudreza Siefi abad shapori Masoud Ghorbanpoor Darush Gharibi
  10.30495/jvcp.2022.1954420.1362
  Johne's disease (Paratuberculosis) is a disease caused by Mycobacterium avium paratuberculosis (MAP) which is a chronic and progressive infection that chiefly affects ruminants. The disease can lead to significant economic losses in the livestock industry and may also b More

  Johne's disease (Paratuberculosis) is a disease caused by Mycobacterium avium paratuberculosis (MAP) which is a chronic and progressive infection that chiefly affects ruminants. The disease can lead to significant economic losses in the livestock industry and may also be a threat for public health; because it may be transmitted to humans through consumption of milk and other contaminated animal products. Immunity and resistance against the Johne's disease is mainly due to cell mediated immune response. Heat Shock Protein 70 (HSP70) of this bacterium is one of its important proteins, which the immune response against it can prevent the fecal excretion of bacteria. In order to facilitate the production of recombinant vaccine against Johne's disease, in this study the recombinant HSP (recombinant HSP; rHSP) was produced and its immunogenicity investigated in rabbits. For this purpose, the hsp70 gene was cloned into pET-24a plasmid and the resulting recombinant plasmid was transferred to E. coli strain BL21. The expression of the above protein was checked by SDS-PAGE and the accuracy of the nucleotide sequence was confirmed by sequencing. Immunization of rabbits by rHSP70 resulted in the production of high levels of antibodies. Based on the findings, it seems that the HSP70 specific antibody can be evaluated in the design of diagnostic methods of the disease and the produced recombinant protein can be assessed for the production of recombinant vaccines. Manuscript profile
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  2 - Study of the effect of pioglitazone on testicular function after scrotal heat induced shock in the rat
  سعید Mohammad Hoseini مهرداد Neshat Gharamaleki سیداسماعیل Safavi
     Testicular heat shock even for a single short period can induce destruction in testicular tissue and disturbances spermatogenesis. Pioglitazone is an anti-inflammatory and anti-oxidative drug which is used widely in diabetic patients. The main aim of this s More

     Testicular heat shock even for a single short period can induce destruction in testicular tissue and disturbances spermatogenesis. Pioglitazone is an anti-inflammatory and anti-oxidative drug which is used widely in diabetic patients. The main aim of this study is evaluation of effect of pioglitazone on testicular tissue after scrotal heat shock. For this purpose, 54 male Wistar rats were randomly divided in to 9 of 6 rats. In the first three groups which were used as control, the scrotum of animals were submerged in 230c water. In the other three groups (treatment 1), the scrotum was submerged in 430c water for 15 minutes and then the animals received normal saline for 15, 30 and 60 days. In the final three groups (treatment 2) after scrotal heat shock induction, pioglitazone was injected at a dose of 20 mg/kg for 15, 30 and 60 days. At the end of the experimental periods, testicular tissue samples were obtained for pathological and histomorphometerical studies. The results were analyzed with one-way ANOVA and Tukey test and p Manuscript profile
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  3 - بررسی بیان پنج ژن مرتبط با تنش در مرغان بومی خراسان تحت تنش گرمایی حاد
  ر. توحیدی م.ر. نصیری ع. جوادمنش آ. جوانمرد
  دمای بالا یکی از فاکتورهای اصلی محیطی مؤثر بر کاهش سود اقتصادی واحدهای پرورش طیور است زیرا، رشد و تولید پرنده را مختل می‎کند. پروتئین‎های شوک گرمایی (HSPs) نقش کلیدی در مکانیسم دفاعی سلولی علیه گرمای محیطی دارند. هدف از انجام مطالعه حاضر، بررسی میزان بیان ژن&lrm More

  دمای بالا یکی از فاکتورهای اصلی محیطی مؤثر بر کاهش سود اقتصادی واحدهای پرورش طیور است زیرا، رشد و تولید پرنده را مختل می‎کند. پروتئین‎های شوک گرمایی (HSPs) نقش کلیدی در مکانیسم دفاعی سلولی علیه گرمای محیطی دارند. هدف از انجام مطالعه حاضر، بررسی میزان بیان ژن‎های کاندیدا در کبد مرغان بومی خراسان تحت تنش گرمایی حاد بود. برای این منظور 16 جوجه 42 روزه به دو گروه تقسیم شدند؛ شاهد (دمای محیطی  °C25 و رطوبت 50 درصد) و تحت تنش گرمایی (دمای محیطی  °C42 و رطوبت 50 درصد)، و سپس از کبد آن‎ها نمونه‎برداری شد. سطح بیان ژن‎های HSPB1، HSPB9، SERPINH1، HSPA2 و HSP110 با روش RT-qPCR اندازه‎گیری شد. نتایج تجزیه واریانس نشان داد که بیان ژن‎های HSPA2 و HSP110 به طور معنی‎داری افزایش داشت. سه فرایند از فرایندهای بیولوژیکی هستی‎شناسی ژنی دارای FDR کمتر از 0.01 بودند. ژن‎های HSPA2 و HSPB1 در این فرایندها شرکت داشتند که باعث تحریک سلول‎ها در برابر افزایش دما می‎شدند. این نتایج نشان می‎دهند که مرغان بومی خراسان مقاومت مطلوبی در برابر تنش گرمایی حاد دارند. همچنین، ژن HSPA2توانایی بیان زیاد در دمای محیطی بالا برای حفظ ساختار پروتئین‎های سلولی را دارد. Manuscript profile
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  4 - رفتار روزنه ای برگ بیدی (Tradescantia pallida) در واکنش به دمای محیط ریشه
  نبی الله اشرفی عبدالحسین رضایی نژاد
  تاثیر دمای محیط ریشه (25، 35 و 45 درجه) و مدت تیمار گرمایی (30، 60 و 90 دقیقه) بر تبادلات گازی و رفتار روزنه­ای در برگ گیاه برگ بیدی بررسی گردید. آزمایش در محیط کنترل شده و به صورت  فاکتوریل بر پایه طرح کاملا تصادفی با چهار تکرار انجام گرفت. نتایج نشان داد میزا More

  تاثیر دمای محیط ریشه (25، 35 و 45 درجه) و مدت تیمار گرمایی (30، 60 و 90 دقیقه) بر تبادلات گازی و رفتار روزنه­ای در برگ گیاه برگ بیدی بررسی گردید. آزمایش در محیط کنترل شده و به صورت  فاکتوریل بر پایه طرح کاملا تصادفی با چهار تکرار انجام گرفت. نتایج نشان داد میزان فوسنتز در گیاهان تیمار شده با دمای 25 و 35 درجه اختلاف آماری معنی­داری نداشته است. اگرچه، مساحت منفذ روزنه و عرض منفذ روزنه در دمای 35 درجه در مقایسه با دمای 25 درجه افزایش نشان داد ولی در دمای 45 درجه به شدت کاهش داشته است. همچنین میزان فتوسنتز و هدایت روزنه­ای گیاهان تیمار شده با 45 درجه در مقایسه با دمای 35 درجه به ترتیب 76 و 68 درصد کاهش داشته است. مساحت منفذ روزنه در گیاهان تیمار شده با دمای 35 درجه در مقایسه با دمای 25 و 45 درجه به ترتیب 27 درصد و 320 درصد بیشتر بوده است. مساحت منفذ روزنه بعد از 30 دقیقه تیمار با دمای 35 درجه، به­ترتیب 61 و 45 درصد بیشتر از زمان 60 و 90 دقیقه تیمار بوده است. شوک گرمایی 45 درجه در محیط ریشه در مقایسه با دمای 35 درجه موجب کاهش هدایت روزنه ای (81 درصد) و تعرق  (60 درصد) می­گردد. به طور کلی، نتایج نشان داد که دمای ریشه بر رفتار روزنه­ای و تبادلات گازی موثر بوده و می­تواند در تولید گیاهان گلخانه­ای و در برنامه تغذیه مورد توجه قرار گیرد. Manuscript profile
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  5 - Genetic Evaluation and Population Structure of the Gene Locus Involved in Heat Stress (HSP90β) in Native Chickens of Mazandaran, West Azerbaijan and Commercial Chickens
  Jafar Pish Jang Aghajeri
  10.22034/ascij.2023.1982273.1477
  In hot and dry regions, heat stress causes significant economic losses in poultry production and leads to the reduction of several physiological and metabolic factors. This research was conducted in order to investigate the allelic and genotypic polymorphisms of the gen More

  In hot and dry regions, heat stress causes significant economic losses in poultry production and leads to the reduction of several physiological and metabolic factors. This research was conducted in order to investigate the allelic and genotypic polymorphisms of the gene locus involved in heat stress (HSP90β) in native chickens of Mazandaran, West Azerbaijan and commercial broiler and egg-laying chicken populations using PCR-RFLP technique. Randomly, blood was taken from 400 chicken and genomic DNA was extracted by salting out method. The desired gene locus of 494 base pairs was amplified with the help of specific primers and MspI enzyme digestion was used to identify the mutation in the gene locus. For this gene locus, two types of genotypes M1M1 and M1M2 and two alleles M1 (with one band of 494 bp) and M2 (with two bands of 248 bp and 246 bp) were identified. Mazandaran, West Azerbaijan native and commercial broiler chicken masses were in the Hardy-Weinberg equilibrium. For Mazandaran, West Azerbaijan native and commercial broiler chicken populations, Shannon information index was 0.32, 0.28 and 0.40, respectively, the fixation index was 0.12, -0.11 and -0.16, respectively and the observed heterozygosity was 0.19, 0.17 and 0.28, respectively. Due to the presence of polymorphism and mutation in this gene locus, it is possible to use this marker in the native chickens of Mazandaran and West Azerbaijan with genetic selection and in commercial broiler chickens with genetic selection at the line level, to remove heat-sensitive chickens and keep them heat-resistant chickens are used. Manuscript profile
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  6 - Alterations of Serum Adenosine deaminase, Total Sialic acid, Malondialdehyde and Heat Shock Protein-27 in Sheep with Naturally Infected Liver Cysticercus taeniaculis
  Kaveh Azimzadeh hooman azizizadeh
  The present study is involved investigation of total sialic acid (TSA), adenosine deaminase (ADA), malondialdehyde (MDA) and heat shock protein-27 (HSP-27) alterations in sheep during Cysticercus taeniaculis infection. For this purpose, 40 parasitized and 40 healthy she More

  The present study is involved investigation of total sialic acid (TSA), adenosine deaminase (ADA), malondialdehyde (MDA) and heat shock protein-27 (HSP-27) alterations in sheep during Cysticercus taeniaculis infection. For this purpose, 40 parasitized and 40 healthy sheep were selected based on observation of cystic form in liver and lack of blood parasite along with no cystic conformation in carcass respectively. Thereupon, ten milliliters of blood were collected via the jugular vein from all ones and transferred to EDTA-contained and non-EDTA tubes and after preparation of plasma and sera, all parameters were detected in groups. The results revealed a significant decrease (p Manuscript profile
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  7 - Physiological reactions and thermal protein dynamics in coneflower under drought stress
  Kosar Moradi Fariba khalili
  10.22034/aej.2018.545685
  Plant cells have evolved to understand the various signals in their surroundings and respond to them by modulating the expression of genes. Drought is a natural and recurrent climatic feature in most parts of the world and plays an important and limiting role in crop yi More

  Plant cells have evolved to understand the various signals in their surroundings and respond to them by modulating the expression of genes. Drought is a natural and recurrent climatic feature in most parts of the world and plays an important and limiting role in crop yields. In this study, to ensure the stress on the medicinal herb of coneflower (Echinacea purpurea), the proline, potassium, phosphorus and nitrogen content of the leaves were evaluated in a completely randomized design with three replications, each of which was repeated in three pots. Also, expression of heat shock proteins in leaf tissue under four levels of drought stress irrigation at 25%, 50%, 75% and 85% of crop capacity was evaluated. The results showed a significant increase in the amount of proline, potassium, phosphorus and nitrogen in leaf tissue. Also, examination of thermal shock protein expression using Real-Time PCR indicated that drought stress significantly increased expression of heat shock protein in all studied treatments, which also proved the changes caused by stress.  In general, the coneflower plant resists some degree of resistance using of some protective mechanisms, such as increasing proline and heat shock proteins content. Manuscript profile