List of Articles Glutathione-S-transferase

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  1 - تاثیر گیاه Crinum glaucum A. Chev بر فعالیت آنزیمهای آنتی اکسیدانی اندوژن در استرس اکسیداتیو القا شده در یک مدل حیوانی
  اولوفون میلایو اوگونرینولا اولواسی اوگونرینولا اولواتومیلولا اولاتونجی اولوسگون فاجانا موتیو کاظیم ژمیسولا سایبو حبیب بانکول آدمولا آدیوی باباجید المو
  10.30495/medherb.2023.709441
  Background & Aim: The mechanism of action of Crinum glaucumbulb (Cgb) A. Chev (Amaryllidaceae) is enigmatic. Therefore, this study aimed at investigating the possible modulating properties of an aqueous extract of Cgb on endogenous antioxidant enzymes during lipopol More

  Background & Aim: The mechanism of action of Crinum glaucumbulb (Cgb) A. Chev (Amaryllidaceae) is enigmatic. Therefore, this study aimed at investigating the possible modulating properties of an aqueous extract of Cgb on endogenous antioxidant enzymes during lipopolysaccharide (LPS)-induced oxidative stress in a rat model.Experimental: 25 male and 25 female rats were divided into five groups (n=5) each: control group; treatment group, rats were given an aqueous extract of Cgb for 7 days; induced-oxidative stress group, rats were injected with LPS for 4 hours; post-Cgb group, rats were injected with LPS for 4 hours and treated with an aqueous extract of Cgb; and pre-Cgb group, rats were given an aqueous extract of Cgb for 7 days, injected with LPS for 4 hours, and treated with an aqueous extract of Cgb for 7 days. The blood, brain, heart, lungs, liver, and kidneys were harvested for the biochemical analysis. The endogenous antioxidant enzymes (catalase, superoxide dismutase, and glutathione-S-transferase) were analysed spectrophotometrically.Results: The hallmark of LPS is its ability to decrease the activity of oxidative stress marker enzymes, as observed in this study. The pre- and post-administration of an aqueous extract of Cgb significantly (P≤0.05) reversed the damaging effect of LPS by increasing the activities of catalase and superoxide dismutase in the blood and organs of male and female rats, respectively, while plasma glutathione-S-transferase activity was inhibited.Recommended applications/industries: The aqueous extract of Cgb has modulating properties to reduce the action of LPS-induced oxidative stress on endogenous antioxidant enzymes in male and female rats. Manuscript profile
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  2 - The effect of some antimalarial drugs on Plasmodium berghei glutathione-S-transferase enzyme
  Alireza Sadeghi Tafreshi Sedigheh Sadeghi Maryam Hekmatipour Shaghayegh Shams Maryam Mohammadi Farideh Vahabi Mohammad Arjmand Zahra Zamani
  Glutathione-S-transferase (GST) are a group of enzymes found in all living organisms that contribute to detoxification of xenobiotic compounds via Phase II conjugation with glutathione and play an important role in drug resistance. The GST activity in chloroquine-resist More

  Glutathione-S-transferase (GST) are a group of enzymes found in all living organisms that contribute to detoxification of xenobiotic compounds via Phase II conjugation with glutathione and play an important role in drug resistance. The GST activity in chloroquine-resistant strains of rodent and human Plasmodium is meaningfully higher than sensitive strains. In the present study, the GST activity and level were measured in those mice infected with Plasmodium berghei during the treatment with antimalarial drugs. Mice infections were carried out by P. berghei and inhibition growth of the parasite was performed through three doses of eosin B and prevalent antimalarial drugs similarly artemisinin and sulfadoxine-pyrimethamine in 24 h based on Peter's test. Two hours after the last dose, the mice were bled, RBCs were isolated by cellulose column, and P. berghei was released after washing with saponin. The enzyme function was measured using glutathione and CDNB as substrate at 340 nm. In addition, the GST levels were assessed by Biotech kit. The GST specific activities and levels of P. berghei in infected mice treated with eosin B and sulfadoxine-pyrimethamine and their combination were decreased while such change was not seen in those ones treated with atemisinin and in combination with eosin B. Owing to the reduction property of the combination of eosin B on the GST which has a fundamental role in the resistance of the parasite to antimalarial drugs, it could be considered as a promising procedure for application in antimalarial combination drugs. Manuscript profile