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      • Open Access Article

        1 - Effects of Thymus Powder (Thymus Vulgaris L.) on Egg Quality and Some Ovarian Parameters in Laying Hens
        Ahmad Minaei Javid Morteza Mehri Mohsen Sharafi
        An experiment was conducted in order to investigate the effect of different levels of Thymus vulgaris powder (0, 1, 2, and 3%) on performance, egg quality, some ovarian parameters, and level of malondialdehyde in the liver and follicular fluid of Hy-Line w36 laying hens More
        An experiment was conducted in order to investigate the effect of different levels of Thymus vulgaris powder (0, 1, 2, and 3%) on performance, egg quality, some ovarian parameters, and level of malondialdehyde in the liver and follicular fluid of Hy-Line w36 laying hens. The hens were divided into 4 equal treatment groups and each treatment consisted of 4 replicates in a completely randomized design. In the period of 26 to 33 weeks of age, no significant difference was observed among the dietary treatments in terms of egg production, shell-less egg ratio, feed intake, and feed conversion ratio; but egg weight and egg mass were affected by 2% thymus powder  (P<0.05). In the period of 34 to 39 weeks of age, shell-less eggs (%), egg production (%), and feed efficiency were not affected by thymus powder, but feed intake, egg mass, and egg weight were significantly affected by treatment 3 (P<0.05). The egg quality characteristics were measured at the end of weeks 26, 30, 34, and 38 of age. The results showed that only in week 26, the Haugh unit was significantly increased by 1 and 2% thymus powder (P<0.05). At the end of the experiment, the number of white, large yellow, and small yellow follicles, and level of malondialdehyde in the liver and follicular fluid were recorded and none of these were not affected by different levels of thymus powder. Overall, the experimental results showed that the use of 2% thyme powder in the laying hen diet improved the egg mass and egg weight, and also increased the Haugh unit during the peak production period.  Manuscript profile
      • Open Access Article

        2 - Effect of Goat Follicular Fluid on in vitro Production of Embryos in Black Bengal Goats
        S.A. Masudul Hoque M.A.M. Yahia Khandoker S.K. Kabiraj L.Y. Asad M. Fakruzzaman K.M.A. Tareq
      • Open Access Article

        3 - The Comparison of 24 and 48 Hours Culture on In vitro Maturation of Oocyte using Menstrual Blood Stem Cells Secretome in Polycystic Ovary
        Hilda Rastegari نسیم حیاتی رودباری Somaieh Kazemnejad Soheila Ansaripour
        The application of mesenchymal stem cells is a novel approach in regenerative medicine and infertility. Recently, culture enrichment using the secretome obtained from these cells, as well as the optimal time for culture, have been considered in order to improve the resu More
        The application of mesenchymal stem cells is a novel approach in regenerative medicine and infertility. Recently, culture enrichment using the secretome obtained from these cells, as well as the optimal time for culture, have been considered in order to improve the results of in vitro maturation (IVM), especially in women with polycystic ovaries (PCOS). The purpose of this study is comparing 24 and 48 timing on IVM using secretome of menstrual blood stem cells (MenSCs) along with follicular fluid and melatonin in PCOS women. 400 germinal vesicle oocytes were collected from 100 PCOS patients, as the best candidates for IVM, and randomly divided into four groups: control, secretome, follicular fluid and melatonin. Oocyte maturation was evaluated at 24 and 48 hours. Also, the effect patient’s age on the results of the study was evaluated in the age groups under 30 and over 30 years old in both time periods. Oocyte maturation rate showed a significant increase in 24 hours in the group enriched with secretome compared to the control (p < 0.05). Also, matured oocytes were noticeably higher in melatonin enriched group, in 48 hours, compared to the control (p < 0.001). Moreover, according to our findings, the age of the women did not have a significant effect on the oocyte maturation rate in the 24-hour culture, while in the younger age group, the oocyte maturation rate increased significantly both in secretome and melatonin groups compared to the control group. As a result, the culture time of 24 hours with IVM medium enriched by secretome is the optimal time in order to increase oocyte maturation in PCOS women. Also, the use of melatonin seems to be an effective strategy to improve egg maturation in extended culture times. Manuscript profile
      • Open Access Article

        4 - Studying the Concentration of Glucose, Urea and Cholesterol in Blood Serum and Follicular Fluid of Different-Sized Follicles in Hybrid Cows of Khouzestan Province
        کمال Hasanpoor, محمد تقی Tagi Begi Nasiri, منصور Khezri, , M. Asghari Moghadam A. Karamzade, M. Amrai,
        Improvement of the fertility and reproductive status of cows necessitates having suffcient knowledge of the ovary of this animal species. Ovarian follicular activities play a signifcant role in controlling reproductive cycle, ovulation and pregnancy. The present study More
        Improvement of the fertility and reproductive status of cows necessitates having suffcient knowledge of the ovary of this animal species. Ovarian follicular activities play a signifcant role in controlling reproductive cycle, ovulation and pregnancy. The present study aims to compare the concentration of glucose, urea and cholesterol of ovarian follicular fluid in different-sized follicles with the blood serum of hybrid cows. Blood samples and ovaries of 20 slaughtered cows were examined. The follicular fluid was obtained from 3 follicular groups of small (3-5mm), average (6-9mm) and large (10mm or more) sizes. Concentration of glucose, urea and cholesterol in follicular fluids and blood serum was determined. The results showed that concentration of glucose, urea and cholesterol in blood serum was signifcantly higher than that of the large, average-sized and small follicles. Glucose concentration of large follicles was signifcantly higher than that of the average-sized and small follicles (P &lt; 0.05). However, no signifcant difference was observed between the average-sized and small follicles. Urea level decreased as the follicle size increased. Cholesterol concentration of small follicles was signifcantly higher than that of the large and average-sized follicles (P &lt; 0.05). However, no signifcant difference was observed between the large and average-sized follicles. Overall, the results showed that ovarian follicle size grows and matures in a changing biochemical environment. This environment is associated with the changes in blood glucose level. Moreover, urea and cholesterol levels decrease as the follicle size increases. &nbsp; Manuscript profile