List of Articles Flow Cytometry

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  1 - Quantitative and qualitative assessment of cells in several commercial probiotic products and evaluation of storage conditions on cell culturability and viability of a probiotic yeast
  Fatemeh Hakimipour Rasoul Shafiei Mohammad Rabbani khorasgani
  One of the essential criteria for determining the quality of products containing probiotics is the presence of a certain number of viable cells per gram of product. To transmit enough viable cells to the gastrointestinal tract, the population of probiotic microorganisms More

  One of the essential criteria for determining the quality of products containing probiotics is the presence of a certain number of viable cells per gram of product. To transmit enough viable cells to the gastrointestinal tract, the population of probiotic microorganisms should be preserved during storage. In the present study, culturable cells of some commercial products were isolated and enumerated by the plate count and flow cytometric methods. The isolates were then identified by biochemical and molecular methods. The viability of yeast cells was then assessed at different temperatures during 30 days by the methods above. The results showed that Protexin restore® contained the same types and number of bacteria that were claimed by the manufacturer. In contrast, protexin balance® and Dailyeast® contained a lower number of bacterial cells and yeast cells, respectively. It is noteworthy that only 33% of yeast cells were culturable on YPG agar, while flow cytometric analysis showed that 87% of cells were viable and exhibited cell membrane integrity. Also, yeast cells in Dailyeast® product were stable at 4-35°C. In conclusion, using different culture media and physicochemical conditions for the enumeration of viable and culturable cells may lead to different results. Thus, a combination of different methods should be used to get good results. Manuscript profile
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  2 - Influence of Arsenic (III), Cadmium (II), Chromium (VI), Mercury (II), and Lead (II) Ions on Human Triple Negative Breast Cancer (HCC1806) Cell Cytotoxicity and Cell Viability
  Tsdale F. Mehari Checo J. Rorie Sherette S. Godfrey Radiah C. Minor Sayo O. Fakayode
  10.22034/jchr.2017.544160
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  3 - The Apoptotic Effects of Saccharomyces cerevisiae Cytoplasmic Extract on MCF-7 Cell Line
  Ahmadreza Gholamian Mohammad Heidarpour Hadi MohebAlian
  10.22034/ascij.2023.1983579.1481
  Although many studies have been conducted on the anticancer effects of plant extracts, few studies have been done on the anticancer effects of fungal extracts. The aim of this study was to investigate the apoptotic effects of Saccharomyces cerevisiae cytoplasmic extract More

  Although many studies have been conducted on the anticancer effects of plant extracts, few studies have been done on the anticancer effects of fungal extracts. The aim of this study was to investigate the apoptotic effects of Saccharomyces cerevisiae cytoplasmic extract on MCF-7 cell line. In this experimental laboratory study, MCF-7 breast cancer cells were treated with 500, 1000, and 2000 µg/mL of Saccharomyces cerevisiae extract for 24, 48, and 72 hours. Cell viability was assessed using MTT assay and cellular apoptosis was determine using flow cytometry. The data were analyzed using one-way analysis of variance. The results of the MTT test showed the percentage of cell viability decreased corresponding to an increase in the concentration and the treatment duration of the extract such that the lowest percentage of cell viability was observed at a concentration of 2000 and a duration of 72 hours. The flow cytometry results confirmed apoptotic cell death in cancer cells. The results of this study indicate the cytotoxic effects of Saccharomyces cerevisiae extract on breast cancer cells. The flow cytometry results confirmed that the cytotoxic effects were due to the induction of apoptosis in cancer cells. Manuscript profile