List of Articles ESBLs

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  1 - Detection of bla TEM, bla OXA and bla SHV genes in Escherichia coli isolated from colibacillosis in poultry by multiplex-PCR
  zohreh mehranifar mitra salehi kiyoumars amini
  Abstract    Antimicrobial resistance exhibited by extended-spectrum b-lactamases (ESBLs) production by E. coli is considered to be a major threat for colibacillosis in poultry. The present study deals with the detection of bla TEM, bla OXA and bla SHV genes i More

  Abstract    Antimicrobial resistance exhibited by extended-spectrum b-lactamases (ESBLs) production by E. coli is considered to be a major threat for colibacillosis in poultry. The present study deals with the detection of bla TEM, bla OXA and bla SHV genes in E. coli isolated from colibacillosis by MPCR.A total of 60 strains of E. coli were recovered from the poultry in Kerman, Iran. Cellular DNA was extracted by CinnaPure-DNA (Cell culture, Tissues, Gram negative Bacteria and CSF) and MPCR was performed for the identification of the OXA, SHV and TEM genes.CDT test results showed that 45 strains (75%) were noted for ESBL production. A total of 19 and 13 strains (31.6% and 21.6%) were positive for blaOXA and bla aada, respectively and 7 isolates (11.6%) carried both OXA and aada genes. All isolates were negative for TEM and SHV genes.The results showed, although disk diffusion is a highly practical and cost effective method for identification of ESBLs, but the novel multiplex PCR assay designed in this study may be routinely used in veterinary diagnostic laboratory for identification of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae. Manuscript profile
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  2 - Frequence of ESBLs and Antibiotic Resistant Pattern in to E.coli and K.pneumoniae Strains Isolated of Hospitalized and Out patients Acquired Urinary Tract Infection (Esfahan/2008-2009)
  Shilla Jalalpoor Sina Mobasherizadeh
  Introduction and Objectives: Escherichia coli and Klebsiella pneumoniae are the most agent of urinary tract infection (UTI) and prevalence Extended-Spectrum Beta-lactamase (ESBLs) in these bacteria due to spread of antibiotic resistance and mortality and morbidity in pa More

  Introduction and Objectives: Escherichia coli and Klebsiella pneumoniae are the most agent of urinary tract infection (UTI) and prevalence Extended-Spectrum Beta-lactamase (ESBLs) in these bacteria due to spread of antibiotic resistance and mortality and morbidity in patients. The best manner for control of ESBLs in bacteria, are inhibition of spread these bacteria and use of standard method for recognizes ESBLs producer strains. Subject of this study was comparison frequency of ESBLs in Escherichia coli and Klebsiella pneumonia strain in UTI acquired patients with phenotypic test. Materials and Methods: This cross-sectional search was performed in Azzahra and Shariaty hospitals during of 2008-2009 years in Esfahan, according to statistical formula randomly selected 91 samples from urinary infections. Bacterial identification was performed with microbiological methods, ESBLs production was performed with screening and confirmatory test and survey antibiotics resistant pattern was performed with Kirby method. Results: Frequence of ESBLs in E.coli and K.pneumoniae strains was respectively 47.97% and 41.66% .According to antibiogram result respectively 59.2%, 54.9%, 30.3%, 27.8%, 19.5% and 16.7% of E.coli strains were resistant into Co-Trimoxazole, Nalidixic acid, Ciprofloxacin, Gentamicin, Ceftazidime and Nitrofurantoin and respectively 75%, 50%, 40%, 44.5%,37.5%, 37.5%, 22.3% and 0% of K.pneumoniae strains were resistant into Ampicillin, Co-Trimoxazole, Nitrofurantoin, Ceftazidime, Amikacin, Cephotaxime, Imipenem and Ciprofloxacin. Conclusion: The result showed high frequence of ESBLs, so  antibiotic resistant in isolated bacteria from hospitalized into out patience's that represent high spread  antibiotic resistant strains in hospitals. Manuscript profile