List of Articles Dot ELISA

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  1 - Designing Dot ELISA kit for detection of Staphylococcus aureus SEA enterotoxin and Sandwich ELISA compare, its validation in pastry cream bread
  , SA. Bathaei , H. Ahari , AA Anvar
  In this study, detection and recognition of Staphylococcus aureus enterotoxin was performed by designing a Dot ELISA sensitive kit. In the present study, nitrocellulose membrane (NCM) with 0.22 micron pores was applied as the bed, and 1:500 diluted specific antibody of More

  In this study, detection and recognition of Staphylococcus aureus enterotoxin was performed by designing a Dot ELISA sensitive kit. In the present study, nitrocellulose membrane (NCM) with 0.22 micron pores was applied as the bed, and 1:500 diluted specific antibody of Staphylococcus aureus enterotoxin was coated on the NCM. Next, The HRP-tagged antibody was added with dilution of 1/2000, and the substrate was added gradually and rewashed. In this experiment, the samples were manually contaminated and then compared with the ELISA sandwich method as a gold standard. The kit is capable of detecting 50 ng/ml and higher of toxin A, and if the amount of toxin is less, it will report it negatively. In this test, of which 40 samples were collected and 4 positive cases were reported, it was revealed that about 10% of the collected samples were contaminated. Nowadays, using the Dot ELISA kit as a quick method with relative sensitivity and properties, it is possible to improve the repeatability of detecting Staphylococcus enterotoxin A in creamy candies. Manuscript profile