List of Articles CML

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  1 - An Effective Modality Conflict Model for Identifying Applicable Policies During Policy Evaluation
  Hamidah Ibrahim Fatimah Sidi Nur Izura Udzir Poh Kuang Teo
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  2 - Quantitative Assessment of CK-18 Gene Expression in Patients Suffering from Chronic Myeloid Leukemia
  Farshid Ghaidar Nastaran Asghari Moghaddam fariba Sharifnia
  Chronic Myeloid Leukemia (CML) is one of the most common cancers. Its incidence has been increased during recent years and leads to high mortality if it is diagnosed late. Currently, most laboratory and diagnostic methods are difficult, cost- and time- consuming and thi More

  Chronic Myeloid Leukemia (CML) is one of the most common cancers. Its incidence has been increased during recent years and leads to high mortality if it is diagnosed late. Currently, most laboratory and diagnostic methods are difficult, cost- and time- consuming and this is the major obstacle to early detection of cancer. Cytokeratin 18 (CK18) is one of the cytoskeletal proteins which expression pattern change has been observed in various cancers. The present study investigates the altered expression of this gene at mRNA level in people who with recently diagnosed CML. In order to achieve this goal, blood samples were collected from 50 CML patients and 50 healthy individuals. Then, RNA extraction was performed and relative expression of CK-18 gene was measured by Real-time PCR. Data were analyzed by 2-ΔΔCT. Biomarker potential was evaluated by ROC curve. Results showed a 1.92-fold increase in the expression of CK-18 gene in leukemia samples compared to normal ones (p<0.05). No significant relationship was found between gender and changes in gene expression in case group. The results of this study elaborate that evaluation of CK-18 mRNA could be useful to detect CML along with other factors. Manuscript profile
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  3 - Phytochemical examination of the hydroalcoholic extract of Polylophium involucratum (Pall.) Boiss. harvested from the heights of the Javaherdeh - Ramsar and determination of its cytotoxic effects on chronic myeloid leukemia
  Shahab Ojani Naser Montazeri Masoud Mohammadi Zeydi Masoud Ghane
  10.30495/zisti.2023.1993274.1169
  Introduction: Nowadays, the emergence of allergies, side effects of drugs, antibiotic-resistant strains, and the need of body to antioxidants emphasized the importance of herbal medicines.Aim: Therefore, this project aims to investigate the phytochemical extract of hydr More

  Introduction: Nowadays, the emergence of allergies, side effects of drugs, antibiotic-resistant strains, and the need of body to antioxidants emphasized the importance of herbal medicines.Aim: Therefore, this project aims to investigate the phytochemical extract of hydroalcoholic of Polylophium involucratum harvested from the heights of the Javaherdeh - Ramsar and determine its cytotoxic effects on chronic myeloid leukemia.Material and methods: To this end, Polylophium involucratum seeds were collected from the heights of the Javaherdeh, Ramsar, and extracted using a microwave assisted extraction. Then, using quantitative and qualitative phytochemical tests, determining antibacterial activity by disk emission method and finally, assessing the effect of cytotoxicity on the category of K562 cancer cells using the MTT method was investigated.Results: Phytochemical screening of the hydroalcoholic extract of Polylophium involucratum confirmed the presence of secondary metabolites such as flavonoids, terpenoids, coumarins, cardiac glycosides, tannins, phenols, quinones, and saponins. The total amount of phenolic compounds and flavonoid compounds was calculated as 12.93±2 and 7.58±7 mg/ml respectively. The percentage of free radical inhibition was obtained at 57.70±0.5 and the IC50 value was 0.66 μg/ml. The aura diameter of non-growth was observed in Gram-negative Escherichia coli bacteria and Gram-positive Staphylococcus aureus and Bacillus cereus 25, 10, and 10 mm respectively. The results of cellular toxicity also showed that Polylophium involucratum hydroalcoholic extract is dose-dependent, with the highest toxicity effect at a concentration of 50 μg/ml, and the highest bioavailability effect at a concentration of 20 μg/ml in 24 hours, with an IC50 level equal to 50.35±0.03 μg/ml with a meaningful level of 5%.Conclusion: Based on the obtained results, in general, the use of effective plant compounds is considered one of the most effective strategies in the treatment of cancer, and the secondary metabolites in Polylophium involucratum extract can be a promising method in the treatment of cancer, which requires more studies and experiments are to be done in the future. Manuscript profile
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  4 - Cytotoxic Effect of Seed Essential Oil of Zizyphus jujuba on K562 Leukemia Cell Line
  Elham Ghodousi Dehnavi H. Maghsoudi R. Haji Hosseini
  Essential oils are complex mixtures of several components endowed with a wide range of biological activities, including anticancer properties. Due to the side effects and the resistance of synthetic drugs, plant products are considered for the treatment of cancer. This More

  Essential oils are complex mixtures of several components endowed with a wide range of biological activities, including anticancer properties. Due to the side effects and the resistance of synthetic drugs, plant products are considered for the treatment of cancer. This study is performed the effect of cytotoxicity of essential oil from the seeds of Zizyphus jujuba (EOSZ) on K562 cell line as a model of chronic myeloid leukemia. In this study, the seeds of Z. jujube were collected from the Isfahan city, and essential oils were extracted by Clevenger apparatus and then the combination by gas chromatography and spectrophotometry were identified. K562 cells were cultured and treated with concentrations of essential oils of 1 to 900 micrograms per milliliter (in 24h, 48h, and 72h). Cytotoxicity of essential oil from the seeds of Zizyphus jujuba (EOSZ) against K562 leukemia cells was estimated by the MTT assay. The absorbance was measured using ELISA plate reader (540 nm) were measured. Then the data were analyzed with the computer software SPSS version 15.0 (SPSS Inc., Chicago, USA), t.test and ANOVA. 24 compounds were identified in the essential oil, the main component of which was Eugenol. Essential oil from the seeds of Zizyphus jujuba (EOSZ), the highest cytotoxic effect in LC50 = 200 micrograms per milliliter and 72 hours after treatment. In other words, the seed essential oil from Zizyphus jujuba (EOSZ) expresses the effects of cellular toxicity on k562 cells, depending on the dose and time. Considering the toxicity of seed essential oil from Zizyphus jujuba (EOSZ), seed essential oil from Zizyphus jujubacan be considered a Potential candidate for further CML studies, and other cancer cells. Manuscript profile