List of Articles Antibiotic susceptibility

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Prevalence and antibiotic susceptibility pattern of Yersinia enterocolitica in broiler meat and edible offal at Ardabil retails
  S. Shekari M. Ghiami Rad
  Yersinia enterocolitica is one of the most important food-borne bacteria and it can cause yersiniosis. This study was conducted to investigate the occurrence of Y. enterocolitica in poultry meat and offal marketed in Ardabil, as well as to assess the antibiotic resistan More

  Yersinia enterocolitica is one of the most important food-borne bacteria and it can cause yersiniosis. This study was conducted to investigate the occurrence of Y. enterocolitica in poultry meat and offal marketed in Ardabil, as well as to assess the antibiotic resistance pattern of the isolates. In this descriptive cross-sectional study, a total of 344 samples including 264 poultry meat and 80 offal samples were randomly obtained during the spring and summer of 2016. The samples were investigated for the presence of Y. enterocolitica using Iranian National Standard. Moreover, antibiotic susceptibility was examined by Kerby-Bauer method. Based on results, 22.34% of the poultry meat samples and 37.5% of the offals were contaminated with Y. enterocolitica. Antibiogram results revealed that 84.74% of the isolates were resistant to Ampicillin; in the cases of Cephalothin and Cephalexin, it was estimated at 69.49% and 64.4% of the isolates, respectively. However, resistance was observed for Chloramphenicol and Syprufluxasine. Regarding the high occurrence Y. enterocolitica in chicken meat and offal and the presence of antibiotic-resistant strains of this bacterium, the implementation of hygienic measures to reduce the occurrence of Y. enterocolitica in chicken meat and the prudent use of antibiotics in the poultry industry to prevent the spread of strains resistant to antibiotics and transferring them to the human food chain is essential. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Prevalence and antibiotic susceptibility of Staphylococcus aureus in raw milks of Saqez
  M.T Farajpour M.H Sadeghi zali مهدی Ghiamirad
  Staphylococcus aureus is considered as a major foodborne pathogen worldwide which can contaminate milk either through mastitis or unsuitable processing conditions. The aim of this study was to determine the prevalence of S. aureus in raw milk of Saqez as well as to asse More

  Staphylococcus aureus is considered as a major foodborne pathogen worldwide which can contaminate milk either through mastitis or unsuitable processing conditions. The aim of this study was to determine the prevalence of S. aureus in raw milk of Saqez as well as to assess the antibiotic susceptibility profiles of the isolates. For this reason, 100 raw milk samples were obtained across Saqez. Presence of S. aureus was determined using conventional culture method; moreover, the antibiotic resistance pattern of the isolates was studied by disc diffusion method. Results indicated that 51 samples were contaminated by S. aureus. Amongst, 19 (37.25%) were resistant to at least 1 antibiotic, 19 (37.25%) to 2 antibiotics, 3 (5.88%) to 3 antibiotics, 5 (9.80%) to 4 antibiotics, 3 (5.88%) to 5 antibiotics, 1 (1.98%) to 6 antibiotic and 1 (1.98%) isolate was found resistant to 8 antibiotics. Results revealed a high prevalence rate of S. aureus in raw milks and also the board antibiotic resistance of the isolates Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Prevalence and antibiotic resistance pattern of Acinetobacter spp. infections in Shahrekord medical centers
  Tohid Piri Gharaghie Abbas Doosti Seyed Abbas Mirzaei
  10.30495/jdb.2021.686587
  the study estimated the prevalence and pattern of antimicrobial resistance of Acinetobacter spp. isolate from different clinical specimens. Primer design for Acinetobacter spp. was performed with Bioinformatics software Primer Express and Gene Runner. Primer authenticat More

  the study estimated the prevalence and pattern of antimicrobial resistance of Acinetobacter spp. isolate from different clinical specimens. Primer design for Acinetobacter spp. was performed with Bioinformatics software Primer Express and Gene Runner. Primer authentication was performed by BLASTn online tool and Sequence Match program. Acinetobacter isolates were identified from different clinical specimens by standard biochemical methods and PCR tests. Antimicrobial susceptibility tests and biofilm detection were performed for isolates identified by standard disk diffusion method according to CLSI‌ protocol and Microtiter plate. Acinetobacter spp. were identified by designed primer with Query Cover and Per. Ident 100%. From 60 clinical samples, 243 bacterial isolates were obtained. 131 isolates (53.9%) were related to gram-positive bacteria and 112 isolates (46.09%) were gram-negative bacteria, of which 43 isolates (17.69%) Were identified as Acinetobacter spp. According to the PCR test, 31 strains (77.5%) were identified as Acinetobacter baumannii, 7 strains (17.5%) as Acinetobacter lwoffii, 2 strains (5%) as Acinetobacter junii. Antibiotic susceptibility study showed that all isolated strains were MDR and 87.5% of isolates were XDR. However, only 12.5% of the isolates were sensitive to carbapenems, All Acinetobacter isolates were biofilm positive and were identified as strong biofilms with a total mean of 0.213. According to the study, it is clear that infection with Acinetobacter can lead to significant challenges in the country's health care system in the future. To this end, finding solutions to prevent infection of this bacterial genus, especially Acinetobacter baumannii, is very important and necessary. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  4 - Determination of antibiotic susceptibility pattern of Pseudomonas aeruginosa and the prevalence of bla OXA-23 gene in isolates from a Hospital in North Khorasan
  Mohadeseh Radmehr majid moghbeli
  10.30495/jmw.2021.690460
  Background & Objectives: Pseudomonas aeruginosa is one of the most important pathogens of opportunistic infections, which is considered as one of the main causes of nosocomial infections. The aim of this study was to isolate pseudomonas aeruginosa and identify strai More

  Background & Objectives: Pseudomonas aeruginosa is one of the most important pathogens of opportunistic infections, which is considered as one of the main causes of nosocomial infections. The aim of this study was to isolate pseudomonas aeruginosa and identify strains with                  bla OXA-23 gene using polymerase chain reaction (PCR) technique. Materials & Methods: In this study, 70 isolates of pseudomonas aeruginosa from various            infections including lung, urine, blood, wound and sputum were collected in north khorasan       hospital, after phenotypic and genotypic confirmation of the isolates, antibiotic discs of doripenem and imipenem (Rosco), meropenem and ciprofloxacin, colistin and amikacin (Mast) were used to perform antibiotic susceptibility testing of bacteria by disk diffusion method. Results: Isolates showed 32.8%, resistance to amikacin , 47.1% todoripenem, 37.1% to imipenem, 40% to ciprofloxacin, and 10% to colistin. The highest resistance of the strains was related to meropenem 61.4% and the lowest was colistin with 10% resistance. Conclusion: In this study, the resistance of pseudomonas aeruginosa to the antibiotic meropenem was 61.4% and compared to other studies, including in ethiopia, showed a higher level of           antibiotic resistance, this level of carbapenem resistance indicates an increase in resistance of pseudomonas aeruginosa strains to carbapenems.   Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  5 - Isolation and antibiotic sensitivity of E. coli K99 isolates from diarrheic calves at Fars province
  Zahra Shams Yahya Tahamtan Mohammad Kargar Seyed Mohammad Hosein Hoseini Seyed Ali Pourbakhsh
  Background and Objective: Diarrhea in newborn calves caused by enterotoxigenic Escherichia coli K99 is one of the important problems is the animal industrial husbandry. In this study we try to find the frequency of E. coli k99 in diarrheal newborn calf, and also determi More

  Background and Objective: Diarrhea in newborn calves caused by enterotoxigenic Escherichia coli K99 is one of the important problems is the animal industrial husbandry. In this study we try to find the frequency of E. coli k99 in diarrheal newborn calf, and also determine antibiotic susceptibility of isolates. Material and Methods: 312 rectal swabs of diarrheal calves were obtained in various parts of Fars province. The samples were investigated using biochemical test. All E. coli isolates tested for antibiotic susceptibility using disc diffusion method. Results: of 312 samples, 298 E. coli isolates were obtained, from which the slide agglutination method 13 isolates were identified Escherichia coli k99. These isolates were susceptible to enrofloxacin, nalidixic acid and flomocuen antibiotics. Also, there is some wide range of antibiotics resistance to in Escherichia coli k99. Conclusion: The prevalence of Escherichia coli k99 with diarrhea in calves was 4 / 3% in Fars province which compared to other reports ranking in the lower range. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  6 - The Frequency and Antibiotic Resistance Pattern of Aeromonas hydrophila and Yersinia ruckeri Isolated from Diseased Rainbow Trout Cultured in Chaharmahal and Bakhtiari Province
  Nima Panahi Far Mohsen Pournia Reza Salighehzadeh Marjan Mosafer
  10.22034/ascij.2023.1975527.1450
  Recognition of resistance and susceptibility of problematic bacteria such as Yersinia ruckeri and Aeromonas hydrophila in rainbow trout farms, in proper selection of antibiotics and infection control in breeding ponds. It plays an effective role. In this study, 100 dise More

  Recognition of resistance and susceptibility of problematic bacteria such as Yersinia ruckeri and Aeromonas hydrophila in rainbow trout farms, in proper selection of antibiotics and infection control in breeding ponds. It plays an effective role. In this study, 100 diseased fish were collected from rainbow trout farms in Chaharmahal and Bakhtiari province and after performing biochemical tests and purification of Aeromonas hydrophila and Yersinia ruckeri species, using PCR test with specific primers were finally approved. Antibiotic susceptibility of bacteria was investigated by disk diffusion method by culture on Müller-Hinton agar medium. Examination of the results of antibiogram of Aeromonas hydrophila isolates showed that the highest resistance was related to the antibiotics ampicillin (100%), colistin (80%), enrofloxacin (80%), flumquin (80%) and then, Compared to tetracycline (60%) and fluorophenicol (60%) antibiotics. The most sensitive isolates were related to trimethoprim-sulfamethoxazole (60%) and phosphomycin (60%) antibiotics. Examination of the antibiogram results of Yersinia ruckeri isolates showed that the highest resistance among the isolates was related to the antibiotics ampicillin (100%), erythromycin (100), trimethoprim-sulfamethoxazole (100%) and colistin (83.34%) and then showed about 67% resistance to tetracycline, fluorophenicol and phosphomycin antibiotics. Resistance to the antibiotics flumequin, lincomycin + spectinomycin (lincospectin) and enrofloxacin were also observed to be 50%, which means that none of these antibiotics would be a viable option for the treatment of bacterial infections caused by Yersinia ruckeri. In order to avoid growing bacterial resistance and to take the most effective decision in terms of antibiotic therapy, usage of antibiotics without antibiogram must be avoided. . Manuscript profile