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      • Open Access Article

        1 - Investigation of antibacterial effects of ethanolic extract of Sumac (Rhus coriaria L.) against Escherichia coli in vitro
        حمدالله Moshtaghi مریم Abbasvali الناز Mohammadi A.R Safian میلاد Adel
           The antibacterial effect of etbhanolic extract of Sumac (Rhus coriaria L.) was investigated quantitatively and qualitatively on Escherichia coli. The results of well diffusion test showed that extracts of Sumac in concentration of 0.5%, 1%, 2.5% and 5% coul More
           The antibacterial effect of etbhanolic extract of Sumac (Rhus coriaria L.) was investigated quantitatively and qualitatively on Escherichia coli. The results of well diffusion test showed that extracts of Sumac in concentration of 0.5%, 1%, 2.5% and 5% could inhibited E. coli. In this study it was shown that MIC of the alcoholic extract of Sumac against E. coli was 6.25 mg/ml and its MBC against this bacterium was 50 mg/ml. The results from evaluation of the antibacterial effects of the Sumac revealed that at 4 and 15 °C, the growth of E. coli in test tubes containing meat extracts has increased Throughout the 48 h of incubation period. Results showed that the growth of this bacteria in different concentration of Sumac extract as decreased in the both tested temperatures in comparison to time zero (p<0.05). Furthermore, there was a significant difference in the number of microorganism at various times between control and experimental groups in both tested temperatures (p<0.05).  Manuscript profile
      • Open Access Article

        2 - Antioxidant and antibacterial effects of laurus nobilis aqueous extract again Staphylococcus aureus and Escherichia coli
        B. Azimzadeh M. Jahadi M. Fazel
        Some medical plants which are rich in phenolic compounds (flavnoieds, tannis and anthocyanin) have drawn increasing attention as the most important naturanal antioxidant source by many developed countries. Laurus nobilis is one of the medical plants that grows in variou More
        Some medical plants which are rich in phenolic compounds (flavnoieds, tannis and anthocyanin) have drawn increasing attention as the most important naturanal antioxidant source by many developed countries. Laurus nobilis is one of the medical plants that grows in various regions of Iran. This plants is known to have many benefits and medical properties such as diuretics and mosaics. Also, this plant is used in treatment of gastrointestinal problems, especialy and it is effectieve in elimination of stomach gas. In this experiment, we studied antioxidant and antibacterial effects of Laurus nobilis plant. To this purpose, the efficiency of aqueous solvent extract, phenolic compounds, DPPH radical scavenging and ferric-reducing power and ABTS free radical scavenging were examined. Antibacterial characteristics of aqueous extract was evaluated on Staphylococcus aureus and Escherichiacoli. The result shows that the aqueous extract of Lauros nobilis has 14/8% extraction efficiency with high rate of phenolic (99/9±9/95) and the lowest IC50 in DPPH test (2/813) and the high rate of ferric reducing power (22/15±2/10) and ABTS free radical scavenging (22/87±2/03). The result of antibacterial test also indicates that aqueous extract has high antibacterial effect on Staphylococcus aureus (18±0) and E. Coli (18±0) mm.These facts show high antioxidant and antibacterial activity of laurel’s extract.  Manuscript profile
      • Open Access Article

        3 - Antimicrobial effects of Robinia pseudoacacia L. and Phytolacca americana L. ethanolic extracts against human bacterial pathogens in the habitat of northern Iran
        Nazila Rezanezhad Roghayeh Oskoueiyan Rabee Izadi Amoli
        Medicinal plants can be a good alternative to antibiotics by producing antimicrobial compounds. The aim of this study was to investigate the antimicrobial effects of Robinia pseudoacacia L. and Phytolacca americana L. ethanolic leaf extract on several nosocomial pathoge More
        Medicinal plants can be a good alternative to antibiotics by producing antimicrobial compounds. The aim of this study was to investigate the antimicrobial effects of Robinia pseudoacacia L. and Phytolacca americana L. ethanolic leaf extract on several nosocomial pathogenic strains. Plant leaves were collected from the forests of Amol city in the spring of 2016. The extract was extracted by Soxhlet extractor method and then their antibacterial activity at concentrations of 6.25, 12.5, 25, 50, 100 and 200 mg/ml on Proteus mirabilis, Proteus vulgaris, Streptococcus sanguinis, and Staphylococcus aureus were studied by disk diffusion and well dilution methods. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. The highest inhibition zone diameter in the both disk diffusion and well dilution methods was found at concentrations of 200 mg/ml of each plant extract. In the disk diffusion method, R. pseudoacacia extract had highest inhibitory effect on P. mirabilis and P. vulgaris with a growth inhibition zone diameter of 24.67 mm, while P. Americana extract had the highest effect on S. aureus with 27.67 mm. In the well dilution method, R. pseudoacacia extract had the highest inhibition zone diameter on P. mirabilis (24.67 mm), whereas P. Americana showed highest inhibition zone diameter on S. aureus (24.3 mm). The MIC and MBC values of R. pseudoacacia extract was determined at 25 and 50 mg/ml concentrations for P. vulgaris, S. aureus and S. sanguis, and 50 and 100 mg/ml for P. mirabilis. The MIC and MBC values of P. Americana extract was 25 and 50 mg/ml for P. mirabilis, S. aureus and S. sanguis, and 50 and 100 mg/ml for P. vulgaris, respectively. Therefore, based on the results R. pseudoacacia and P. Americana ethanolic extracts had acceptable antimicrobial effect, especially at 200 mg/ml, against some important bacterial pathogens. Manuscript profile
      • Open Access Article

        4 - Evaluation of antibacterial effects of some plant extracts and essential oils against Vibrio parahaemolyticus
        Mehdi Raissy Ali Daghaghelelh Mojtaba Alishahi Mehdi Rahimi
        In this study the antibacterial effects of ethanolic extract of eight medicinal plants (Artemisia aucheri, Satureja bachtiarica, Tanacetum parthenium, Cuminum cyminum, Salvia officinalis, Achillea kellalensis, Stachys lavandulifolia, Myrtuscommunis) and the essential oi More
        In this study the antibacterial effects of ethanolic extract of eight medicinal plants (Artemisia aucheri, Satureja bachtiarica, Tanacetum parthenium, Cuminum cyminum, Salvia officinalis, Achillea kellalensis, Stachys lavandulifolia, Myrtuscommunis) and the essential oil of Satureja bachtiarica, Artemisia aucheri, Tanacetum parthenium, Cuminum cyminum, Achillea kellalensis, Myrtuscommunis on Vibrio parahaemolyticus were evaluated. Standard tube dilution method was used for evaluation of Minimum Bactericidal Concentration value (MBC) and Minimum Inhibitory Concentration value (MIC) of each plant. Then inhibitory zone of bacterial growth for each plant was also measured and compared to positive and negative control. The results showed that the extracts of Satureja bachtiarica had the highest antibacterial effects on V. parahaemolyticus with the inhibitory bacterial growth zone of 15 mm. The extract of Tanacetum parthenium and Cuminum cyminum were also effective with the inhibitory bacterial growth zone of 14 and 11.67 mm against V. parahaemolyticus. Other herbal extract showed no antibacterial effects. The results revealed the higher antibacterial effects of essential oils comparing to the extracts and the highest antibacterial effect were belonged to Satureja bachtiarica, Tanacetum partheniumand Cuminum cyminum. It can be concluded that regarding to high antibacterial effect of Satureja bachtiarica, Tanacetum parthenium and Cuminum cyminum, they can be used in elimination of V. parahaemolyticus. Manuscript profile
      • Open Access Article

        5 - Antimicrobial effects of chemical compounds medical smoke from Teucrium polium on Fluorescence microorganisms under laboratory conditions
        Farhang Tirgir Pardis Naderi dehkordi Fateme Malakpoor Ali Kazemi babaheidari
        Using from medicinal smokes has been customary in treatment of many diseases by bacteria in Iran and especially Chaharmahal‌Va‌Bakhtiari province. The aim of this investigation was to study the antimicrobial effects of medicinal smoke of the Teucrium polium on food-born More
        Using from medicinal smokes has been customary in treatment of many diseases by bacteria in Iran and especially Chaharmahal‌Va‌Bakhtiari province. The aim of this investigation was to study the antimicrobial effects of medicinal smoke of the Teucrium polium on food-borne bacteria in vitro condition. Components from an indirect heating of Teucrium polium were extracted in the form of two organic liquid phases of 1 and 2. Plant extract was also extracted using the ethanol solvent. Then, the antimicrobial effects of two organic phases were analyzed against Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus and Escherichia coli bacteria. Minimum inhibitory concentration (MIC) and minimum bacteria concentration (MBC) were determined using the tube dilution method. Statistically significant difference (P <0.05) was seen between the antimicrobial effects of the organic phases of smoke and extract of the Teucrium polium on tested bacteria. Organic phase 1 of smoke at concentration of 2000 ppm had the highest diameter of the growth inhibition zone on tested bacteria. Tested organic phases had the highest antimicrobial effects on the Staphylococcus aureus. Antimicrobial effects of the organic phases on Gram-positive bacteria were higher than Gram-negative. According to the cheap, low cost, high efficiency and safety of the procedure of the smoke production from the Teucrium polium plant and also its higher antimicrobial effects than extraction, using from organic ophase 1 from the smoke condensate of the Teucrium polium plant is recommended to elimination of harmful bacteria and the cause of corruption in food products as well as increasing their shelf-life. Manuscript profile
      • Open Access Article

        6 - Investigating the antimicrobial effects of lemongrass and lavender extracts and essential oils with antibiotics on some food bacteria
        Mehrdad Ataie Kachouei Elham Fakhri fatemeh khodaverdipour
        In recent years, the increase in drug resistance against bacteria and the increased dosage of common drugs and antibiotics, as well as the side effects caused by these drugs, have brought natural factors such as medicinal plants with fewer side effects, which have recei More
        In recent years, the increase in drug resistance against bacteria and the increased dosage of common drugs and antibiotics, as well as the side effects caused by these drugs, have brought natural factors such as medicinal plants with fewer side effects, which have received more attention. increase. For this reason, in this study, we prepared alcoholic extracts and essential oils from lemongrass and lavender plants and then serially diluted them to obtain the minimum growth inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Shigella Flexnerl, Salmonella typhimuriom, Esherichia coli and Staphylococcus aureus bacteria were prepared. Zones of inhibition of bacterial growth were also measured and compared using the disc diffusion method. The results obtained showed that the halo diameter of the essential oil was larger than that of the extract, indicating a higher susceptibility of the sample to the Gram-positive bacterium Staphylococcus aureus. The amount of MIC and MBC in essential oil and extract, respectively, against each of the four bacteria tested was measured by microdilution method, which showed that the concentration of MIC and MBC in essential oil was lower than in extract. Analysis of compounds in each essential oil and extract was performed using a GC-MS device, essential oils contain more compounds than extracts and therefore have more antibiotic properties. Therefore, the results of this study demonstrate that lemongrass and lavender essences and extracts have antibacterial effects. Therefore, they can be used as natural plant products to combat bacterial infections. Manuscript profile