List of Articles Amniotic membrane

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  1 - Determining of non-toxic dose of Fe3O4 magnetic nanoparticles on amniotic membrane stem cells
  neda mousaviniri maryam naseroleslami
  Superparamagnetic iron oxide nanoparticles (SPIONs) are an Important advancement in the field of nanotechnology. They expand the possibilities of noninvasive analysis for tracking of cells and have many useful properties, making them potential candidates for numerous ap More

  Superparamagnetic iron oxide nanoparticles (SPIONs) are an Important advancement in the field of nanotechnology. They expand the possibilities of noninvasive analysis for tracking of cells and have many useful properties, making them potential candidates for numerous applications in medicine. However, the possibility of toxicity in cells is reported by these nanoparticles. The goal of this study was to find a concentration of SPIONs that cant induce intracellular levels of Reactive Oxygen Species. For this purpose for the first time amniotic membrane stem cells were incubated with different concentrations of SPIONs coated with polyethylene glycol (PEG). Then intracellular levels of Reactive Oxygen Species was measured By probe Rhodamine 123 . Our results demonstrate that there was no significant generators of Reactive Oxygen Species in cells in concentration range of 0-100 μg/mL of spions. However, at concentrations higher than 100 μg / ml, the production of Reactive Oxygen Species increased. According to the results nanoparticles used in this study at concentrations ≤100 μg/mL are suitable for tracking of this cells. Manuscript profile
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  2 - Differentiation of amniotic membrane mesenchymal stem cells (hAMSCs) cardiomyocyte on PGS-co-PCL / PGC / PPy / Gelatin scaffold and its effect in the treatment of myocardial ischemia in adult male Wistar rats
  mahsa Ale Ebrahim NASTARAN BAHRAMI nooshin barikrow yasin asadi fateme roholah ali salimi
  10.30495/jdb.2022.1959843.1308
  AbstractIntroduction: The cardiac ischemia and its subsequent failures have a high prevalence and its treatment is dangerous, expensive and ineffective. With the implant of mesenchymal stem cells, researchers hope to replace the dead tissues and reactivate the damaged p More

  AbstractIntroduction: The cardiac ischemia and its subsequent failures have a high prevalence and its treatment is dangerous, expensive and ineffective. With the implant of mesenchymal stem cells, researchers hope to replace the dead tissues and reactivate the damaged parts of the heart. Materials and methods: After the differentiation of mesenchymal stem cells of the amniotic membrane into heart muscle cells, by closing the left anterior descending coronary artery (LAD) for 45 minutes, acute ischemia was induced in male Wistar rats. Then reperfusion was performed in male rats. Then treatment groups, including: differentiated stem cells, stem cells with PGS-co-PCL/PGC/PPy/Gelatin scaffold and scaffold group, were implanted into the damaged heart. After 2 and 4 weeks, echocardiography was performed and angiogenesis was examined in the heart tissue using the immunohistochemical technique.Results and discussion: In treatment groups with mesenchymal cells and scaffolds + mesenchymal cells compared to the control, the amount of cardiac ejection fraction (EF), ejection fraction (FS) and cardiac stroke volume (SV) increases. Also, by examining the growth of VEGF in different groups and the control group, there was a significant increase in the expression of this angiogenic factor in the group receiving the scaffold + mesenchymal cells compared to other groups, which seems to use the PGS-co-PCL/PGC/PPy./Gel scaffold has created enough and suitable space for growth of vessels in the myocardium. Manuscript profile