List of Articles AGS cells

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  1 - study the effect of Ziziphora Extract on Claudin1 Gene Expression in Cancerous Cell line AGS.
  Zahra Deilami Khiabani Sara Nayyeri
  Background & Aims: Claudins are the main structural and functional proteins of tight junctions in epithelial cells. The altered expression patterns of differ­ent claudin members have been demonstrated in a variety of diseases, particularly in cancers.  It h More

  Background & Aims: Claudins are the main structural and functional proteins of tight junctions in epithelial cells. The altered expression patterns of differ­ent claudin members have been demonstrated in a variety of diseases, particularly in cancers.  It has been reported that claudin 1 is overexpressed 22-fold in gastric adenocarcinoma.  Considering the least side effect of herbal extracts, in this study we have evaluated  claudin-1 gene expression in AGS cells treated with ziziphora extract.Materials and Methods: The AGS cells were incubated 37°C containing 5% CO2  with 85% humidity DMEM with  10% FBS .  The cells were treated with concentrations of 800, 1200, 2000 μg /ml of ziziphora for 48 and 72 hours. Extraction of RNA, synthesis of cDNA has been done using kit. The study of claudin-1 gene expression was performed by Real time PCR and also GAPDH gene was used as the internal control. Data analysis was performed with 2 - ΔΔCT and statistically analyzed by T-Test  .Results: The results of Real time PCR data have shown in 48 hours treatment, reduction of 7 and 2.3 fold with concentrations of 800 and 1200 μg /ml of ziziphora  respectively  and  in  72 hours treatment, reduction of 5.8 and 10 fold have seen in concentrations of 800 and 1200μg /ml of ziziphora, respectively and there was no significant  change in concentration of 2000μg / ml in both treatments.Conclusion: Ziziphora extract in 1200 μg /ml concentration, reduced the expression of claudin-1 remarkably. Claudin-1 may become therapeutic targets for cancer treatment and ziziphora  as a herbal extract decreased the expression rate of claudin1significantly, in lower concentrations treatment. Manuscript profile