List of Articles (Eryngium pulegium)

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  1 - Green production of silver nanoparticles from )Eryngium planum( and its antimicrobial effect on )Escherichia coli( and )Staphylococcus aureus(
  zahra khoshhal Atoosa Vaziri rahele rahbarian
  10.30495/zisti.2023.1983554.1158
  Background: The development of nanotechnology in the last decade has opened a new and unique window to science; Among them, silver nanoparticles are one of the most important types of nanoparticles due to their antimicrobial properties. There are different methods for p More

  Background: The development of nanotechnology in the last decade has opened a new and unique window to science; Among them, silver nanoparticles are one of the most important types of nanoparticles due to their antimicrobial properties. There are different methods for producing silver nanoparticles by green synthesis. Using plant extracts to produce silver nanoparticles is a cheap and cost-effective method that does not harm the environment.Objective: In this research, the phytochemistry of the plant and the production of silver nanoparticles using the leaves of )Eryngium pulegium( were studied. Also, the antibacterial effect of the resulting nanoparticles was investigated on Escherichia coli and Staphylococcus aureus bacteria.Methods: First, secondary metabolites including phenol, flavonoids, terpenoid and alkaloids were measured. Then, the amount of antioxidant activity of Chuchag) Eryngium pulegium) plant extract was measured by DPPH method and after confirming the presence of antioxidant property and high amount of secondary metabolites in the examined plant, the synthesis of silver nanoparticle was carried out by aqueous extract of Chuchag plant)Eryngium pulegium( and by spectrophotometry and SEM methods. TEM confirmed the presence of nanoparticles and determined the size and shape of nanoparticles. Then, the antibacterial property of the extract along with nanoparticles was evaluated on two species of bacteria, Escherichia coli and Staphylococcus aureus.Findings: The analysis of secondary metabolites in (Eryngium pulegium) plant showed that the main and indicative compounds in (Eryngium pulegium) essential oil include terpenoids curcumin, gamma-3 carne, beta gorgonen, alpha guain, gamma cadinene, alpha terpinen, etc. The results of LC.MS of alkaloids showed that Eryngium pulegium has 4 alkaloid compounds in order of highest concentration, berbamine, coxoline, balochistin and fikamin. The results related to DPPH in (Eryngium pulegium) plant showed that as the concentration of ( Eryngium pulegium) extract increased, its free radical inhibition percentage also increased, so it can be proved that Eryngium pulegium has free radical inhibitory properties. It also has antioxidant properties. Vis-UV spectroscopic analysis and the presence of a peak at 370 nm indicated the biosynthesis of these nanoparticles in (Eryngium pulegium) extract, and the SEM image determined the shape of the nanoparticles to be spherical and their average size around 20 nm. In the investigation of the antibacterial effect of nanoparticles biosynthesized with (Eryngium pulegium) extract against microbial bacteria, the nanoparticles showed a good antibacterial effect against the studied bacteria. In the MIC test on Staphylococcus aureus bacteria, the minimum inhibitory concentration in(Eryngium Manuscript profile