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  1 - Evaluation of virulence and enterotoxin genes in Salmonella enteritidis strains isolated from ‎Meat and Egg samples by Multiplex-PCR
  Toktam Khodadadipour Kumarss Amini Razagh Mahmoudi
  Today,food borne diseases are one of the most serious problems and occur as a result of consumption to contaminated food and water. The aim of the study was evaluation of virulence and enterotoxin genes in Salmonella enteritidis strains isolated from food samples by mul More

  Today,food borne diseases are one of the most serious problems and occur as a result of consumption to contaminated food and water. The aim of the study was evaluation of virulence and enterotoxin genes in Salmonella enteritidis strains isolated from food samples by multiplex-PCR. This descriptive, cross-sectional study was performed at 2014 on the 1250 no duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of Stn،sopB،slyA،spvc and Phop/Q genes. Sixty Salmonella enteritidis strains were obtained from poultry meat (35 strains, 58.3%) and eggs (25 strains, 41.6%), respectively. molecular analysis distribution showed all isolates (100%) were absence for spvC gene. The highest and lowest prevalence of the genes were related to Phop/Q and SopB,33.3% and 1.6%, respectively. Evaluation of virulence genes and enterotoxin in the Salmonella enteritidis isolated from the food samples are useful because of presence of the genes and efficacy of M-PCR method in epidemiological investigation and assessment of intraspecies genes transfer in food samples. Manuscript profile