Acinetobacter baumannii (A. baumannii) strains with multiple drug resistance are mainly opportunistic pathogens in the development of hospital infections and as an emerging contaminant in livestock-based foods. The aim of this study was to determine the antibiotic resis More
Acinetobacter baumannii (A. baumannii) strains with multiple drug resistance are mainly opportunistic pathogens in the development of hospital infections and as an emerging contaminant in livestock-based foods. The aim of this study was to determine the antibiotic resistance pattern and genotyping of this bacterium strains in raw meat of poultry and livestock. 22 strains isolated from raw meat were tested by multi-locus sequence typing and simple disk diffusion methods. The highest antimicrobial resistance was observed to tetracycline with 90.9% and the least antibiotic resistance was azithromycin and imipenem with 9.09%. Five strains were identified as non-typing isolates in 22 isolates of A. baumannii. Five genetic profiles (Sequence Types=ST) including ST15, ST10, ST12, ST25, ST25 were identified. Identifying the acceptable level of genetic variation among isolates using the MLST technique indicates that this method is considered as a useful method in the study and typing of Acinetobacter spp. strains and can be strains isolated from different origins in different groups. In this study, it was found that by sequencing of house-keeping genes, it is possible to typing of Acinetobacter spp. strains, and this amount of polymorphism indicates that this technique is a useful method for analyzing the genetic diversity of A. baumannii strains is a source of animal origin.
Manuscript profile
Escherichia coli O157:H7 is one of the most important enteric pathogenic strains that is usually transmitted to humans through contaminated water and food. These strains cause hemorrhagic colitis, HUS syndrome, cytopenic purpura thrombosis, and, in some cases, death. Th More
Escherichia coli O157:H7 is one of the most important enteric pathogenic strains that is usually transmitted to humans through contaminated water and food. These strains cause hemorrhagic colitis, HUS syndrome, cytopenic purpura thrombosis, and, in some cases, death. The main repository of these bacteria is ruminants. The aim of this study was genetic classification (genotyping) of isolates of this strain isolated from raw meat and poultry by RAPD-PCR method. 344 samples of ruminants and poultry were collected from fresh meat supply centers in Isfahan and Shahrekord. Genetic classification of O157:H7 isolates was performed by the RAPD-PCR method. Of 344 raw meat samples studied, 202 Escherichia coli isolates (58.7%) were isolated. The rate of O157: H7 strain was 17.8% (36 samples). Genetic classification of Escherichia coli O157:H7 isolates showed 9 different profiles among these 36 isolates and found an affinity of 43.7 to 100% among the isolates. This study showed high molecular similarity between E. coli O157: H7 strains isolated from different animals in Isfahan and Shahrekord. It was also found that RAPD-PCR is a simple, fast, and inexpensive method for describing the genetic diversity of different E. coli strains including strain O157:H7.
Manuscript profile
Pseudomonas aeruginosa strains are considered as the main causes of food spoilage and occasionally foodborne diseases. The present study was performed to evaluate the pattern of antibiotic resistance and frequency of virulence factors in Pseudomonas aeruginosa strains i More
Pseudomonas aeruginosa strains are considered as the main causes of food spoilage and occasionally foodborne diseases. The present study was performed to evaluate the pattern of antibiotic resistance and frequency of virulence factors in Pseudomonas aeruginosa strains isolated from meat. A total of 120 samples of raw and frozen meat were collected. Samples were evaluated for the presence of Pseudomonas aeruginosa using conventional microbial culture. The pattern of antibiotic resistance of Pseudomonas aeruginosa isolates was evaluated using the disk diffusion method. Genomic DNA was extracted from Pseudomonas aeruginosa isolates and the frequency of virulence factors was assessed using the polymerase chain reaction. Eleven out of 120 meat samples (9.16%) were contaminated with Pseudomonas aeruginosa. The prevalence of Pseudomonas aeruginosa in raw and curled meat samples was 5.00 and 13.33%, respectively. There was a statistically significant difference in the prevalence of Pseudomonas aeruginosa between raw and frozen meat samples (P <0.05). Pseudomonas aeruginosa isolates had the highest rate of antibiotic resistance against ampicillin (100%), penicillin (90.90%) and tetracycline (81.81%). The prevalence of resistance to imipenem (9.09%) and trimethoprim (18.18%) antibiotics was lower than other cases. ExoU (54.54%) and exoT (18.18%) genes were the most frequent virulence factors detected. The simultaneous presence of virulence factors and antibiotic resistance in Pseudomonas aeruginosa strains isolated from raw and frozen meat increases the need for further studies to confirm the role of this bacterium as an important food-spoilage bacterium.
Manuscript profile
Sanad
Sanad is a platform for managing Azad University publications
