List of Articles کلون های ژنتیکی

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  1 - Genotyping of Staphylococcus epidermidis strains isolated from nosocomial infections by Multilocus Sequence Typing (MLST)
  Sara Rafiee Elahe Tajbakhsh Hassan Momtaz
  Background & Objectives: Staphylococcus epidermidis is one of the main causes of urinary tract infections and second cause of respiratory infections in human. The aim of this study was genotyping S. epidermidis strains isolated from nosocomial infections and detecti More

  Background & Objectives: Staphylococcus epidermidis is one of the main causes of urinary tract infections and second cause of respiratory infections in human. The aim of this study was genotyping S. epidermidis strains isolated from nosocomial infections and detection of genetic clones using Multilocus Sequence Typing (MLST) method. Materials & Methods: In this cross-sectional study, 16 S. epidermidis isolates were selected and PCR products from amplification of seven housekeeping genes were sequenced. The nucleotide sequences of each gene in each isolate were analyzed in the MLST database and, besides identifying different clones, gene - specific alleles in each sequence types (ST) were determined. Results: A total of 3 clones including ST22, ST88 and ST153 were identified from 16 isolates, which was classified into two gene clusters of A and B. ST22 clone with a frequency of 50%, ST88 with 31.25% and ST153 with 18.75% were identified. The most dominant S. epidermidis clone isolated in 16 isolates is ST22. Conclusion: Dendrogram analysis of the isolates showed the homology of all isolates to alleles previously reported. Furthermore, our results suggest the genetic diversity of the isolates. Manuscript profile
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  2 - Molecular typing of the Acinetobacter baumannii strains isolated from blood infections using Multi Locus Sequence Typing (MLST)
  Zohreh Mohammadi Hassan Momtaz
  Background & Objectives: Acinetobacter baumannii is a Gram-negative coccobacillus that is widely distributed in nature and considered as one of the important causes of hospital infections. The present study was conducted to genotype Acinetobacter baumannii strains i More

  Background & Objectives: Acinetobacter baumannii is a Gram-negative coccobacillus that is widely distributed in nature and considered as one of the important causes of hospital infections. The present study was conducted to genotype Acinetobacter baumannii strains isolated from blood infections using Multi Locus Sequence Typing (MLST) method. Materials & Methods: A total of 36 Acinetobacter baumannii strains were isolated from blood infection samples collected from Baqiatalah and Payambaran hospitals, Tehran, Iran. The PCR products obtained from amplification of seven housekeeping genes were sequenced. The nucleotide sequences of each gene in each isolate were queried against the reference sequence in the MLST database. In addition to characterization of the alleles specific to each gene, thesequence types (ST) of all isolates were determined. Results: A total of 5 clones including ST25, ST136, ST307, ST327, and ST328 were identified in 36 isolates. ST of 2 isolates were not identified in MLST database. The identified STs were placed into 5 genetic clusters including A, B, C, D, and E. Conclusion: Identifying an acceptable level of genetic diversity among the isolates using MLST technique shows that this method is useful for studying and typing of Acinetobacter baumannii isolates. Therefore, it is possible to cluster isolates with diverse origins in different groups. Manuscript profile