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      • Open Access Article

        1 - Screening of resistance genes to Fusarium Crown rot disease in Tomato (Lycopersicon esculentum) cultivars using RAPD markers
        Bahar Morid Shahab Hajmansoor
        Background and Objectives: usarium crown rot caused by F. oxysporum f.sp. radicis-lycopersici is one of reasons of decrease in tomato yield worldwide.. Cultivation of resistant tomato cultivars is the best way to control this disease. Molecular markers linked to resista More
        Background and Objectives: usarium crown rot caused by F. oxysporum f.sp. radicis-lycopersici is one of reasons of decrease in tomato yield worldwide.. Cultivation of resistant tomato cultivars is the best way to control this disease. Molecular markers linked to resistance genes would be useful for improving tomato breeding programs. This study was conducted to identify resistant (existence Frl gene) and sensitive varieties of tomatoes using RAPD marker. Material and Methods: DNA was extracted using CTAB method from 27 tomato varieties. PCR technique was used to identify F. oxysporum f.sp. radicis-lycopersici. Then RAPD marker UBC 194 was used to amplify resistance gene Frl. Results were confirmed by pathogenicity test. Results: In this study, based on UBC 194 marker it was found that 20 out of 27 tomato varieties and hybrids contained a 590 bp index band (existence of resistance gene Frl). Pathogenicity test showed that the tomato varieties that the resistance gene Frl was found in them had no symptoms while the varieties that did not contain the gene showed disease symptoms with different disease index. Conclusion: Plantation of resistant varieties in the infected areas can control this disease without using fungicides. Manuscript profile
      • Open Access Article

        2 - The effect of Rhizobium leguminosarum strains on Fusarium root rot disease of bean
        Maryam Hatamabadi Farahani saeed vezaee mohammadrez lak
        Root rot is one of the most important disease of bean. Management of the disease is difficult because the pathogen is soil borne. In this study the effect of some strains of Rhizobium leguminosarum bacteria on Fusarium root rot of local Khomein chitti bean variety were More
        Root rot is one of the most important disease of bean. Management of the disease is difficult because the pathogen is soil borne. In this study the effect of some strains of Rhizobium leguminosarum bacteria on Fusarium root rot of local Khomein chitti bean variety were evaluated in greenhouse condition. This experiment was conducted with 10 treatments (inoculation with 5 strains of Rhizobium, Rovral-TS fungicide, and healthy and infected control with water and suger-water) based on completely randomized design with four replications during 2007-2008. Results showed that  Rhizobium R-115 had the highest effect on disease severity reduction. This treatment reduced disease severity by 42% compared with infected control. There was no significant difference in disease severity reduction between the other Rhizobium tretments and Rovral-TS fungicide. R-115 increased N concentration (%1.59) compared with %0.74 in infected control. Also all Rhizobium treatments with increasing N concentration, improved plant growth with higher height, dry root and aerial plant weight. Manuscript profile