List of Articles پوسیدگی دندان

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Anti adhesive effect of Lactobacillus rhamnosus as a probiotic on oral Streptococci
  Sanaz Tahmourespour Arezoo Tahmourespour Rooha Kasra Kermanshahi3
  Background & Objectives: Dental caries, caused mainly by mutans Streptococci, is the most common chronic disease in the world. Employment of probiotics is a new emerged technique to prevent dental caries production, and Lactobacillus rhamnosus is one of the importan More

  Background & Objectives: Dental caries, caused mainly by mutans Streptococci, is the most common chronic disease in the world. Employment of probiotics is a new emerged technique to prevent dental caries production, and Lactobacillus rhamnosus is one of the important common probiotics in this strategy. This study aimed to evaluate the effects of L. rhamnosus on the adhesion of oral Streptococci. Materials & Methods: This cross-sectional study was carried out on 40 isolates of mutans and non-mutans Streptococci isolated from dental plaque and caries of the volunteers who admitted in dental school of Islamic Azad University, Khorasgan. The biofilm formation ability of these strains was investigated and the strongest isolates in this term were selected. The effect of L. rhamnosus (ATCC4769) on the adhesion of Streptococci was investigated by several methods; 1: an equal volum of Lactobacillus and Streptococcus, 2: application of Lactobacilli 30 minutes before induction of Streptococci into the system, 3: probiotic pellet 4: a supernatant of the overnight culture of probiotic. Results: Overall, L. rhamnousus led to reduction of Streptococci adhesion to surface using all four strategies. The second method was the most effective, its effect was more efficient on S. mutans adhesion than non-mutans. There was no significant difference between third and fourth methods, but effect of both methods on S. mutans was more than other Streptococci. Conclusion: Application of L. rhamnousus as probiotic could reduce the adhesion of mutans and non-mutans Streptococci to dental surfaces and therefore can reduce dental decay. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Isolation and identification of culturable bacterial flora of dental lesions in a clinic
  Ali Kazemtabrizi Azam Haddadi Mahmoud Shavandi Nasser Harzandi
  10.30495/jmw.2021.690449
  Background & Objectives: Dental plaque is structurally and functionally a biofilm that may lead to caries and root infection due to disruption of microbial homeostasis. The present research aimed to investigate the molecular characteristics of dental flora of patien More

  Background & Objectives: Dental plaque is structurally and functionally a biofilm that may lead to caries and root infection due to disruption of microbial homeostasis. The present research aimed to investigate the molecular characteristics of dental flora of patients in East Tehran. Materials & Methods: In this cross-sectional study, 9 samples of dental plaque, caries, and root canal from five patients who were randomly selected in 2017 were collected under sterile             conditions. Bacteria were cultured using the standard BHI broth culture medium. General primers of the 16S rRNA gene were used for molecular identification of bacteria and investigation of their phylogenetic relationships. Demographic characteristics of the subjects were also examined. Results: Thirteen bacterial isolates were identified from clinical specimens of plaque and tooth decay. The isolated bacteria belonged to three phyla, five families, six genera Arthrobacter,      Brevundimonas, Granulicatella, Kocuria, Neisseria, and Streptococcus, as well as seven species. The most abundant isolates were N. perflava (n=5) and S. salivarius (n=3). The identified bacteria were arranged in four branches of a phylogenetic tree. No association was found between bacteria and demographic characteristics. Conclusion: Identifying the factors involved in dental infections is an effective approach to       prevention. In this study, 11 bacterial isolates from dental plaque and 2 bacterial isolates from tooth decay were identified and no strains from the root specimens were identified. The              discrepancy between plaque bacteria and caries may be due to the small sample size and            microbiological methods used.   Manuscript profile