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Open Access Article
1 - Evaluation of the effect of magnetic field, gibberellic acid hormone and temperature treatments on some physiological characteristics of Salva strawberry in Shiraz region
Mohammad Reza Zandi Abolhossein Aboutalebi Jahromi Behnam Behroznam Abdul Rasool ZakirinIn order to investigate the effect of gibberellic acid, magnetic field and temperature on strawberry, this research was conducted in form of split plot on based of randomiz completely design and treatments include gibberellic acid, magnetic field and temperature stress MoreIn order to investigate the effect of gibberellic acid, magnetic field and temperature on strawberry, this research was conducted in form of split plot on based of randomiz completely design and treatments include gibberellic acid, magnetic field and temperature stress in three levels. The results of analysis of variance of the data showed that the interaction effect on all studied traits was significant. The comparison of the averages showed that the highest amounts of chlorophyll a (14.95 mg of protein per minute) and total chlorophyll (28.54 mg of protein per minute) in the treatment of 100 mg/L gibberellic acid, magnetic field of 10 millitesla and temperature of 2 degrees and the highest of chlorophyll b (9.26 mg of protein per minute) and polyphenol oxidase enzyme (57.52 mg of protein per minute) were obtained in the treatment of 50 mg/L of gibberellic acid, 10 milliTesla magnetic field and 8 degrees. The highest amounts of peroxidase (53.6 mg of protein per minute) in the treatment of 100 mg/liter of gibberellic acid acid, magnetic field of twenty millitesla and temperature of twenty degrees Celsius and the highest values of titratable acidity (0.89 percent) and pH (6.9) were obtained in the treatment of no use of gibberellic acid, no use of magnetic field and temperature of 20 degrees. In terms of the triple interaction effect, the best effective treatment on increasing the storage time of strawberries was the combined treatment of 50 mg/L gibberellic acid and 10 milliTesla magnetic field and 8 degrees Celsius temperature. Manuscript profile -
Open Access Article
2 - The Response of Pot Marigold Plant (Calendula officinalis L.) to Ascorbic Acid and Brassinosteroid under Drought Stress
Khatereh Hemmati Ali Ebadi Saeed Khomari Mohammad SedghiTo evaluate water deficit stress, brassinosteroid and ascorbic acid effects on photosynthetic pigments and some pot marigold’s compatible solutes, an experiment was conducted at the experimental field and labratory of university of Mohaghegh Ardabili in 2014. A fa MoreTo evaluate water deficit stress, brassinosteroid and ascorbic acid effects on photosynthetic pigments and some pot marigold’s compatible solutes, an experiment was conducted at the experimental field and labratory of university of Mohaghegh Ardabili in 2014. A factorial split experiment based on completely randomized design with three replications were used. Water deficit was induced by two levels of water stress (50 and 100 mm evaporation from class A pan) considered as main factor and brassinosteroid (0, 10-8 and 10-7 M) and ascorbic acid (0 and 10 mM) as sub factors. Results showed that water deficit, brassinosteroid and ascorbic acid increased soluble sugars, carotenoids and polyphenol oxidase activity. Water deficit decreased the rate of a, b and total chlorophylls, lysine and methionine. However, application of brassinosteroid and ascorbic acid decreased water deficit effects and increased the rate of a, b and total chlorophylls. Ascorbic acid application under stress condition increased the rate of lysine and methionine. Considering these results it can concluded that ascorbic acid and brassinosteroid increase marigold tolerance to water shortage by enhancing defensive system and prevention of photosynthetic pigments destruction. It seems application of brassinosteroid can improve medicinal particularity of marigold. Manuscript profile -
Open Access Article
3 - Effect of Nano-TiO2 and Salicylic Acid Foliar Application on some Biochemical Traits of Corn 704 Single Cross under Water Regimes
Faezeh Shargi Ebrahim Khalilvand BehrouzyarIn order to investigate the effects of water deficit stress and nano-TiO2 and salicylic acid foliar application on some biochemical traits of corn704 single crossplant, an experiment was conducted in split plot factorial based on RCBD in three replications at the Resear MoreIn order to investigate the effects of water deficit stress and nano-TiO2 and salicylic acid foliar application on some biochemical traits of corn704 single crossplant, an experiment was conducted in split plot factorial based on RCBD in three replications at the Research Station of the Islamic Azad University, Tabriz Branch, during growing seasons of 2017-2018. Treatments were water deficit stress in three levels contained: 50, 75 and 100% filed capacity (FC) as well as thefactorial combination of nano-TiO2 (n-TiO2) foliar application in three levels contains: non application (control), 0.01 and 0.03 and salicylic acid (SA) foliar application in two levels contain: non application and 0.5%. Result showed that 0.5% SA foliar application under 50% water deficit stress had the highest peroxidase, polyphenol oxidase and lowest malondialdehyde activity. Furthmore, 0.01% n-TiO2 foliar application had the highest effect on peroxidase and lowest effect on malondialdehyde activity. Use and non-use condition of SA, 0.01% n-TiO2 foliar application had the positive effect on polyphenol oxidase activity. Foliar application of 0.01% n-TiO2 and0.5% SA under water deficit stress had the highest effect on soluble carbohydrates and proline. Based on the results, the guaiacol peroxidase enzyme activity increased with increasing water deficit stress level, so that the most activity of this enzyme was observed in irrigation with 50 % filed capacity. Considering the role of the traits studied in cooperation with each other in preventing the production of active oxygen species and reducing the effects of water deficit stress, SA and n-TiO2, increasing the activity of enzyme peroxidase, polyphenol oxidase, soluble carbohydrates, Proline and in contrast to the reduction of malondialdehyde reduced the negative effects of water deficit stress. Since SA and n-TiO2 had positive effects on the enzymes affecting plant physiology, the seed yield increased significantly compared to control and water deficiet conditions. Manuscript profile -
Open Access Article
4 - Effect of aqueous extract of canola (Brassica napus L. cv RGS) on germination, growth and antioxidant enzymes activity in weed of Phallaris minor seedling
حسین LariYazdi مه لقا Ghorbanli میترا RoshaniAllelopathy is phenomenone that some of the plant species relase special chemical compounds and by this way inhibit germination and growth in other plants.Today using of allelopathic potential in plant is considering in order to control of weeds growth. It is said MoreAllelopathy is phenomenone that some of the plant species relase special chemical compounds and by this way inhibit germination and growth in other plants.Today using of allelopathic potential in plant is considering in order to control of weeds growth. It is said that allelochemical compounds may in lower concentrations cause stimulate of growth and in higher concentrations inhibit it.In this research effect of different concentrations of aqueous extract of canola (0,10,20,40,60,80%) on germination percentage, growth parameters and peroxidase, catalase and polyphenol oxidase activity in Phallaris minor seedling was evaluated. Results this research showed that canola extract in higher concentrations decreased germination percentage, length, fresh and dry weight in radicle and hypocotyl of Phallaris.Also activity of antioxidant enzymes as peroxidase and catalase in higher concentrations of extract decreased.Only in 20% concentration of canola extract germination ,fresh weight of radicle, hypocotyl length and dry weight hypocotyl in Phallaris increased. Manuscript profile -
Open Access Article
5 - Effect of drought stress and ascorbic acid on some of antioxidant enzymes in antioxidative defense in Pimpinella anisum L.
zh Asadi kavan مه لقا Ghorbanli, آرین SateeiDrought stress is one of the abiotic stresses that induces reactive oxygen species(ROS) formation in plant tissues. ROS signaling promotes complex networks of antioxidant enzymes. Ascorbic acid is an important buffer protects cellular structures against oxidative attack MoreDrought stress is one of the abiotic stresses that induces reactive oxygen species(ROS) formation in plant tissues. ROS signaling promotes complex networks of antioxidant enzymes. Ascorbic acid is an important buffer protects cellular structures against oxidative attack in plants. Effect of drought stress on catalase (CAT), ascorbate peroxidase (APX) and polyphenol oxidase (PPO) enzyme activities was evaluated in the presence and absence of ascorbate in herbal aromatic plant Pimpinella anisum L. with great value of export that exposed to controlled water deficit. This study was done in flower pot condition. Biochemical and physiological properties measured randomly in control groups and treatments (with drought 60% and 25% of field capacity and ascorbate with concentration of 1.4 mM). Results showed that with increasing stress levels in leaves CAT, APX and PPO enzyme activities increased significantly in the presence and absence of ascorbate. Activity of root CAT and APX with and without ascorbate at the level of drought 60% increased significantly and at the level 25% of field capacity decreased to the level of control. However ascorbate treatment increased significantly the root enzymatic activities in whole stress levels. Manuscript profile -
Open Access Article
6 - بررسی اثر تنش خشکی بر میزان پرولین، قندهای محلول و فعالیت آنزیمهای آنتیاکسیدانی گیاه داروییL. Oenothera biennis
مریم رجبی مه لقا قربانلی علی جعفری مفید آبادی -
Open Access Article
7 - Optimization of Polyphenol Oxidase and Peroxidase Production Using Native Bacillus spp. Isolated from Fully Fermented Tea
Moahmmad Faezi GhasemiTea (Camellia sinensis) is a species of evergreen shrub or small tree in the flowering plant family of Thecae. One of the major steps in black tea manufacturing is the fermentation process, which bacteria may incorporate. This study aimed at using the one-factor-at-a-ti MoreTea (Camellia sinensis) is a species of evergreen shrub or small tree in the flowering plant family of Thecae. One of the major steps in black tea manufacturing is the fermentation process, which bacteria may incorporate. This study aimed at using the one-factor-at-a-time (OFAT) and Taguchi methods to optimize the production of polyphenol oxidase (PPO) and peroxidase (POD) by Bacillus spp. isolated from fully fermented tea. The results showed that sucrose and glucose exhibited the greatest effects on the production of polyphenol oxidase and peroxidase by the strains Bacillus sp. (TB3) and B. licheniformis (TB14), respectively. In addition, the results indicated that the best nitrogen sources for the production of PPO by Bacillus sp. (TB3) and POD by B. licheniformis (TB14) were sodium nitrate and ammonium carbonate, respectively. Based on the obtained results, the medium components for the production of PPO by Bacillus sp. (TB3) at a pH of 7 were as follows: 0.5% sucrose, 1.0% of peptone, 0.8% of yeast extract, 0.2% of hydrolyzed casein, 0.02% of potassium dihydrogen phosphate, 0.005% of magnesium sulfate heptahydrate, and 0.1% sodium nitrate. Moreover, the optimal culture medium for the production of POD by B. licheniformis (TB14) at a pH of 7 was as follows: 0.3% of glucose, 1.0% of peptone, 0.8% of yeast extract, 0.2% of hydrolyzed casein, 0.02% of potassium dihydrogen phosphate, and 0.005% of magnesium sulfate heptahydrate. Increased production of PPO and POD enzymes were obtained about 8 and 6 fold more than the basal culture media, respectively. Manuscript profile -
Open Access Article
8 - تاثیر تابش نور آبی و غلظت های مختلف نانوسیلور بر عمر گلجایی و صفات دخیل بر حفظ کیفیت پس از برداشت گلهای شاخه بریده آلسترومریا رقم ’ناپولی‘
مهرآسا انوری داود هاشم آبادی بهزاد کاویانی لیلا اسدپورکاربرد نور آبی در مرحله پس از برداشت گل­های شاخه بریده روشی جدید جهت بهبود عمر گلجایی است. از این­رو پژوهش حاضر با هدف بررسی اثر مدت زمان­های مختلف تابش نور آبی (6، 12، 18 و 24 ساعت) و تیمار پالس نانوسیلور (5، 10، 15 و 20 میلی­گرم در لیتر) بر عمر گ Moreکاربرد نور آبی در مرحله پس از برداشت گل­های شاخه بریده روشی جدید جهت بهبود عمر گلجایی است. از این­رو پژوهش حاضر با هدف بررسی اثر مدت زمان­های مختلف تابش نور آبی (6، 12، 18 و 24 ساعت) و تیمار پالس نانوسیلور (5، 10، 15 و 20 میلی­گرم در لیتر) بر عمر گلجایی و صفات وابسته به آن در گل­های شاخه بریده آلسترومریا رقم ’ناپولی‘ اجرا شد. از ساکارز 3 درصد به­عنوان تیمار شاهد استفاده شد. این آزمایش در قالب طرح کاملا تصادفی در سه تکرار اجرا شد. نتایج نشان داد که بیشترین عمر گلجایی متعلق به تیمارهای 10 میلی­گرم در لیتر نانوسیلور (66/22 روز) است که از نظر آماری با تیمارهای 12 ساعت نور آبی (00/22 روز) و 20 میلی­گرم در لیتر نانوسیلور (66/21 روز) تفاوت معناداری نداشت. کمترین کاهش وزن تر به­ترتیب با کاربرد 18 و 12ساعت نور آبی و 10 میلی­گرم در لیتر نانوسیلور بدست آمد. بیشترین درصد ماده خشک (34/57 درصد) با کاربرد 10 میلی­گرم در لیتر نانوسیلور بدست آمد. گل­هایی که به مدت 12 ساعت با نور آبی تیمار شدند دارای بیشترین میزان کلروفیل a و b (به­ترتیب با 52/2 و 27/1 میلی­گرم در هر گرم وزن تر)، بیشترین فعالیت آنزیم کاتالاز (26/5 نانومول در هر گرم وزن تر در دقیقه) و کمترین فعالیت آنزیم پلی­فنل اکسیداز (007/0 میکرومول در هر گرم وزن تر در دقیقه) بودند. کمترین باکتری محلول گلجایی با کاربرد 15 میلی­گرم در لیتر نانوسیلور و بیشترین پروتئین گلبرگ با کاربرد 24 ساعت نور آبی حاصل شد. نانوسیلور در کنترل و مهار باکتری­های گرم منفی و نور آبی در کنترل و مهار باکتری­های گرم مثبت در محلول گلجایی موثر بودند. با توجه به نتایج می­توان نور آبی را به­عنوان یک عامل فیزیکی موثر در حفظ عمر گلجایی و صفات وابسته به آن در گل­های شاخه بریده آلسترومریا رقم ’ناپولی‘ معرفی کرد. Manuscript profile -
Open Access Article
9 - پرولین و آرژنین با بهبود برخی از پارامترهای فیزیکوشیمیایی، عمر پس از برداشت گل های شاخه بریده آلسترومریا ’مارس‘ را افزایش می دهند
دینا یعقوبی کیاسه داود هاشم آبادی بهزاد کاویانیبه­منظور بررسی اثر سیکلوهگزماید و اسیدهای آمینه آرژنین و پرولین بر ماندگاری پس از برداشت گل­های شاخه بریده آلسترومریا رقم ’مارس‘ آزمایشی فاکتوریل در قالب طرح کاملا تصادفی در سه تکرار با 20 تیمار اجرا شد. در این آزمایش از دو فاکتور شامل سیکلوهگزماید ( Moreبه­منظور بررسی اثر سیکلوهگزماید و اسیدهای آمینه آرژنین و پرولین بر ماندگاری پس از برداشت گل­های شاخه بریده آلسترومریا رقم ’مارس‘ آزمایشی فاکتوریل در قالب طرح کاملا تصادفی در سه تکرار با 20 تیمار اجرا شد. در این آزمایش از دو فاکتور شامل سیکلوهگزماید (CHI) (0، 50، 100 و 1000 میکرومولار) و اسید آمینه (AA) ]0، 5 و 10 میلی­مولار آرژنین (Arg) و 5 و 10 میلی­مولار پرولین (Pro)[ به­صورت تیمار پالس 24 ساعته استفاده شد. نتایج نشان داد که گل­های تیمار شده با "CHI50×Pro10" بیشترین عمر گلجایی (3/9 روز)، بیشترین جذب آب (771/1 میلی­لیتر در هر گرم وزن تر)، بیشترین ماده خشک (56/13 درصد)، کمترین تجمع مالون­دی آلدهید (32/1 نانومول در هر گرم وزن تر) و بیشترین فعالیت آنزیم کاتالاز (68/5 نانومول در هر گرم وزن تر در دقیقه) را داشتند. تیمار "CHI0×Arg5" در حفظ جذب آب، وزن تر، ماده خشک، پروتئین کل، کاهش تجمع مالون­دی­آلدهید و افزایش فعالیت آنزیم سوپراکسید دیسموتاز از موفق­ترین تیمارها بود و رتبه دوم را در افزایش عمر گلجایی (08/9 روز) بخود اختصاص داد. بنابراین دو تیمار "CHI50×Pro10" و "CHI0×Arg5" به­عنوان موفق­­ترین تیمارها در بهبود صفات کمی و کیفی گل شاخه بریده آلسترومریا معرفی می­شوند. کمترین اثر مثبت در اکثر صفات ارزیابی شده متعلق به تیمار 1000 میکرومولار سیکلوهگزماید بود. بنابراین کاربرد سطوح بالای سیکلوهگزماید (1000 میکرومولار) در محلول گلجایی گل­های شاخه بریده آلسترومریا رقم ’مارس‘ به دلیل اثر منفی و سمی بر شاخص­های پس از برداشت توصیه نمی­شود. Manuscript profile -
Open Access Article
10 - Effect of spermidine and application on internal polyamine content, free radical enzyme activity and lipid oxidative destruction in cut ‘Black Magic’ rose flowers.
REza Namatolah Sani Seyed Hosein Nemati Mahmoud Shour Mohammad Farjadi-ShakibRose is one of the most important ornamental plants which nowadays holds the first top position in production and export of cut flowers due to its beautiful and colorful flowers. The quantity and quality of rose cultivars depend on environmental and nutrition. Pre and p MoreRose is one of the most important ornamental plants which nowadays holds the first top position in production and export of cut flowers due to its beautiful and colorful flowers. The quantity and quality of rose cultivars depend on environmental and nutrition. Pre and post-harvest application of plant growth regulators is one of the common methods for quality improvement of this flower. Therefore, in the present study, cut ‘Black Magic’ rose flowers were treated at pre harvest with spermidine (0, 50 and 100 mgl-1) and with salicylic acid (0, 50 and 100 mgl-1) during postharvest. Consequently, postharvest quality from various point of view such as internal polyamine content, activity of free radical enzymes (such as superoxide dismutase, catalase and polyphenol oxidase) and destructive lipid oxidation (malondialdehyde content) were studied. Results indicate that foliar application with spermidine and with salicylic acid increases petal soluble protein content, improves the activity of superoxide dismutase, catalase and poly phenol oxidase in the petals of cut ‘Black Magic’ rose flowers. Activity increment of enzymes that are involved in oxidative stress, decreased lipid peroxidation and consequently, they decreased malondialdehyde content as destructive biomarker. Internal polyamines such as spermine, spermidine and putrescine were also significantly affected by spermidine and salicylic acid application. Manuscript profile
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