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      • Open Access Article

        1 - Use of Potato Waste for Pigments Production by Monascus purpureus in Submerged Cultivation
        F. Kamali M. Jahadi E. Khosravi N. Ghasemisepro
        Introduction: Among the pigment-producing microorganisms, Monascus purpureus is important for controlling diabetes and preventing and reducing fat due to the production of red and orange pigments with therapeutic properties such as anti-cancer, anti-inflammatory. & More
        Introduction: Among the pigment-producing microorganisms, Monascus purpureus is important for controlling diabetes and preventing and reducing fat due to the production of red and orange pigments with therapeutic properties such as anti-cancer, anti-inflammatory.  Materials and Methods: In this study, the effect of replacement of potato starch waste (RPSW) (20-100%), sodium chloride (6-14 g) and thermal stress time of spore suspension (TST) (50-90 seconds) on the growth of M. purpureus and production of red and orange pigments at 30 ° C in submerge culture were studied by the central composite design at 95% confidence level. Results: The results showed that increasing the RPSW by up to 50% significantly reduced red and orange pigments and increased biomass production (p <0.05). On the other hand, increasing the TST of spore suspension to 73 seconds and increasing the amount of sodium chloride at the concentrations above 9 g / l significantly increased the production of biomass and red and orange pigments (p <0.05). At the two optimal point with the RPSW (20.3, 42.5) (%), TDT spore suspension (65, 81)(s) and concentration of sodium chloride (12, 6) (g/l) respectively, the biomass (g / l), red and orange pigments (OD/l) (5.24, 4.55 and 3.5) and (4.55, 4.37 and 5.4), respectively, were obtained.Conclusion: The results of this study showed that since the replacement of potato starch waste increased biomass production but reduced the production of red and orange pigments Monascus purpureus, its amount should be used optimally in culture to achieve the best productivity. Manuscript profile
      • Open Access Article

        2 - Evaluation of Incubation Time, Salt and Date Waste in Production of Orange Pigment by Monascus purpureus Using Response Surface Methodology
        Mahshid Asghari Mahshid Jahadi Nafiseh Ghasemisepro
        Monacus purpureus (M. purpureus)produce six types of pigments from yellow to dark red color pigments. Monascus­ fungus has been welcomed due to its various pigment production and healing properties. The aim of this study, evaluation of incubation time (11-21days), s More
        Monacus purpureus (M. purpureus)produce six types of pigments from yellow to dark red color pigments. Monascus­ fungus has been welcomed due to its various pigment production and healing properties. The aim of this study, evaluation of incubation time (11-21days), salt (7-12 %) and date waste (15-55%) in production of orange pigment by M. purpureus using response surface methodology in solid state. After optimization of these factors, it was showed the highest amount of orange pigment was obtained during the 21-day incubation period, 7% salt and the date syrup concentration was 55%. In optimum conditions, a maximum yield of orange pigment was 5.31 (ODU.ml-1), fermentation yield was 0.252 (ODU.ml-1.day-1), pigment production yield per biomass 0.623 (ODU.mg-1), yield of orange production per carbon source consumption 0.043 (ODU.gr- 1), the substrate conversion rate was 93 (%). The results of this study showed that date waste and Wheat Straw could be used as an affordable and effective substrate for the production of orange pigment by M. purpureus fungus. Manuscript profile
      • Open Access Article

        3 - Optimization of the Orang Pigment Production by monascus purpureus from Date Waste Using Response Surface Methodology
        Fatemeh Bakhshi Mahshid Jahadi
        Pigments produced by Monascus spp. can be used as food grade biocolorant and are preferred over the synthetic variants which elicit various adverse effects. Monascus purpureus PTCC 5303 has been investigated in the present study for orang pigment production employing su More
        Pigments produced by Monascus spp. can be used as food grade biocolorant and are preferred over the synthetic variants which elicit various adverse effects. Monascus purpureus PTCC 5303 has been investigated in the present study for orang pigment production employing submerged fermentation. Central composite design (CCD) of response surface methodology (RSM) was used to optimize the three significant medium components (date sugar concentration (20-60 g/l), salt (6-12%) and pH (6-9)). According to the response surface point prediction analysis, date sugar concentration of 20 g/l, NaCl 6% with 9 pH of medium could give maximum orang pigment yield up to 6.7 OD. Under optimized experimental conditions, a maximum yield of orang pigment, biomass productivity, substrate conversion and productivity of orang pigment production were 6.24±0.05 (OD),7.2 (g/l), 82 (%) and 5.36 (g/l.day), respectively. From the results of this study, date waste can be used as a low cost substrate for the production of orang pigment in large scale studies. Manuscript profile