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      • Open Access Article

        1 - Effect of salicylic acid in alleviating Ni-induced oxidative stress in wheat (Triticum aestivum L.) at germination stage
        A.R Jafari, R.A Khavarinejad حمید Fahimi
        The effects of salicylic acid (SA) in alleviating Ni-induced oxidative stress in wheat (Triticum aestivum L.) at germination stage were studied and the changes in radicle and plumule growth (fresh and dry weight and their lengths) were investigated. Exposure to 800&micr More
        The effects of salicylic acid (SA) in alleviating Ni-induced oxidative stress in wheat (Triticum aestivum L.) at germination stage were studied and the changes in radicle and plumule growth (fresh and dry weight and their lengths) were investigated. Exposure to 800µM Ni significantly decreased radicle and plumule growth, and activities of catalase (CAT) and peroxidase (POD) enzymes but increased the concentration of malondialdehyde (MDA). However, treatment with 100µM SA enhanced the activities of these enzymes in plumule tissues but lowered the concentration of MDA in the Ni-stressed wheat compared with the Ni treatment alone. Treatment with SA alleviated the Ni-induced inhibition of radicle and plumule growth.The results showed that treatment with SA enhanced the antioxidant defense activities in Ni-stressed wheat, thus alleviating Ni-induced oxidative damage and enhancing Ni tolerance. Manuscript profile
      • Open Access Article

        2 - essential elements content in spring canola seedlings cv. PF under nickel stress
        Nader Kazemi Ramazan Ali Khavari-Nejad Taher Nejad-Sattari
        This research was performed in order to investigate the effect of salicylic acid and sodium nitroprusside on growth and essential elements content in spring canola under nickel stress in laboratory of Science and Research Branch, Islamic Azad University of Tehran. The i More
        This research was performed in order to investigate the effect of salicylic acid and sodium nitroprusside on growth and essential elements content in spring canola under nickel stress in laboratory of Science and Research Branch, Islamic Azad University of Tehran. The interactive effects of nickel, salicylic acid (SA) and sodium nitroprusside (SNP) as a donor of nitric oxide (NO) were examined on canola (Brassica napus L. cv. PF) growth. 21-day old canola seedlings (cv. PF) were exposed to different concentrations of NiCl2 , 6H2O (0, 0.5 mM), SA (0, 0.2 mM) and SNP (0, 0.2 mM) for 10 days. Nickel toxicity symptoms such as chlorosis and necrosis were observed on leaves of Ni-treated seedlings. Treatment with Ni resulted in a decrease in fresh and dry weight of roots and shoots. Mineral elements content (Mg, Fe, Ca, P, K) extremely decreased in roots and shoots of Ni-stressed canola seedlings, while the content of N in these seedlings increased in roots and decreased in shoots. Ni was more accumulated in roots than in shoots. In Ni-stressed seedlings, application of SA or NO, especially SA+NO, improved the growth and decreased the toxicity symptoms as compared to Ni-treated seedlings. SA or NO, especially both together, considerably reduced root-to-shoot translocation of Ni and increased the content of mineral elements in roots and shoots of Ni-stressed seedlings. These results showed that SA or NO and in particular their combination, markedly reduced the toxic effects of nickel on canola seedlings by sequestration of Ni in roots and amelioration of mineral nutrition.                                                                                                    Manuscript profile