List of Articles تیپ آمیزشی

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  1 - Study of pathogenicity and determination of mating type of Fusarium proliferatum isolates
  Sepideh Karimi Zanjani Asl Vahid Rahjoo Majid Zamani
  Fusarium proliferatum is the dominant agent of maize fusarium ear rot in many areas of the world especially in Iran. Among several Fusarium isolates recovered from infected maize ears, 12 isolates were identified as F. proliferatum according to morphological and physiol More

  Fusarium proliferatum is the dominant agent of maize fusarium ear rot in many areas of the world especially in Iran. Among several Fusarium isolates recovered from infected maize ears, 12 isolates were identified as F. proliferatum according to morphological and physiological characteristics. In order to determine mating population and mating type of isolates, all 12 isolates were crossed with standard tester isolates of F. proliferatum on Carrot Agar (CA) media and were incubated under the mixture of cool-white and near-UV lights for three to four weeks. This test was repeated twice with three replications. Morphological identification of all isolates was confirmed by mating population test so that all 12 isolates of F. proliferatum belonged to mating population D of Gibberella fujikuroi complex. The results of mating type test showed that seven isolates were MAT-1 (58.3% frequency) and five isolates were MAT-2 (47.7% frequency). Pathogenicity test on stems of susceptible maize line (MO17) was done using toothpicks method in field, greenhouse and laboratory (Detached stem). The Pathogenicity of isolates was evaluated by measuring the necrosis length on inoculated stem. The results of variance analysis of pathogenicity tests showed significant differences among isolates in all mentioned methods. In field, greenhouse and laboratory trials, isolates SK27, SK32 and SK24 had the highest diseases severity (length necrosis), respectively. Significant and positive correlations were observed between field and greenhouse tests (r = 0.86**), field and  laboratory tests (r = 0.75**), and greenhouse and laboratory tests (r = 0.75**). Manuscript profile
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  2 - Frequency of mating type alleles in Alternaria alternata isolated from citrus products collected from South of Iran based on PCR
  Alireza Niazmand Shahab Hajmansoor
  Background & Objectives: Sexual stage of Alternaria alternata has not been discovered yet. However, high levels of phenotypic and genetic diversity have been reported in different populations of A. alternata. In other Ascomycetes similar to this species, sexual repr More

  Background & Objectives: Sexual stage of Alternaria alternata has not been discovered yet. However, high levels of phenotypic and genetic diversity have been reported in different populations of A. alternata. In other Ascomycetes similar to this species, sexual reproduction is controlled by a single locus, named MAT1, with two alleles MAT1-1 and MAT 1-2. The aims of this research were identification of frequency and distribution of both alleles of MAT1in A. alternata population of citrus collected from South of Iran. Materials & Methods: In this cross-sectional study, DNA was extracted from young mycelia of 45 isolates of A. alternata that previously were collected and isolated directly from different species and cultivars of the citrus plants showing leaf spot disease symptoms in different orchards of Southern region of Iran using CTAB method. Mating types were determined using specific primer sets for MAT1-1 and MAT1-2 idiomorphs and PCR assay. Results: A 642 bp amplicon and a 882 bp amplicon were amplified pecificlly for Mat1-1Mat1-2 isolates, respectively. No equal frequency of both mating type idiomorphs were observed in studied samples. Totally 16 and 29 isolates showed the presence of MAT1-1 and MAT1-2 idiomorphs, respectively. Unequal frequency of both idiomorphs was observed in the citrus species and cultivars collected from different regions. Conclusion: It seems that unknown factors are involved in sexual induction of A. alternata in some regions and citrus hosts in South of Iran. Manuscript profile