List of Articles تولید مثل جنسی

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Frequency of mating type alleles in Alternaria alternata isolated from citrus products collected from South of Iran based on PCR
  Alireza Niazmand Shahab Hajmansoor
  Background & Objectives: Sexual stage of Alternaria alternata has not been discovered yet. However, high levels of phenotypic and genetic diversity have been reported in different populations of A. alternata. In other Ascomycetes similar to this species, sexual repr More

  Background & Objectives: Sexual stage of Alternaria alternata has not been discovered yet. However, high levels of phenotypic and genetic diversity have been reported in different populations of A. alternata. In other Ascomycetes similar to this species, sexual reproduction is controlled by a single locus, named MAT1, with two alleles MAT1-1 and MAT 1-2. The aims of this research were identification of frequency and distribution of both alleles of MAT1in A. alternata population of citrus collected from South of Iran. Materials & Methods: In this cross-sectional study, DNA was extracted from young mycelia of 45 isolates of A. alternata that previously were collected and isolated directly from different species and cultivars of the citrus plants showing leaf spot disease symptoms in different orchards of Southern region of Iran using CTAB method. Mating types were determined using specific primer sets for MAT1-1 and MAT1-2 idiomorphs and PCR assay. Results: A 642 bp amplicon and a 882 bp amplicon were amplified pecificlly for Mat1-1Mat1-2 isolates, respectively. No equal frequency of both mating type idiomorphs were observed in studied samples. Totally 16 and 29 isolates showed the presence of MAT1-1 and MAT1-2 idiomorphs, respectively. Unequal frequency of both idiomorphs was observed in the citrus species and cultivars collected from different regions. Conclusion: It seems that unknown factors are involved in sexual induction of A. alternata in some regions and citrus hosts in South of Iran. Manuscript profile