List of Articles بسته‌بندی فعال

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  1 - Active and intelligent food packaging with the use of anti-bacterial and natural-identifier agents
  somayeh mahmoodi eskandar abadi mehdi mahmoodian kaveh rahmani farah
  The main purpose of food packaging is to prevent it from being destroyed by physical, chemical and microbial contamination. A new strategy that has recently been considered for this aim is the use of smart-active food packaging. In this study, ethylene-vinyl acetate cop More

  The main purpose of food packaging is to prevent it from being destroyed by physical, chemical and microbial contamination. A new strategy that has recently been considered for this aim is the use of smart-active food packaging. In this study, ethylene-vinyl acetate copolymer (EVA) was used as an abundant, flexible, and non-toxic polymer for the preparation of packaging films. Anthocyanin extracted from red cabbage was stabilized on the montmorillonite and was added to the film as a time-temperature indicator. Furthermore, other materials such as ZnO and rosemary essential oil and modified montmorillonite were used in order to achieve antimicrobial, antioxidant and air barrier activity in the fabricated smart-active food packaging films. The nanoparticles and prepared films were characterized by XRD and FESEM analysis. The performance of the prepared films were investigated by antioxidant capacity test by DPPH, in vitro test, minimum bactericidal concentration (MBC), minimum inhibition concentration (MIC), antibacterial test and capacity of oxygen absorbance. Results showed that those film which contained additives such as rosemary, ZnO, and modified montmorillonite exhibited remarkable antibacterial and antioxidant activity compared to pristine EVA film. Manuscript profile
• Open Access Article
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  2 - In vitro production of recombinant ergokonin protein and its application in the polylactic acid film to control Aspergillus niger and Penicillium notatum
  Mohammad javad Akbarian Meymand Arash Babaei
  Background & Objectives: Fungal is one of the most important causes of food spoilage. The use of antimicrobial active packaging increases food safety and shelf life. Hence, this study aimed to produce antifungal compounds (Ergoconin) and its application in the polyl More

  Background & Objectives: Fungal is one of the most important causes of food spoilage. The use of antimicrobial active packaging increases food safety and shelf life. Hence, this study aimed to produce antifungal compounds (Ergoconin) and its application in the polylactic acid film to reduce the fungal contamination of Aspergillus niger and Penicillium notatum.  Materials & Methods: To produce the Ergoconin recombinant protein, the target gene was entered into the pET21 expression vector, and the recombinant vector was transferred to Escherichia coli BL21 (DE3) as host of expression. Gene expression was evaluated by SDS-PAGE, western blot and dot blot. To purify the recombinant protein, a chromatography column was used. Poly-lactic acid bioactive films were prepared by casting method and adding different amounts of Ergoconin (0, 0.25, 0.5, and 0.75%) to 4% wt Poly-lactic acid solution. To investigate the antifungal effect of the poly-lactic acid film, Disc diffusion was used.   Results: Ergokonin gene was replicated by specific primers. The expression of recombinant protein was performed by SDS-PAGE and confirmed by dot and western blot. Ergoconin compound had an antifungal effect on both fungi. The inhibition zone diameter for both fungi increased with increasing Ergoconin concentration. The antimicrobial film had a more antifungal effect on Penicillium notatum.   Conclusion: Ergoconin destroys fungi by preventing the synthesis of glucan in the wall of fungi. Therefore, Poly-lactic acid film containing Ergoconin can be used as antimicrobial active packaging to increase food safety. Manuscript profile