List of Articles باکتری‌های بومی

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  1 - Ammonia removal from effluent using isolated native bacteria from Kermanshah Petrochemical Industries effluent
  Mehdi Gowdini Hatam Godini Farhad Salimi
  10.22034/jest.2017.11068
  Background and purpose: Ammonia-Nitrogen is considered one of the environmental pollutants, these pollutants have high concentrations in petrochemical effluent specially, Ammonia and Urea plants. In this study, biological methods using native bacteria consertium isolate More

  Background and purpose: Ammonia-Nitrogen is considered one of the environmental pollutants, these pollutants have high concentrations in petrochemical effluent specially, Ammonia and Urea plants. In this study, biological methods using native bacteria consertium isolated from petrochemical industry has been used for nitrate and ammonia removal. Methods: This study has been carreid out in batch mode and the effects of initial concentrations; pH and retention time has been investigated. The initial concentrations of 3×108 CFU/ml of nitrifier and denitrifier bacteria has been used. An initial ammonia and nitrate concentrations of 50-200 mg/l         as well as the retention time 3-168 hours and  also pH 5,6,7,8 and 9 were studied. Finding: The results showed that the native bacteria isoated from petrochemical industry with a population of 3×108 CFU/ml in pH 8 were able to remove ammonia and nitrate with a initial concentrations of 200 mg/l with 99.5% efficiency in a retention time 4 days for nitrifier, and 6 days for denitrifier bacteria. Discussion and conclusion: The isolated native bacteria had a powerful effect in the nitrification and denitrification processes, so effluent ammonia can be removed with high efficiency by isolated native bacteria, and needs for environmental standards can be applied for effluent discharge.  Manuscript profile
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  2 - Screening Native L-glutaminase Producing Bacteria and Enzyme Production by Submerged Fermentation
  Maryam Ranjbar-Mobarake Mohsen Mobini-Dehkordi Ali Asghar Rastegary
  Background and Objective: L-glutaminase is widely used in pharmaceutical and food industries, such as treatment of leukemia. This study aimed to isolate and identify native L-glutaminase producing bacteria in Iran and to study its quantitative activity in submerged ferm More

  Background and Objective: L-glutaminase is widely used in pharmaceutical and food industries, such as treatment of leukemia. This study aimed to isolate and identify native L-glutaminase producing bacteria in Iran and to study its quantitative activity in submerged fermentation. Material and Methods: The L-glutaminase producer bacteria were isolated from soil and water samples of Zayandehrod and Karoon Rivers and also from the sediments of fishing pools. The isolates were screened using minimal glutamine agar (MGA) medium for producing L-glutaminase enzyme. The samples were grown in a submerged fermentation system using mineral salt glutamine (MSG) medium, and the produced enzyme was assayed quantitatively. The best glutaminase producer bacterium was identified by the gram-staining and biochemical tests. Result: In this study, 11 glutaminase producer strains were isolated. Bacillus subtilis CH3-GLU strain showed maximum enzyme productivity 37.91±0.62 U/ml at temperature 45° C after 96 hours. Conclusion: Based on this study, Bacillus subtilis CH3-GLU showed the highest rate of glutaminase production. Manuscript profile