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Open Access Article
1 - Determining the antibiotic resistance patterns of isolated Salmonella from broiler flocks to 28 antimicrobial agents used in Iran
Azizpour, A.* .The purpose of this study was to determine the serogroups of isolated Salmonella spp from broiler flocks and their drug resistance patterns to 28 antimicrobial agents commonly used in medicine and veterinary of Iran. One-hundred and eighty-nine samples were collected fr MoreThe purpose of this study was to determine the serogroups of isolated Salmonella spp from broiler flocks and their drug resistance patterns to 28 antimicrobial agents commonly used in medicine and veterinary of Iran. One-hundred and eighty-nine samples were collected from liver, heart and intestine of slaughtered broiler flocks in Ardabil province for identification of salmonella. The antibiotic resistance rate of isolates was determined using Kirby-Bauer method. Out of 189 samples examined, Salmonella was isolated from 22 samples (11.6%). Out of 22 salmonella isolates, fourteen (63.7%), seven (31.8%) and one (4.5%) isolates belonged to serogroups D, C and B, respectively. Multiple resistance was observed among isolates. The highest resistance was to tetracycline (100%), chlortetracycline (91%), streptomycin (91%), doxycycline (86.5%), nalidixic acid (81.8%), neomycin (77.3%), kanamycin (68.3%), furazolidone (63.7%), lincospectin (59.1%), flumequine (54.6%), penicillin (45.5%) and sulfamethoxazole+ trimethoprim (40.9%). Ten resistance patterns to ten antimicrobial agents commonly used in Iranian poultry industry were found in 22 Salmonella isolates 81.82% of isolates were resistant to more than two antibacterials. The results of this study showed that the frequency of resistance to the antimicrobial agents among avian salmonella isolates is a major public health concern. Manuscript profile -
Open Access Article
2 - Isolation and identification of gram-negative bacteria from patients with urinary tract infections referred to Savadkooh laboratory and determination of their drug resistance pattern from the beginning of October 1400 to the end of December 1400
seyedmohammadmahdi mousavi mojtaba fallah neda Jafari JushqhanToday, microorganisms have become resistant to drugs by causing genetic mutations. The aim of this study was to determine the antibiotic resistance of gram-negative bacteria isolated from urine culture in the laboratory of Savadkuh city. The cross-sectional descriptive MoreToday, microorganisms have become resistant to drugs by causing genetic mutations. The aim of this study was to determine the antibiotic resistance of gram-negative bacteria isolated from urine culture in the laboratory of Savadkuh city. The cross-sectional descriptive study was performed on gram-negative bacteria isolated from urinary tract infections in Savadkuh laboratory. Bacterial isolates were identified by microscopic examination, morphology and biochemical tests. Fifty samples of gram-negative bacteria causing urinary tract infections were isolated from 5 cases (10%) of men and 45 cases (90%) of women. According to the study, the two most common gram-negative bacteria in urinary tract infections are based on the percentage of infectivity in this study, which include: E.Coli (62%), and Klebsiella (30%) and other gram-negative (8%), They were isolated from urine samples. According to the antibiogram test, the most cases of resistance were related to the antibiotics ampicillin, nalidixic acid and cefazolin, respectively. On the other hand, the most sensitive drugs are nitrofurantoin, sulfamethoxazole trimethoprim and ciprofloxacin, respectively. Manuscript profile -
Open Access Article
3 - Prevalence and antibiotic profile of Escherichia coli in traditionally made ice cream in retails of Khoy
حامد Molaabaszadeh مسعود Molaazadeh نادر Hajizadeh نادر Mohammadzadeh Gheshlaghi Escherichia coli is among the most important intestinal foodborne bacterial pathogens. The objective of this study was to estimate the prevalence of E. coli in traditionally-made ice creams at retails of Khoy city. Moreover, the antibiotic profile of the is More Escherichia coli is among the most important intestinal foodborne bacterial pathogens. The objective of this study was to estimate the prevalence of E. coli in traditionally-made ice creams at retails of Khoy city. Moreover, the antibiotic profile of the isolates was investigated. For this, during April to September 2012, 150 ice cream samples collected from markets and confectionery shops. The samples were analyzed for the presence of E. coli. Afterwards, the antibiotic susceptibility and resistance of the isolates was evaluated on 10 different antibiotic using Kirby-Bauer test.According tothe results, 31.33% (47.150) of the samples were found positive for E. coli. The results of antibiogram test indicated that the highest level of sensitivity was determined for ceftizoxim (80.85%), ciprofloxacin (78.73%), and ceftriaxone (74.47%), respectively. In contrast, the most resistance antibiotics were amoxicillin (95.74%), oxacilin (82.98%), kanamycin (61.7%), respectively. The results revealed that the prevalence of E. coli, as the indication of fecal contamination, in traditionally made ice cream in Khoy retails and the antibiotic profile of the isolates were noticeable. Manuscript profile -
Open Access Article
4 - بررسی میزان الودگی و تعیین الگوی حساسیت و مقاومت انتی بیوتیکی کلی فرم های جداشده از فراورده های لبنی و گوشتی
عصمت خوری علی محمدی ثانی محمد رضا خضری -
Open Access Article
5 - بررسی شیوع و الگوی مقاومت آنتی بیوتیکی گونههای کمپیلوباکتر جدا شده از شیر خام گاو در ارومیه، ایران
مریم مصطفوی مسلم نیریز نقدهیگونههای کمپیلوباکتر شایعترین عامل اسهال باکتریایی در انسان میباشند. در تحقیق حاضر شیوع و مقاومت آنتیبیوتیکی گونههای کمپیلوباکتر جدا شده از شیر خام شهرستان ارومیه بررسیشدند. 80 نمونه شیر خام گاو بهصورت تصادفی و با شرایط سترون از خرده فروشیهای لبنیات سنتی مناطق م Moreگونههای کمپیلوباکتر شایعترین عامل اسهال باکتریایی در انسان میباشند. در تحقیق حاضر شیوع و مقاومت آنتیبیوتیکی گونههای کمپیلوباکتر جدا شده از شیر خام شهرستان ارومیه بررسیشدند. 80 نمونه شیر خام گاو بهصورت تصادفی و با شرایط سترون از خرده فروشیهای لبنیات سنتی مناطق مختلف ارومیه در سال 1397 جمعآوری شدند. نمونهها، ابتدا در آبگوشت پرستون تکمیلشده، غنیسازی و سپس در آگار تکمیل شده کمپیلوباکتر کشت و در دمای 42 درجهسلسیوس بهمدت 48 ساعت در شرایط میکروآیروفیل گرمخانهگذاری شدند. آزمایشهای بیوشیمیایی ازجمله هیدرولیز هیپورات و حساسیت یا مقاومت به نالیدیکسیک اسید برای شناسایی گونهی جدایهها انجام شدند. آزمایش حساسیت آنتیبیوتیکی روی جدایهها بهروش انتشار دیسک کربی-بائر انجام شد. 13 نمونه (25/16 درصد) آلوده به گونههای کمپیلوباکتر بودند. شیوع جدایههای کمپیلوباکتر ججونای (75/13 درصد) بالاتر از کمپیلوباکتر کولای (5/2 درصد) بود. جدایههای کمپیلوباکتر مقاومت بالا در برابر تتراسایکلین (100 درصد)، کوتریموکسازول (84 درصد)، آمپیسیلین، سفتریاکسون و کلرامفنیکل (2/69 درصد) نشان دادند درحالیکه آنها مقاومت متوسط به سیپروفلوکساسین و نیتروفورانتوئین (2/46 درصد) و مقاومت پایین به جنتامایسین (8/30 درصد) نشان دادند. همچنین 9 جدایه (2/69 درصد) مقاومت چند دارویی (MDR) نشان دادند. میتوان نتیجهگیری نمود که شیوع گونههای کمپیلوباکتر و سویههای MDR آنها در شیر خام گاو توزیعی ارومیه بالا میباشد. ارتقا سلامت دام و بهداشت شیر، جلوگیری از مصرف بیش از حد آنتیبیوتیکها در مزارع گاوان شیری و پاستوریزاسیون شیر پیشنهاد میگردد.کلمات کلیدی: شیرخام، گونههای کمپیلوباکتر، الگوی مقاومت آنتی بیوتیکی، ارومیه، ایران Manuscript profile