Due to the high consumption of amylase, the use of amylase-producing strains and optimizing the production conditions of this enzyme is an efficient way to produce this enzyme. Due to the fact that the amylase gene of Bacillus alkalitelluris has not been identified so f More
Due to the high consumption of amylase, the use of amylase-producing strains and optimizing the production conditions of this enzyme is an efficient way to produce this enzyme. Due to the fact that the amylase gene of Bacillus alkalitelluris has not been identified so far, it is important to achieve stable enzyme production conditions by the bacteria that produce these enzymes. One of the most important methods is to immobilization of the bacteria in suitable support so that the ability of these bacteria to produce enzymes is maintained. In this study, Bacillus alkalitelluris was first cultured in LB broth culture medium containing starch. The bacteria were then immobolized on agar and the amount of enzyme produced was measured using the DNS method. Finally, the amount of enzyme production when re-use stabilized cells in successive cultures was evaluated. According to the results, the bacterium Bacillus alkalitelluris immobilized in agar support is still able to produce alpha-amylase enzyme. Maximum alpha-amylase production was obtained after 24 hours. In addition, the immobilized bacteria remain after 4 times of changing the culture medium and are able to produce amylase enzyme so that up to 72 hours no significant change in enzyme production is observed. Due to the fact that the immobilized of Bacillus alkalitelluris in agar leads to the maintenance of alpha-amylase production capacity, so this method can be used in mass production of amylase enzyme.
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مقدمه و هدف: سیگناد گوگال یکی از داروهای گیاهی است که در طب آیورودا هند کاربرد فراوانی دارد. در تحقیق حاضر اثر عصاره آبی سیگناد گوگال بر بازدارندگی آلفا آمیلاز و خاصیت آنتی اکسیدانی آن مورد بررسی قرار گرفت. روش تحقیق: به منظور بررسی اثر آنتی اکسیدانی ، از More
مقدمه و هدف: سیگناد گوگال یکی از داروهای گیاهی است که در طب آیورودا هند کاربرد فراوانی دارد. در تحقیق حاضر اثر عصاره آبی سیگناد گوگال بر بازدارندگی آلفا آمیلاز و خاصیت آنتی اکسیدانی آن مورد بررسی قرار گرفت. روش تحقیق: به منظور بررسی اثر آنتی اکسیدانی ، از روش قدرت احیا کنندگی FRAP و برای تعیین فعالیت بازدارندگی آلفا آمیلاز از روش دنیترو-سالیسیلیک اسید استفاده شد. نتایج و بحث: نتایج نشان داد که سیگناد گوگال دارای پتانسیل بازدارندگی آلفا-آمیلاز و آنتی اکسیدان به روش FRAP با IC50 معادل 84 میکروگرم در میلی لیتر می باشد. توصیه کاربردی/ صنعتی: نتایج تحقیق حاضر بیانگر آن است که سیگناد گوگال ترکیبی از چند گیاه دارویی Commiphora mukul Emblica officinalis, Terminalia belerica, Terminalia chebula, Ricinus communis قابلیت بسیار مناسبی آنتی اکسیدانی و بازدارندگی آلفا-آمیلاز را دارد.
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The microfluidic aqueous two-phase system is a new method and is a suitable platform for the separation and recovery of biomaterials in the field of biotechnology. The combination of aqueous two-phase systems with microfluidic systems provides features that are not poss More
The microfluidic aqueous two-phase system is a new method and is a suitable platform for the separation and recovery of biomaterials in the field of biotechnology. The combination of aqueous two-phase systems with microfluidic systems provides features that are not possible or difficult to achieve in macro methods. According to studies, aqueous two-phase system can be considered as a low-cost, environmentally friendly, and effective method for the separation of biomolecules, but its combination with microfluidic systems adds to its attractiveness. In the studied system, due to its dimensions, it showed the potential ability to accelerate the design and separation of biological processes. Alpha-amylase recovery was investigated with an aqueous two-phase polyethylene glycol/sodium citrate system on a glass microfluidic device made by the Co2-laser method. Effective parameters were tested with different values of concentration (125-150 mg / L) and flow rate (0.8-2 mL / h). The response surface method (RSM) was used to optimally determine the operational parameters. Transfer of alpha-amylase from the salt phase to the polyethylene glycol phase was performed with a parallel flow pattern. The values obtained at the optimal point also had a small error compared to the predicted value of the experimental design equations. The use of the microfluidic system studied in this study due to the micro dimensions will increase the recovery rate compared to macro systems, as well as the reduction of the time of this process in the micro dimensions compared to macro dimensions was significant.
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